A0LSN9 (A0LSN9_ACIC1) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB HAMAP MF_00685
EC=2.4.1.18 HAMAP MF_00685

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase HAMAP MF_00685
Alpha-(1->4)-glucan branching enzyme HAMAP MF_00685
Glycogen branching enzyme HAMAP MF_00685

Gene names

Name:	glgB HAMAP MF_00685
Ordered Locus Names:	Acel_0676

Organism

Acidothermus cellulolyticus (strain ATCC 43068 / 11B) [Complete proteome] [HAMAP]

Taxonomic identifier

351607 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Frankineae › Acidothermaceae › Acidothermus

Protein attributes

Sequence length	727 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685 SAAS SAAS006407
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685 SAAS SAAS006407
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily. HAMAP MF_00685

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis HAMAP MF_00685 SAAS SAAS006407 Glycogen metabolism HAMAP MF_00685
Molecular function	Glycosyltransferase HAMAP MF_00685 SAAS SAAS006407 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	408	1	Nucleophile By similarity HAMAP MF_00685
	Active site	461	1	Proton donor By similarity HAMAP MF_00685

Sequences

	Sequence		Length	Mass (Da)
	A0LSN9 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 76AA04BA37CA2877	FASTA	727	82,438
	10 20 30 40 50 60 MSRSEPRPAP VDHGELDRLV SGAHHNPHGI LGAHHHDGVV TFRTFRPFAT RVTVLVAGER 70 80 90 100 110 120 IPLVHEYRGI WAGAWESSAL PDYRLEVVYD DGFPHVVDEP YRYLPTLGEL DLHLISEGRH 130 140 150 160 170 180 EELWRVLGAH ERRYDTPNGP VTGTSFAVWA PNARGIRIAG DFNHWNPTAH PMRSLGSSGI 190 200 210 220 230 240 WELFIPGVGS GARYKYHVLG ADGRWREKAD PMAFATEVPP AQASVVFTSH YEWGDAEWLA 250 260 270 280 290 300 RRAQTDWLHA PMSIYEVHLG SWRAGLGYRE LADELVRYVT DLGFTHVEFL PVAEHPFSGS 310 320 330 340 350 360 WGYQVTAYFA PTARYGSPDD FRYLIDRLHQ ANIGVIVDWV PAHFPKDDWA LARFDGTALY 370 380 390 400 410 420 EHEDPQLGEH PDWGTLIFNF GRREVRNFLV ANALFWLEEM HVDGLRVDAV ASMLYLDYSR 430 440 450 460 470 480 PAGAWRPNIY GGRENLEAIS FLQEMNATAY KRVPGIVTVA EESTAWPGVT RPTYVGGLGF 490 500 510 520 530 540 GFKWNMGWMH DTLQYMSRDP IYRSYHHNEM TFAMMYAYSE NFILPLSHDE VVHGKGSLLH 550 560 570 580 590 600 KMPGDRWQQM ANLRAYLAFM WAHPGKQLLF MGGEFAQDNE WSEERSLDWW LLQYREHSSV 610 620 630 640 650 660 QQLVRDLNAL YRAHPALWRT DDDPSAFRWI DANDNQNNTF SFARTAPDGS TIVCVANFAG 670 680 690 700 710 720 VPHEEYHLGF PSPGRWREIL NTDAEIYNGS GVGNLGGIDA VATPSHGLPA SAVIRVPPLA TIWFAQP

References

[1]

"Complete sequence of Acidothermus cellulolyticus 11B." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Zharchuk I., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Berry A.M., Adney W.S., Normand P., Leu D., Pujic P., Richardson P. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43068 / 11B.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000481 Genomic DNA. Translation: ABK52449.1.
RefSeq	YP_872435.1. NC_008578.1.
3D structure databases
ProteinModelPortal	A0LSN9.
SMR	A0LSN9. Positions 120-726.
ModBase	Search...
Protein-protein interaction databases
STRING	A0LSN9.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4486232.
GenomeReviews	Gene locus Acel_0676 in contig CP000481_GR.
KEGG	ace:Acel_0676.
PATRIC	20671746. VBIAciCel132453_0718.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0296.
HOGENOM	HBG287139.
OMA	DGTCLYE.
PhylomeDB	A0LSN9.
ProtClustDB	PRK05402.
Family and domain databases
HAMAP	MF_00685. GlgB. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz. IPR006048. A-amylase_b_C. IPR015902. Alpha_amylase. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 2 hits.
KO	K00700.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF13. PTHR10357:SF13. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
TIGRFAMs	TIGR01515. Branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A0LSN9_ACIC1

Accession

Primary (citable) accession number: A0LSN9

Entry history

Integrated into UniProtKB/TrEMBL:	December 12, 2006
Last sequence update:	December 12, 2006
Last modified:	December 14, 2011
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)