A0LPG9 (ISPE_SYNFM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	Sfum_3651

Organism

Syntrophobacter fumaroxidans (strain DSM 10017 / MPOB) [Complete proteome] [HAMAP]

Taxonomic identifier

335543 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Syntrophobacterales › Syntrophobacteraceae › Syntrophobacter

Protein attributes

Sequence length	297 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 297	297	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000335765
Regions
	Nucleotide binding	103 – 113	11	ATP Potential
Sites
	Active site	19	1	By similarity
	Active site	145	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0LPG9 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 962A56946CB8B314	FASTA	297	32,107
	10 20 30 40 50 60 MRGIRKGHAL ALRVGTPAKI NLWLEIVRKR EDGYHDLSSL MLPVAVYDHV EVAWSHEGGV 70 80 90 100 110 120 RLSCDSAEVP DDRGNLAWRA ADAYLEAAGV TRGVSIRLEK NIPVGAGMGG GSSDAAAVLL 130 140 150 160 170 180 ALNRLSENPL SEAVLHRLAV GLGADVPFFL HCVPALATGI GERLLPVHGI PDYPLLLVKP 190 200 210 220 230 240 PISVSTGWVY GSLKLTRGES RIKLRSFLDR PWHLSEVLRN DLETVTLNEY PQLGQIKCWL 250 260 270 280 290 MENGAVGSLM SGSGPTVFGV FAEQRLADGA GELARRVWKD CRVDVTRVLG TPVSARS

References

[1]

"Complete sequence of Syntrophobacter fumaroxidans MPOB." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Goltsman E.G., Martinez M., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Boone D.R. , Brockman F., Culley D., Ferry J., Gunsalus R., McInerney M.J., Morrison M., Plugge C., Rohlin L., Scholten J., Sieber J., Stams A.J.M., Worm P., Henstra A.M., Richardson P. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 10017 / MPOB.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000478 Genomic DNA. Translation: ABK19321.1.
RefSeq	YP_847756.1. NC_008554.1.
3D structure databases
ProteinModelPortal	A0LPG9.
ModBase	Search...
Protein-protein interaction databases
STRING	A0LPG9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4458041.
GenomeReviews	Gene locus Sfum_3651 in contig CP000478_GR.
KEGG	sfu:Sfum_3651.
NMPDR	fig|335543.6.peg.3768.
PATRIC	23853583. VBISynFum78438_4343.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1947.
HOGENOM	HBG734593.
OMA	VSTAEVY.
PhylomeDB	A0LPG9.
Enzyme and pathway databases
BioCyc	SFUM335543:SFUM_3651-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_SYNFM

Accession

Primary (citable) accession number: A0LPG9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	December 12, 2006
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program