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Protein

Acetyl-coenzyme A synthetase

Gene

acsA

Organism
Syntrophobacter fumaroxidans (strain DSM 10017 / MPOB)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA.UniRule annotation

Catalytic activityi

ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei307 – 3071Coenzyme AUniRule annotation
Binding sitei331 – 3311Coenzyme AUniRule annotation
Binding sitei496 – 4961ATPUniRule annotation
Binding sitei511 – 5111ATPUniRule annotation
Binding sitei519 – 5191Coenzyme A; via carbonyl oxygenUniRule annotation
Binding sitei522 – 5221ATPUniRule annotation
Metal bindingi533 – 5331Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi535 – 5351Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi538 – 5381Magnesium; via carbonyl oxygenUniRule annotation
Binding sitei580 – 5801Coenzyme AUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi383 – 3853ATPUniRule annotation
Nucleotide bindingi407 – 4126ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSFUM335543:GH6P-762-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetyl-coenzyme A synthetaseUniRule annotation (EC:6.2.1.1UniRule annotation)
Short name:
AcCoA synthetaseUniRule annotation
Short name:
AcsUniRule annotation
Alternative name(s):
Acetate--CoA ligaseUniRule annotation
Acyl-activating enzymeUniRule annotation
Gene namesi
Name:acsAUniRule annotation
Ordered Locus Names:Sfum_0745
OrganismiSyntrophobacter fumaroxidans (strain DSM 10017 / MPOB)
Taxonomic identifieri335543 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaSyntrophobacteralesSyntrophobacteraceaeSyntrophobacter
Proteomesi
  • UP000001784 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 650650Acetyl-coenzyme A synthetasePRO_1000137278Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei605 – 6051N6-acetyllysineUniRule annotation

Post-translational modificationi

Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme.UniRule annotation

Keywords - PTMi

Acetylation

Interactioni

Protein-protein interaction databases

STRINGi335543.Sfum_0745.

Structurei

3D structure databases

ProteinModelPortaliA0LG91.
SMRiA0LG91. Positions 8-643.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni189 – 1924Coenzyme A bindingUniRule annotation

Sequence similaritiesi

Belongs to the ATP-dependent AMP-binding enzyme family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108IQF. Bacteria.
COG0365. LUCA.
HOGENOMiHOG000229981.
KOiK01895.
OMAiVMQVARV.
OrthoDBiPOG091H059D.

Family and domain databases

HAMAPiMF_01123. Ac_CoA_synth. 1 hit.
InterProiIPR011904. Ac_CoA_lig.
IPR032387. ACAS_N.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF16177. ACAS_N. 1 hit.
PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0LG91-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGNIFPVPE SWAKSAFCDN ETYLKMYEQS VKDPEGFWGE HAKRIDWFQP
60 70 80 90 100
WTKVKSGSFE GDVRFKWFEN GKLNVAYNCL DRNLAKRGDQ VAIIWEGDDP
110 120 130 140 150
KVSKYITYRE LHDQVCRFAN VLKAQGLKKG DRATIYLPMI PELAVAMLAC
160 170 180 190 200
ARIGVVHSIV FAGFSPESLA GRILDCGGKV VITADEGLRG GKPIPLKENT
210 220 230 240 250
EEALKKCPDV QKVIVVKHTG GKVPVVPGRD VDWTEAIKAA SPDCPPEVMD
260 270 280 290 300
AEDPLFILYT SGSTGKPKGV LHTTGGYLVY TALSHQYVFD YHDGDIYWCT
310 320 330 340 350
ADIGWVTGHS YIIYGPLANG ATTVMFEGIP NYPDWSRFWN VVDKHKINIF
360 370 380 390 400
YTAPTAIRAL MRQGEAPVRA TSRKSLKLLG TVGEPINPEA WLWYYNNVGE
410 420 430 440 450
QRCPIVDTWW QTETGGILIT PLPGATALKP GSATRPFFGV QPAIIDPEGK
460 470 480 490 500
MLDGPGSGYL IIKESWPGML RTVYGDHERF KQTYFSNYPG LYFTGDGARR
510 520 530 540 550
DEDGYYWITG RVDDVINVSG HRLGTAEVES ALVAHPAVAE SAVVGFPHDI
560 570 580 590 600
KGQGIYAYVT LKANWEHSDE LRQELVKWVR KEIGPIATPD YIQWAPGLPK
610 620 630 640 650
TRSGKIMRRI LRKIAANEID NLGDTTTLAE PAVVEDLIKN RQAVASSGKA
Length:650
Mass (Da):72,150
Last modified:December 12, 2006 - v1
Checksum:i3F2611C763B0E628
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000478 Genomic DNA. Translation: ABK16443.1.
RefSeqiWP_011697616.1. NC_008554.1.

Genome annotation databases

EnsemblBacteriaiABK16443; ABK16443; Sfum_0745.
KEGGisfu:Sfum_0745.
PATRICi23846559. VBISynFum78438_0869.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000478 Genomic DNA. Translation: ABK16443.1.
RefSeqiWP_011697616.1. NC_008554.1.

3D structure databases

ProteinModelPortaliA0LG91.
SMRiA0LG91. Positions 8-643.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi335543.Sfum_0745.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK16443; ABK16443; Sfum_0745.
KEGGisfu:Sfum_0745.
PATRICi23846559. VBISynFum78438_0869.

Phylogenomic databases

eggNOGiENOG4108IQF. Bacteria.
COG0365. LUCA.
HOGENOMiHOG000229981.
KOiK01895.
OMAiVMQVARV.
OrthoDBiPOG091H059D.

Enzyme and pathway databases

BioCyciSFUM335543:GH6P-762-MONOMER.

Family and domain databases

HAMAPiMF_01123. Ac_CoA_synth. 1 hit.
InterProiIPR011904. Ac_CoA_lig.
IPR032387. ACAS_N.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF16177. ACAS_N. 1 hit.
PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACSA_SYNFM
AccessioniPrimary (citable) accession number: A0LG91
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: December 12, 2006
Last modified: September 7, 2016
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.