Reviewed, UniProtKB/Swiss-Prot A0LJM5 (NUBCD_SYNFM)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit B/C/D
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit B/C/D
    NDH-1 subunit B/C/D

Gene names

Name:	nuoBCD
Synonyms:	nuoB, nuoC, nuoD
Ordered Locus Names:	Sfum_1942

Organism

Syntrophobacter fumaroxidans (strain DSM 10017 / MPOB) [Complete proteome] [HAMAP]

Taxonomic identifier

335543 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Syntrophobacterales › Syntrophobacteraceae › Syntrophobacter

Protein attributes

Sequence length	787 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_01356
Subunit structure	NDH-1 is composed of about 13 different subunits. Subunits nuoBCD, E, F, and G constitute the peripheral sector of the complex By similarity.
Subcellular location	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side By similarity.
Sequence similarities	In the N-terminal section; belongs to the complex I 20 kDa subunit family. In the central section; belongs to the complex I 30 kDa subunit family. In the C-terminal section; belongs to the complex I 49 kDa subunit family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: HAMAP photosynthesis, light reaction Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	internal side of plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: InterPro NAD or NADH binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: HAMAP quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 787	787	NADH-quinone oxidoreductase subunit B/C/D HAMAP MF_01356		PRO_0000358703
Regions
	Region	1 – 149	149	NADH dehydrogenase I subunit B By similarity
	Region	225 – 380	156	NADH dehydrogenase I subunit C By similarity
	Region	407 – 787	381	NADH dehydrogenase I subunit D By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0LJM5-1 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 0891300B37D05455	FASTA	787	88,858
	10 20 30 40 50 60 MSDRFLSAAD FVLNWSRKYS LWPLFFGLSC CFVEEATAFT PRYDMARFGA EVLRPSPRQA 70 80 90 100 110 120 DLLIVSGTVF KKIAPVVLRL YEQMAEPKWV ISMGSCSNCG GMYDVYSVVQ GIDQILPVDV 130 140 150 160 170 180 YIPGCPPRPE AVLQGLTLLQ KKIAAEERPA RSVLHLPGGS QGSTRPVLVD GATKSRDPRG 190 200 210 220 230 240 PGMEGTVIRG TANTPPAFTG SRSDLMWTPP ARRIESSERE KELARVLSER FGDAVREEPF 250 260 270 280 290 300 TSDMPTFHVE TNRLKDVLGF LKKEASPRFL RLDDLTAVDE SARRDRSAYP DWTMVYHLLS 310 320 330 340 350 360 FEPAGRVRLK TGLHGRQPAL PSITDIWPSA NWYEREVYDL FGIRFEGHPN LRRIMMPHDW 370 380 390 400 410 420 EGHPLRKDYP GRATQMAPYT LGDARVHQPP DGGIFTKDPG GDRLVLNIGP SHVSTHGLLR 430 440 450 460 470 480 YVVSLDGEEI SDLELEIGYH HRGVEKIGER QTWHQFIPYC ARVDYLAGAA NDLPYVMAVE 490 500 510 520 530 540 TLADIKVPER AQVIRVLLSE LFRLSNHLVW FATYAHDVGA MTPNFYTFTE REMILDIVEL 550 560 570 580 590 600 ITGGRLHPSW FRLGGVAADL PEGWKEAVDA LVRIFPGRLR EYESLIRKNP IFKARTQGVG 610 620 630 640 650 660 RISREDAVDW GVSGPNLRAC GLEWDLRRKF PYSGYENFEF EVPTAVEGDC YARYLVRVEE 670 680 690 700 710 720 MRQSLRIIEQ AAANMPSGRH VTDDYRYVIP DRRDTLKNIE SLIHHFVNVT RGPRIPKGEA 730 740 750 760 770 780 YVSCEIPRGE QGYYVVGDGL GYAYRMRIRG PGFANVQVMP LLARGESIAD LIAIIGSVDY ILPDIDR

References

[1]

"Complete sequence of Syntrophobacter fumaroxidans MPOB." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Goltsman E.G., Martinez M., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Boone D.R. , Brockman F., Culley D., Ferry J., Gunsalus R., McInerney M.J., Morrison M., Plugge C., Rohlin L., Scholten J., Sieber J., Stams A.J.M., Worm P., Henstra A.M., Richardson P. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000478 Genomic DNA. Translation: ABK17627.1.
RefSeq	YP_846062.1.
3D structure databases
SMR	A0LJM5. Positions 285-400.
ModBase	Search...
Protein-protein interaction databases
STRING	A0LJM5.
Genome annotation databases
GeneID	4459747.
GenomeReviews	Gene locus Sfum_1942 in contig CP000478_GR.
KEGG	sfu:Sfum_1942.
NMPDR	fig|335543.6.peg.2000.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	PPTWEGH.
Family and domain databases
HAMAP	MF_01356. Fused. [Tree] MF_01359. Fused. [Tree]
InterPro	IPR010219. NADH_DH_1_dsu. IPR010218. NADH_DH_csu. IPR001135. NADH_Q_OxRdtase_suD. IPR006137. NADH_UbQ_OxRdtase-like_20kDa. IPR006138. NADH_UbQ_OxRdtase_20kDa_su. IPR001268. NADH_UbQ_OxRdtase_30kDa_su. IPR014029. NADH_UbQ_OxRdtase_49kDa_CS. IPR020396. NADH_UbQ_OxRdtase_CS. [Graphical view]
Pfam	PF00329. Complex1_30kDa. 1 hit. PF00346. Complex1_49kDa. 1 hit. PF01058. Oxidored_q6. 1 hit. [Graphical view]
ProDom	PD001581. Complex1_30K. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01957. nuoB_fam. 1 hit. TIGR01961. NuoC_fam. 1 hit. TIGR01962. NuoD. 1 hit.
PROSITE	PS01150. COMPLEX1_20K. False negative. PS00542. COMPLEX1_30K. 1 hit. PS00535. COMPLEX1_49K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUBCD_SYNFM

Accession

Primary (citable) accession number: A0LJM5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	December 12, 2006
Last modified:	November 3, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)