Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutamyl-tRNA(Gln) amidotransferase subunit A

Gene

gatA

Organism
Syntrophobacter fumaroxidans (strain DSM 10017 / MPOB)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).UniRule annotation

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei78Charge relay systemUniRule annotation1
Active sitei153Charge relay systemUniRule annotation1
Active sitei177Acyl-ester intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit AUniRule annotation (EC:6.3.5.7UniRule annotation)
Short name:
Glu-ADT subunit AUniRule annotation
Gene namesi
Name:gatAUniRule annotation
Ordered Locus Names:Sfum_3503
OrganismiSyntrophobacter fumaroxidans (strain DSM 10017 / MPOB)
Taxonomic identifieri335543 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaSyntrophobacteralesSyntrophobacteraceaeSyntrophobacter
Proteomesi
  • UP000001784 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000159201 – 486Glutamyl-tRNA(Gln) amidotransferase subunit AAdd BLAST486

Interactioni

Subunit structurei

Heterotrimer of A, B and C subunits.UniRule annotation

Protein-protein interaction databases

STRINGi335543.Sfum_3503.

Structurei

3D structure databases

ProteinModelPortaliA0LP22.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the amidase family. GatA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C3P. Bacteria.
COG0154. LUCA.
HOGENOMiHOG000116699.
KOiK02433.
OMAiKEYFGAG.
OrthoDBiPOG091H00O9.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA. 1 hit.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0LP22-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPYALTMHE LHDLLVRKEL SVTETLTSFL TRIETLDPRL NSYLSVLAES
60 70 80 90 100
SLAEAGRFDR GERDLHASPL AGIPLAIKDV LCMQGTVTTC GSRILENFVP
110 120 130 140 150
PYDGTVIARL REAGAIFLGK TNMDEFAMGS STENSAYGVT RNPWDRERVP
160 170 180 190 200
GGSSGGSAAA VAADLCSGSL GTDTGGSIRQ PASFCGVVGL KPTYGRVSRF
210 220 230 240 250
GLVAFASSLD QIGPITKDVE DAAILLQAIA GHDRRDSTSV DHPVPDYRAS
260 270 280 290 300
LREPIKGLRL GIPKEYFVHG MHPEIADSVQ RAINVCLQLG AEVGEVSLPH
310 320 330 340 350
TGYGVAAYYI IAPAEASSNL ARYDGVKYGL RVPDARDLIG MYRTSRSQGF
360 370 380 390 400
GAEVKRRIML GTYVLSAGYY DAYYTKASQA RTLIRKDFLD AFDSFDALLA
410 420 430 440 450
PVAPVPAFKI GEKSDDPLQM YLNDALTLPA SLAGVPGISV PCGFSGEGLP
460 470 480
IGLQILGPHF REDLLLRIAY QFEQATAHHL ARPDSI
Length:486
Mass (Da):52,331
Last modified:December 12, 2006 - v1
Checksum:i32D6018FA9A9A6CF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000478 Genomic DNA. Translation: ABK19174.1.
RefSeqiWP_011700299.1. NC_008554.1.

Genome annotation databases

EnsemblBacteriaiABK19174; ABK19174; Sfum_3503.
KEGGisfu:Sfum_3503.
PATRICi23853203. VBISynFum78438_4154.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000478 Genomic DNA. Translation: ABK19174.1.
RefSeqiWP_011700299.1. NC_008554.1.

3D structure databases

ProteinModelPortaliA0LP22.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi335543.Sfum_3503.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK19174; ABK19174; Sfum_3503.
KEGGisfu:Sfum_3503.
PATRICi23853203. VBISynFum78438_4154.

Phylogenomic databases

eggNOGiENOG4105C3P. Bacteria.
COG0154. LUCA.
HOGENOMiHOG000116699.
KOiK02433.
OMAiKEYFGAG.
OrthoDBiPOG091H00O9.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA. 1 hit.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGATA_SYNFM
AccessioniPrimary (citable) accession number: A0LP22
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: December 12, 2006
Last modified: November 2, 2016
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.