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A0LJL8 (NUON2_SYNFM) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADH-quinone oxidoreductase subunit N 2

EC=1.6.99.5
Alternative name(s):
NADH dehydrogenase I subunit N 2
NDH-1 subunit N 2
Gene names
Name:nuoN2
Ordered Locus Names:Sfum_1935
OrganismSyntrophobacter fumaroxidans (strain DSM 10017 / MPOB) [Complete proteome] [HAMAP]
Taxonomic identifier335543 [NCBI]
Taxonomic lineageBacteriaProteobacteriaDeltaproteobacteriaSyntrophobacteralesSyntrophobacteraceaeSyntrophobacter

Protein attributes

Sequence length472 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity. HAMAP-Rule MF_00445

Catalytic activity

NADH + quinone = NAD+ + quinol. HAMAP-Rule MF_00445

Subunit structure

NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity.

Subcellular location

Cell inner membrane; Multi-pass membrane protein By similarity HAMAP-Rule MF_00445.

Sequence similarities

Belongs to the complex I subunit 2 family.

Ontologies

Keywords
   Biological processTransport
   Cellular componentCell inner membrane
Cell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   LigandNAD
Ubiquinone
   Molecular functionOxidoreductase
   PTMQuinone
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processATP synthesis coupled electron transport

Inferred from electronic annotation. Source: InterPro

transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionNADH dehydrogenase (ubiquinone) activity

Inferred from electronic annotation. Source: InterPro

quinone binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 472472NADH-quinone oxidoreductase subunit N 2 HAMAP-Rule MF_00445
PRO_5000166272

Regions

Transmembrane3 – 2321Helical; Potential
Transmembrane34 – 5421Helical; Potential
Transmembrane67 – 8721Helical; Potential
Transmembrane106 – 12621Helical; Potential
Transmembrane156 – 17621Helical; Potential
Transmembrane198 – 21821Helical; Potential
Transmembrane233 – 25321Helical; Potential
Transmembrane263 – 28321Helical; Potential
Transmembrane291 – 31121Helical; Potential
Transmembrane317 – 33721Helical; Potential
Transmembrane360 – 38021Helical; Potential
Transmembrane398 – 41821Helical; Potential
Transmembrane441 – 46121Helical; Potential

Sequences

Sequence LengthMass (Da)Tools
A0LJL8 [UniParc].

Last modified December 12, 2006. Version 1.
Checksum: A9F7BBF6C1DCCD73

FASTA47250,331
        10         20         30         40         50         60 
MNWMSFAPEL ITLTSALWFL LLSMTARSDP KREHVAALVL SALGLAACLA SVGAEGYCFA 

        70         80         90        100        110        120 
GAYKVDLFSQ VFKVLLAAGL FLIVTLCGEL SDIEERNRRE FYVLLFVCTL AMMLLVGANH 

       130        140        150        160        170        180 
FLVVFISLEL SSYSLYVLVA LRRDRGLGLE AGIKYFLVGI FASGVMIFGL ALLYGASGIA 

       190        200        210        220        230        240 
ALDGMARVLP GIIHQPAVVI GLLLTLSGFF FKLAVFPFHF WAPDAYQGAA NQVSAYIATA 

       250        260        270        280        290        300 
SKVAAIGVLV RVIASAGDGG TYLVHVLAVL SVVSMTVGNL AAIAQQDLKR LLAYSTVAHA 

       310        320        330        340        350        360 
GYVLIGVLSM NPAGYSAAVF YAFALLVMKF TAFLVLVEVA SDGGNLRVEE LAGLHRRSPI 

       370        380        390        400        410        420 
LALALMVSLF SLAGIPPTVG FTGKFLVFVA AMGEGHFTLV LIAMINVVIS LYYYLLVIKA 

       430        440        450        460        470 
AYLLEPRQEL PALRVSPPLK LLSGVLVIAM VAAGFFPNQI IRVAESAAKA LL 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000478 Genomic DNA. Translation: ABK17620.1.
RefSeqYP_846055.1. NC_008554.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING335543.Sfum_1935.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABK17620; ABK17620; Sfum_1935.
GeneID4459740.
KEGGsfu:Sfum_1935.
PATRIC23849405. VBISynFum78438_2273.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1007.
HOGENOMHOG000100794.
KOK00343.
OMAAYLRTRN.
OrthoDBEOG64JFNZ.
ProtClustDBCLSK765178.

Enzyme and pathway databases

BioCycSFUM335543:GH6P-1972-MONOMER.

Family and domain databases

HAMAPMF_00445. NDH1_NuoN_1.
InterProIPR010096. NADH-Q_OxRdtase_suN/2.
IPR001750. NADH_UbQ/plastoQ_OxRdtase.
[Graphical view]
PfamPF00361. Oxidored_q1. 1 hit.
[Graphical view]
TIGRFAMsTIGR01770. NDH_I_N. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNUON2_SYNFM
AccessionPrimary (citable) accession number: A0LJL8
Entry history
Integrated into UniProtKB/Swiss-Prot: February 9, 2010
Last sequence update: December 12, 2006
Last modified: February 19, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families