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Protein

NADH-quinone oxidoreductase subunit N 2

Gene

nuoN2

Organism
Syntrophobacter fumaroxidans (strain DSM 10017 / MPOB)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.UniRule annotation

Catalytic activityi

NADH + quinone = NAD+ + quinol.UniRule annotation

GO - Molecular functioni

  1. NADH dehydrogenase (ubiquinone) activity Source: InterPro
  2. quinone binding Source: UniProtKB-KW

GO - Biological processi

  1. ATP synthesis coupled electron transport Source: InterPro
  2. transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Transport

Keywords - Ligandi

NAD, Ubiquinone

Enzyme and pathway databases

BioCyciSFUM335543:GH6P-1972-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit N 2UniRule annotation (EC:1.6.99.5UniRule annotation)
Alternative name(s):
NADH dehydrogenase I subunit N 2UniRule annotation
NDH-1 subunit N 2UniRule annotation
Gene namesi
Name:nuoN2UniRule annotation
Ordered Locus Names:Sfum_1935
OrganismiSyntrophobacter fumaroxidans (strain DSM 10017 / MPOB)
Taxonomic identifieri335543 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaSyntrophobacteralesSyntrophobacteraceaeSyntrophobacter
ProteomesiUP000001784: Chromosome

Subcellular locationi

Cell inner membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei3 – 2321HelicalUniRule annotationAdd
BLAST
Transmembranei34 – 5421HelicalUniRule annotationAdd
BLAST
Transmembranei67 – 8721HelicalUniRule annotationAdd
BLAST
Transmembranei106 – 12621HelicalUniRule annotationAdd
BLAST
Transmembranei156 – 17621HelicalUniRule annotationAdd
BLAST
Transmembranei198 – 21821HelicalUniRule annotationAdd
BLAST
Transmembranei233 – 25321HelicalUniRule annotationAdd
BLAST
Transmembranei263 – 28321HelicalUniRule annotationAdd
BLAST
Transmembranei291 – 31121HelicalUniRule annotationAdd
BLAST
Transmembranei317 – 33721HelicalUniRule annotationAdd
BLAST
Transmembranei360 – 38021HelicalUniRule annotationAdd
BLAST
Transmembranei398 – 41821HelicalUniRule annotationAdd
BLAST
Transmembranei441 – 46121HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 472472NADH-quinone oxidoreductase subunit N 2PRO_5000166272Add
BLAST

Keywords - PTMi

Quinone

Interactioni

Subunit structurei

NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.UniRule annotation

Protein-protein interaction databases

STRINGi335543.Sfum_1935.

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I subunit 2 family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1007.
HOGENOMiHOG000100794.
KOiK00343.
OMAiYLEHENG.
OrthoDBiEOG64JFNZ.

Family and domain databases

HAMAPiMF_00445. NDH1_NuoN_1.
InterProiIPR010096. NADH-Q_OxRdtase_suN/2.
IPR001750. NADH_UbQ/plastoQ_OxRdtase.
[Graphical view]
PfamiPF00361. Oxidored_q1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01770. NDH_I_N. 1 hit.

Sequencei

Sequence statusi: Complete.

A0LJL8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNWMSFAPEL ITLTSALWFL LLSMTARSDP KREHVAALVL SALGLAACLA
60 70 80 90 100
SVGAEGYCFA GAYKVDLFSQ VFKVLLAAGL FLIVTLCGEL SDIEERNRRE
110 120 130 140 150
FYVLLFVCTL AMMLLVGANH FLVVFISLEL SSYSLYVLVA LRRDRGLGLE
160 170 180 190 200
AGIKYFLVGI FASGVMIFGL ALLYGASGIA ALDGMARVLP GIIHQPAVVI
210 220 230 240 250
GLLLTLSGFF FKLAVFPFHF WAPDAYQGAA NQVSAYIATA SKVAAIGVLV
260 270 280 290 300
RVIASAGDGG TYLVHVLAVL SVVSMTVGNL AAIAQQDLKR LLAYSTVAHA
310 320 330 340 350
GYVLIGVLSM NPAGYSAAVF YAFALLVMKF TAFLVLVEVA SDGGNLRVEE
360 370 380 390 400
LAGLHRRSPI LALALMVSLF SLAGIPPTVG FTGKFLVFVA AMGEGHFTLV
410 420 430 440 450
LIAMINVVIS LYYYLLVIKA AYLLEPRQEL PALRVSPPLK LLSGVLVIAM
460 470
VAAGFFPNQI IRVAESAAKA LL
Length:472
Mass (Da):50,331
Last modified:December 12, 2006 - v1
Checksum:iA9F7BBF6C1DCCD73
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000478 Genomic DNA. Translation: ABK17620.1.
RefSeqiWP_011698790.1. NC_008554.1.
YP_846055.1. NC_008554.1.

Genome annotation databases

EnsemblBacteriaiABK17620; ABK17620; Sfum_1935.
GeneIDi4459740.
KEGGisfu:Sfum_1935.
PATRICi23849405. VBISynFum78438_2273.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000478 Genomic DNA. Translation: ABK17620.1.
RefSeqiWP_011698790.1. NC_008554.1.
YP_846055.1. NC_008554.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi335543.Sfum_1935.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK17620; ABK17620; Sfum_1935.
GeneIDi4459740.
KEGGisfu:Sfum_1935.
PATRICi23849405. VBISynFum78438_2273.

Phylogenomic databases

eggNOGiCOG1007.
HOGENOMiHOG000100794.
KOiK00343.
OMAiYLEHENG.
OrthoDBiEOG64JFNZ.

Enzyme and pathway databases

BioCyciSFUM335543:GH6P-1972-MONOMER.

Family and domain databases

HAMAPiMF_00445. NDH1_NuoN_1.
InterProiIPR010096. NADH-Q_OxRdtase_suN/2.
IPR001750. NADH_UbQ/plastoQ_OxRdtase.
[Graphical view]
PfamiPF00361. Oxidored_q1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01770. NDH_I_N. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 10017 / MPOB.

Entry informationi

Entry nameiNUON2_SYNFM
AccessioniPrimary (citable) accession number: A0LJL8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 9, 2010
Last sequence update: December 12, 2006
Last modified: February 4, 2015
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.