A0LG91 (ACSA_SYNFM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetyl-coenzyme A synthetase
EC=6.2.1.1

Alternative name(s):
Acetate--CoA ligase
Acyl-activating enzyme

Gene names

Name:	acsA
Ordered Locus Names:	Sfum_0745

Organism

Syntrophobacter fumaroxidans (strain DSM 10017 / MPOB) [Complete proteome] [HAMAP]

Taxonomic identifier

335543 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Syntrophobacterales › Syntrophobacteraceae › Syntrophobacter

Protein attributes

Sequence length	650 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA. HAMAP MF_01123
Post-translational modification	Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme By similarity. HAMAP MF_01123
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	AMP binding Inferred from electronic annotation. Source: InterPro ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetate-CoA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 650	650	Acetyl-coenzyme A synthetase HAMAP MF_01123		PRO_1000137278
Sites
	Active site	513	1	By similarity
Amino acid modifications
	Modified residue	605	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0LG91 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 3F2611C763B0E628	FASTA	650	72,150
	10 20 30 40 50 60 MAGNIFPVPE SWAKSAFCDN ETYLKMYEQS VKDPEGFWGE HAKRIDWFQP WTKVKSGSFE 70 80 90 100 110 120 GDVRFKWFEN GKLNVAYNCL DRNLAKRGDQ VAIIWEGDDP KVSKYITYRE LHDQVCRFAN 130 140 150 160 170 180 VLKAQGLKKG DRATIYLPMI PELAVAMLAC ARIGVVHSIV FAGFSPESLA GRILDCGGKV 190 200 210 220 230 240 VITADEGLRG GKPIPLKENT EEALKKCPDV QKVIVVKHTG GKVPVVPGRD VDWTEAIKAA 250 260 270 280 290 300 SPDCPPEVMD AEDPLFILYT SGSTGKPKGV LHTTGGYLVY TALSHQYVFD YHDGDIYWCT 310 320 330 340 350 360 ADIGWVTGHS YIIYGPLANG ATTVMFEGIP NYPDWSRFWN VVDKHKINIF YTAPTAIRAL 370 380 390 400 410 420 MRQGEAPVRA TSRKSLKLLG TVGEPINPEA WLWYYNNVGE QRCPIVDTWW QTETGGILIT 430 440 450 460 470 480 PLPGATALKP GSATRPFFGV QPAIIDPEGK MLDGPGSGYL IIKESWPGML RTVYGDHERF 490 500 510 520 530 540 KQTYFSNYPG LYFTGDGARR DEDGYYWITG RVDDVINVSG HRLGTAEVES ALVAHPAVAE 550 560 570 580 590 600 SAVVGFPHDI KGQGIYAYVT LKANWEHSDE LRQELVKWVR KEIGPIATPD YIQWAPGLPK 610 620 630 640 650 TRSGKIMRRI LRKIAANEID NLGDTTTLAE PAVVEDLIKN RQAVASSGKA

References

[1]

"Complete sequence of Syntrophobacter fumaroxidans MPOB." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Goltsman E.G., Martinez M., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Boone D.R. , Brockman F., Culley D., Ferry J., Gunsalus R., McInerney M.J., Morrison M., Plugge C., Rohlin L., Scholten J., Sieber J., Stams A.J.M., Worm P., Henstra A.M., Richardson P. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 10017 / MPOB.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000478 Genomic DNA. Translation: ABK16443.1.
RefSeq	YP_844878.1. NC_008554.1.
3D structure databases
ProteinModelPortal	A0LG91.
SMR	A0LG91. Positions 8-643.
ModBase	Search...
Protein-protein interaction databases
STRING	A0LG91.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4460828.
GenomeReviews	Gene locus Sfum_0745 in contig CP000478_GR.
KEGG	sfu:Sfum_0745.
NMPDR	fig|335543.6.peg.775.
PATRIC	23846559. VBISynFum78438_0869.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0365.
HOGENOM	HBG547964.
OMA	WNCIERN.
PhylomeDB	A0LG91.
ProtClustDB	PRK00174.
Enzyme and pathway databases
BioCyc	SFUM335543:SFUM_0745-MONOMER.
Family and domain databases
HAMAP	MF_01123. Ac_CoA_synth. [Tree]
InterPro	IPR011904. Ac_CoA_lig. IPR024597. Acyl-CoA_synth_DUF3448. IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
KO	K01895.
PANTHER	PTHR24095:SF42. PTHR24095:SF42. 1 hit.
Pfam	PF00501. AMP-binding. 1 hit. PF11930. DUF3448. 1 hit. [Graphical view]
TIGRFAMs	TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACSA_SYNFM

Accession

Primary (citable) accession number: A0LG91

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	December 12, 2006
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program