ID   FTHS_SYNFM              Reviewed;         587 AA.
AC   A0LLR3;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   12-DEC-2006, sequence version 1.
DT   27-MAR-2024, entry version 87.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543};
GN   OrderedLocusNames=Sfum_2687;
OS   Syntrophobacter fumaroxidans (strain DSM 10017 / MPOB).
OC   Bacteria; Thermodesulfobacteriota; Syntrophobacteria; Syntrophobacterales;
OC   Syntrophobacteraceae; Syntrophobacter.
OX   NCBI_TaxID=335543;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 10017 / MPOB;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Pitluck S., Goltsman E.G.,
RA   Martinez M., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA   Kim E., Boone D.R., Brockman F., Culley D., Ferry J., Gunsalus R.,
RA   McInerney M.J., Morrison M., Plugge C., Rohlin L., Scholten J., Sieber J.,
RA   Stams A.J.M., Worm P., Henstra A.M., Richardson P.;
RT   "Complete sequence of Syntrophobacter fumaroxidans MPOB.";
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6R)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:195366, ChEBI:CHEBI:456216;
CC         EC=6.3.4.3; Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR   EMBL; CP000478; ABK18365.1; -; Genomic_DNA.
DR   RefSeq; WP_011699532.1; NC_008554.1.
DR   AlphaFoldDB; A0LLR3; -.
DR   SMR; A0LLR3; -.
DR   STRING; 335543.Sfum_2687; -.
DR   KEGG; sfu:Sfum_2687; -.
DR   eggNOG; COG2759; Bacteria.
DR   HOGENOM; CLU_003601_3_3_7; -.
DR   InParanoid; A0LLR3; -.
DR   OrthoDB; 9761733at2; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000001784; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.30.1510.10; Domain 2, N(10)-formyltetrahydrofolate synthetase; 1.
DR   Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW   Reference proteome.
FT   CHAIN           1..587
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_0000293071"
FT   BINDING         73..80
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   587 AA;  64624 MW;  174E693ECDA3007E CRC64;
     MPYDATKMMD WQISEEAEKD MPSPWQWQEK LGLLKEEILP MGRLCKLDFL KIMNRLKDKP
     DGKYIEVTAI TPTPLGEGKS TTSVGLMEGL GKRGVNVGGC LRQPSGGPTM NIKGTAAGGG
     NALLIPMTEF SMGLTGDIND IMNAHNLAMV ALTARMQHER NYTDEQLDRL TKMRRLHIDP
     TRVEMGWIMD FCAQALRNII IGIGGRQDGY MMQSKFGIAV SSELMAILSI VKDLPDLRKR
     LNEITVAFDR RGNPVTTGDL EVGGAMTAFM RNTINPTLMC TAEYQPCMVH AGPFANIAVG
     QSSIIADRIG LKMFDYHVTE SGFAADIGFE KFWNVKCRYS GLKPHVSVLT TTIRALKMHG
     GGPKVVAGLA MPEEYTKENL KLLEKGIVNM VHHINTIRKS GMNPVVCINA FHTDTKDEIA
     LVRKHAEAAG ARCALSEHWA KGGEGALEFA DAVIDACKQE SQFKFLYPLE MKLRDRVSTV
     AREVYGADGV SWTPDAEAKA KMLENDPKYD DYATMMVKTH LSLTHDPSVK GVPKGWVLPI
     RDVLIYSGAK FLCPCAGTIS LMPGTSSNPA FRRIDVDVNT GKVKGLF
//