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Protein

Malate dehydrogenase

Gene

mdh

Organism
Syntrophobacter fumaroxidans (strain DSM 10017 / MPOB)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate. Catalyzes the reduction of oxaloacetate more efficiently than the oxidation of malate.1 Publication

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation1 Publication

Enzyme regulationi

Substrate inhibition is observed at high concentrations of oxaloacetate.1 Publication

Kineticsi

  1. KM=50 µM for oxaloacetate (at 37 degrees Celsius and pH 7.5)1 Publication
  2. KM=30 µM for NADH (at 37 degrees Celsius and pH 7.5)1 Publication
  3. KM=4 mM for L-malate (at 37 degrees Celsius and pH 9.0)1 Publication
  4. KM=1.1 mM for NAD (at 37 degrees Celsius and pH 9.0)1 Publication

    pH dependencei

    Optimum pH is 8.5 with oxaloacetate as substrate.1 Publication

    Temperature dependencei

    Optimum temperature is 60 degrees Celsius with oxaloacetate as substrate.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei95SubstrateUniRule annotation1
    Binding sitei101SubstrateUniRule annotation1
    Binding sitei108NADUniRule annotation1
    Binding sitei115NADUniRule annotation1
    Binding sitei134SubstrateUniRule annotation1
    Binding sitei165SubstrateUniRule annotation1
    Active sitei190Proton acceptorUniRule annotation1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi12 – 18NADUniRule annotation7
    Nucleotide bindingi132 – 134NADUniRule annotation3

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionOxidoreductase
    Biological processTricarboxylic acid cycle
    LigandNAD

    Enzyme and pathway databases

    BioCyciSFUM335543:G1G7I-470-MONOMER

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
    Gene namesi
    Name:mdhUniRule annotation
    Ordered Locus Names:Sfum_0460
    OrganismiSyntrophobacter fumaroxidans (strain DSM 10017 / MPOB)
    Taxonomic identifieri335543 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaSyntrophobacteralesSyntrophobacteraceaeSyntrophobacter
    Proteomesi
    • UP000001784 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemoved1 Publication
    ChainiPRO_00002923762 – 329Malate dehydrogenaseAdd BLAST328

    Proteomic databases

    PRIDEiA0LFF8

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    STRINGi335543.Sfum_0460

    Structurei

    3D structure databases

    ProteinModelPortaliA0LFF8
    SMRiA0LFF8
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. MDH type 2 family.UniRule annotation

    Phylogenomic databases

    eggNOGiENOG4105D9Z Bacteria
    COG0039 LUCA
    HOGENOMiHOG000220953
    KOiK00024
    OMAiRPRTKGM
    OrthoDBiPOG091H03R4

    Family and domain databases

    Gene3Di3.90.110.101 hit
    HAMAPiMF_01517 Malate_dehydrog_2, 1 hit
    InterProiView protein in InterPro
    IPR001557 L-lactate/malate_DH
    IPR022383 Lactate/malate_DH_C
    IPR001236 Lactate/malate_DH_N
    IPR015955 Lactate_DH/Glyco_Ohase_4_C
    IPR010945 Malate_DH_type2
    IPR036291 NAD(P)-bd_dom_sf
    PANTHERiPTHR23382 PTHR23382, 1 hit
    PfamiView protein in Pfam
    PF02866 Ldh_1_C, 1 hit
    PF00056 Ldh_1_N, 1 hit
    PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
    SUPFAMiSSF51735 SSF51735, 1 hit
    SSF56327 SSF56327, 1 hit
    TIGRFAMsiTIGR01759 MalateDH-SF1, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A0LFF8-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MAKKPVRVTV TGAAGQIGYA LLFRVASGQM LGPDQPIILQ MLELPIDKVQ
    60 70 80 90 100
    AALKGVMMEL EDCAFPLLAD MIGTGDPKVA FKDSDYALLV GARPRGPGME
    110 120 130 140 150
    RKDLLLENAK IFIEQGKAMN AVASRDIRVI VVGNPANTNA WIAMKSAPDL
    160 170 180 190 200
    PKGNFTAMLR LDHNRAKSQL ATRTGKPVAS VEKMIVWGNH SPTMYPDIRF
    210 220 230 240 250
    CTVDGQPAVK LVNDEAWYRN EYIPKVGKRG AAIIEARGLS SAASAANAAI
    260 270 280 290 300
    DHMHDWALGT NGKWVTMGLP SDGSYGIPEG TMYGVPVTCT PGKYERVKGL
    310 320
    EIDAFSREKM DFTLKELTEE QAGVKEMVK
    Length:329
    Mass (Da):35,690
    Last modified:December 12, 2006 - v1
    Checksum:i1BE66E683446BE07
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CP000478 Genomic DNA Translation: ABK16160.1
    RefSeqiWP_011697333.1, NC_008554.1

    Genome annotation databases

    EnsemblBacteriaiABK16160; ABK16160; Sfum_0460
    KEGGisfu:Sfum_0460

    Similar proteinsi

    Entry informationi

    Entry nameiMDH_SYNFM
    AccessioniPrimary (citable) accession number: A0LFF8
    Secondary accession number(s): P80648
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 26, 2007
    Last sequence update: December 12, 2006
    Last modified: February 28, 2018
    This is version 76 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome