A0LSS7 (SYE_ACIC1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	Acel_0714

Organism

Acidothermus cellulolyticus (strain ATCC 43068 / 11B) [Complete proteome] [HAMAP]

Taxonomic identifier

351607 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Frankineae › Acidothermaceae › Acidothermus

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 504	504	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_1000057189
Regions
	Motif	9 – 19	11	"HIGH" region HAMAP MF_00022_B
	Motif	248 – 252	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	251	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0LSS7 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 89695C333DE6F8C8	FASTA	504	56,459
	10 20 30 40 50 60 MTVRVRVAPS PTGDPHVGTA YMSLFNLAFA RRHGGAFVLR IEDTDRSRYV ADSEQQIFDS 70 80 90 100 110 120 LRWLGLDWDE GPDKGGPYGP YRQSERLDTY RPYVDQLLAS GHAYYCWCSP ERLAAMREEQ 130 140 150 160 170 180 QRRRQPPGYD RLCYGKTREE RARLGGFSER PVVRMLIPDD VPLTFPDLIR GQVSAPRPDD 190 200 210 220 230 240 QVILKADGFP TYHFAVVVDD HLMAITHVVR GEEWISSTPK HLLLYDWLGW PRPQFAHMPL 250 260 270 280 290 300 LRNPDKSKIS KRKNPAARLL WFKEQGYLPE ALRNFLALMG YSMPDGREVF SFDEMVAEFD 310 320 330 340 350 360 WSRVNPVGPV FDVTKLDWLN GHYIRSLPVD DLAERLIPYL QEARLIGTPV TPREDALLRA 370 380 390 400 410 420 AAPLVQERIA HLTEAAELLA FLLRPEEEFG IEPDAAQRVL GRDAAASLRA AIDALEPLAD 430 440 450 460 470 480 WTASAIHAAL QAALVDGLGL KPRHAFTPVR VAITGRTVSP PLFESMELLG RDRSLARLRR 490 500 AAAIADAGGA DSAGVGPGSA VRTD

References

[1]

"Complete sequence of Acidothermus cellulolyticus 11B." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Zharchuk I., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Berry A.M., Adney W.S., Normand P., Leu D., Pujic P., Richardson P. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43068 / 11B.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000481 Genomic DNA. Translation: ABK52487.1.
RefSeq	YP_872473.1. NC_008578.1.
3D structure databases
ProteinModelPortal	A0LSS7.
ModBase	Search...
Protein-protein interaction databases
STRING	A0LSS7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4485134.
GenomeReviews	Gene locus Acel_0714 in contig CP000481_GR.
KEGG	ace:Acel_0714.
PATRIC	20671822. VBIAciCel132453_0756.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	VRFRMPD.
PhylomeDB	A0LSS7.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	ACEL351607:ACEL_0714-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_ACIC1

Accession

Primary (citable) accession number: A0LSS7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	December 12, 2006
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program