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A0LK15 (SYL_SYNFM) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Sfum_2084
OrganismSyntrophobacter fumaroxidans (strain DSM 10017 / MPOB) [Complete proteome] [HAMAP]
Taxonomic identifier335543 [NCBI]
Taxonomic lineageBacteriaProteobacteriaDeltaproteobacteriaSyntrophobacteralesSyntrophobacteraceaeSyntrophobacter

Protein attributes

Sequence length829 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 829829Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009454

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif584 – 5885"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5871ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A0LK15 [UniParc].

Last modified December 12, 2006. Version 1.
Checksum: 5AA680E8AE916D5A

FASTA82993,354
        10         20         30         40         50         60 
MDYKYAPKRI EKKWQEHWER EKLFEVSEIP GREKFYLLEM FPYPSGRIHM GHVRNYSIGD 

        70         80         90        100        110        120 
VVARFLRMRG YNVLHPMGWD AFGMPAENAA IKAKTHPARW TYENIAYMRS QLKQLGFSYD 

       130        140        150        160        170        180 
WSREFATCDV SYYRWEQLFF LKMYEKGLAY KRSAYVNWCG TCLTVLANEQ VEGGACWRCD 

       190        200        210        220        230        240 
QPVVQKEMEQ WFFKITDYVE ELLDYTHRLP GWPERVLTMQ QNWIGKSLGS KLLFPLASGD 

       250        260        270        280        290        300 
GSITVFTTRA DTLFGATFMS LAPEHPLVEG LCRGNPQESE VLRFVQAAKQ AKRNDREAEL 

       310        320        330        340        350        360 
LEKEGVFTGS CCINPVTGAK MPIYVANFVV MEYGTGAVMA VPAHDQRDFE FARKYGLPVK 

       370        380        390        400        410        420 
VVIKPADAAA APAAQELSAA FEDDGVLVDS GAYSGMASAE ARTAITADLA GKGLGEQTVQ 

       430        440        450        460        470        480 
YRLRDWGISR QRYWGAPIPI VYCDKCGTVP VPEKDLPVVL PTDVALLPNG ASPLPAHAPF 

       490        500        510        520        530        540 
LNTDCPRCGG PARRETDTMD TFVESSWYFA RFACARYDQG PLDLPKVKYW MPVDQYIGGI 

       550        560        570        580        590        600 
EHAVLHLLYS RFFVKVLRDM GALEVDEPFR NLLTQGMVIK DGAKMSKSKG NVVDPDDMIK 

       610        620        630        640        650        660 
AYGADTVRLF CLFASPPEKD LEWSDQGVEG SFRFLSRIWR LVSDNLDALR SAPRHNGGGA 

       670        680        690        700        710        720 
LPEPLEALHR KTHQTIKKVT EDIRDRFHFN TAIAAIMELV NQIYQVVEGG SRAGNIWPVV 

       730        740        750        760        770        780 
KEAVEALILL VSPMAPHIAE EIWHELGHTR SVLLEPWPEW SGKALQAEEV MLVVQVNGRL 

       790        800        810        820 
RSRITVPSDA TPEQMEAAAL ADSRVQEFIA GKPVRKVVVV PKKIINVVV 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000478 Genomic DNA. Translation: ABK17767.1.
RefSeqYP_846202.1. NC_008554.1.

3D structure databases

ProteinModelPortalA0LK15.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING335543.Sfum_2084.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABK17767; ABK17767; Sfum_2084.
GeneID4459613.
KEGGsfu:Sfum_2084.
PATRIC23849777. VBISynFum78438_2458.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycSFUM335543:GH6P-2122-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_SYNFM
AccessionPrimary (citable) accession number: A0LK15
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: December 12, 2006
Last modified: April 16, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries