A0LQZ8 (SYC_ACIC1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine--tRNA ligase
EC=6.1.1.16

Alternative name(s):
Cysteinyl-tRNA synthetase
Short name=CysRS

Gene names

Name:	cysS
Ordered Locus Names:	Acel_0082

Organism

Acidothermus cellulolyticus (strain ATCC 43068 / 11B) [Complete proteome] [HAMAP]

Taxonomic identifier

351607 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Frankineae › Acidothermaceae › Acidothermus

Protein attributes

Sequence length	469 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00041
Subunit structure	Monomer By similarity. HAMAP MF_00041
Subcellular location	Cytoplasm HAMAP MF_00041.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cysteine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 469	469	Cysteine--tRNA ligase HAMAP MF_00041		PRO_1000006559
Regions
	Motif	31 – 41	11	"HIGH" region HAMAP MF_00041
	Motif	263 – 267	5	"KMSKS" region HAMAP MF_00041
Sites
	Metal binding	29	1	Zinc By similarity
	Metal binding	207	1	Zinc By similarity
	Metal binding	232	1	Zinc By similarity
	Metal binding	236	1	Zinc By similarity
	Binding site	266	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0LQZ8 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: CB47412C9A2DAA30	FASTA	469	51,954
	10 20 30 40 50 60 MPLRLHDTRS RGLREFQPAD PPRVTMYVCG ATVQAPPHIG HLRSGLVFDV LVRWLAVHGY 70 80 90 100 110 120 DVVFCRNVTD IDDKILRVAA AEGIPWWQVA YRNYRLFDSA YRLLGCVPPT VEPWATGHIP 130 140 150 160 170 180 QIIALIRRLI DAGHAYPAGG DVYFAVRSFP AYGGLSGRDP DALQAGESDG VHKRDPLDFA 190 200 210 220 230 240 LWKGAKPGEP SWESPWGPGR PGWHIECSAM STTYLGSTFD IHGGGLDLLF PHHENECAQS 250 260 270 280 290 300 QAAGDGFARY WLHNGLVTLR GEKMSKSLGN VLSMDQLLEL VRPIELRYYL IGAHYRSTLD 310 320 330 340 350 360 FSEESLEEAA AAYRRIEGFL LRAQELVGEL PAADPQTGLI DRLPPEFVAA LDDDLAVPRA 370 380 390 400 410 420 LAVMHSVVRQ GNAALAETDK TAVRRFLGEV TAMLDVMGLR PPDDGSRLDA LRRVVDNLAR 430 440 450 460 LVLDEREAAR QRRDFATADA LRARLTDAGI VVEDTPSGPR WHLRDLDLP

References

[1]

"Complete sequence of Acidothermus cellulolyticus 11B." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Zharchuk I., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Berry A.M., Adney W.S., Normand P., Leu D., Pujic P., Richardson P. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43068 / 11B.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000481 Genomic DNA. Translation: ABK51858.1.
RefSeq	YP_871844.1. NC_008578.1.
3D structure databases
ProteinModelPortal	A0LQZ8.
ModBase	Search...
Protein-protein interaction databases
STRING	A0LQZ8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4484674.
GenomeReviews	Gene locus Acel_0082 in contig CP000481_GR.
KEGG	ace:Acel_0082.
PATRIC	20670466. VBIAciCel132453_0087.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0215.
HOGENOM	HBG327651.
OMA	DSHIGHA.
Enzyme and pathway databases
BioCyc	ACEL351607:ACEL_0082-MONOMER.
Family and domain databases
HAMAP	MF_00041. Cys_tRNA_synth. [Tree]
InterPro	IPR015803. Cys-tRNA-synt. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR024909. Cys-tRNA/MSH_ligase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01883.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
SMART	SM00840. DALR_2. 1 hit. [Graphical view]
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00435. CysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_ACIC1

Accession

Primary (citable) accession number: A0LQZ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	December 12, 2006
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program