Reviewed, UniProtKB/Swiss-Prot A0L153 (KATG2_SHESA)

Names and origin

Protein names

Recommended name:
    Catalase-peroxidase 2
      Short name=CP 2
    EC=1.11.1.6
    EC=1.11.1.7
Alternative name(s):
    Peroxidase/catalase 2

Gene names

Name:	katG2
Ordered Locus Names:	Shewana3_3549

Organism

Shewanella sp. (strain ANA-3) [Complete proteome] [HAMAP]

Taxonomic identifier

94122 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	739 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity.
Catalytic activity	2 H2O2 = O2 + 2 H2O. HAMAP MF_01961 Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity.
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity.
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Domain	Signal
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: HAMAP heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 739	713	Catalase-peroxidase 2 HAMAP MF_01961		PRO_0000354924
Sites
	Active site	106	1	Proton acceptor By similarity
	Metal binding	268	1	Iron (heme axial ligand) By similarity
	Site	102	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	105 ↔ 227		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-253) By similarity
	Cross-link	227 ↔ 253		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-105) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0L153-1 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: D19AE6567BE114C6	FASTA	739	81,073
	10 20 30 40 50 60 MKKSTIPSMS ALTLAMSLAF GGAAIAQEQV TGNQFWWPEQ LNLSPLRQNA VESNPYGSDY 70 80 90 100 110 120 HYAEAFKSLD LDAVKKDIKA LMTESQDWWP SDYGHYGPFF IRMAWHSAGV YRIFDGRGGA 130 140 150 160 170 180 AGGQQRFEPL NSWPDNVNLD KARRLLWPIK QKYGSKISWG DLMVLTGNVA LESMGFKTFG 190 200 210 220 230 240 FAGGRVDDWE AEQVNWGSEK AWLDSKRRNE KGELAKPMGA TQMGLIYVNP EGPNGVPDPL 250 260 270 280 290 300 ASAKEIRDTF GRMAMNDEET VALIAGGHTF GKAHGAHDPS KCVGADPAAS GVEAQGLGWK 310 320 330 340 350 360 NKCGKGHSED TVTSGLEGAW SVNPTAWTMQ YLENLYGFDW VQTKSPAGHI QWIPKDGKGA 370 380 390 400 410 420 NLVPDAHDKS KRHAPIMFTS DIALKEDPSY REITTRFLKN PKEFELAFAK AWFKLTHRDM 430 440 450 460 470 480 GPKARYLGAD VPAEMLIWQD PIPALDHPVI DNADIKALGN KILASGLTVP ELVRTAWASA 490 500 510 520 530 540 SSFRGTDMRG GANGARIRLE PMMNWQANNP KELAKVLAKL EKVQKDVNSS LKGGKKVSLA 550 560 570 580 590 600 DVIVLGGSVA VEKAAKEAGV TVSVPFTPGR MDATQAQTDV SSFAVLEPTA DGFRNYYSKD 610 620 630 640 650 660 SSHSPAEMLI ERANMLNLTV PEMTVLVGGL RALDANSAGV KHGVFTDKPG TLSNDFFVNL 670 680 690 700 710 720 LDMSTKWSKS EKQEGIYEGQ DRKSGKLKWT ATPVDLVFGS HSELRAVSEV YGAQDGQDRF 730 VQDFIKAWNK VMNADRFDI

References

[1]

"Complete sequence of chromosome 1 of Shewanella sp. ANA-3." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Newman D., Salticov C., Konstantinidis K., Klappenback J., Tiedje J., Richardson P. Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000469 Genomic DNA. Translation: ABK49772.1.
RefSeq	YP_871178.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A0L153.
Genome annotation databases
GeneID	4476149.
GenomeReviews	Gene locus Shewana3_3549 in contig CP000469_GR.
KEGG	shn:Shewana3_3549.
NMPDR	fig|94122.5.peg.3544.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	GTDMRGG.
Family and domain databases
HAMAP	MF_01961. [Tree]
InterPro	IPR000763. Catalase_proxase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
TIGRFAMs	TIGR00198. cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG2_SHESA

Accession

Primary (citable) accession number: A0L153

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	December 12, 2006
Last modified:	November 3, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)