Reviewed, UniProtKB/Swiss-Prot A0KYF8 (RIBA_SHESA)

Names and origin

Protein names

Recommended name:
    GTP cyclohydrolase-2
    EC=3.5.4.25
Alternative name(s):
    GTP cyclohydrolase II

Gene names

Name:	ribA
Ordered Locus Names:	Shewana3_2600

Organism

Shewanella sp. (strain ANA-3) [Complete proteome] [HAMAP]

Taxonomic identifier

94122 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	203 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate By similarity.
Catalytic activity	GTP + 3 H2O = formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine + diphosphate. HAMAP MF_00179
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; 6,7-dimethyl-8-(1-D-ribityl)lumazine from GTP: step 1/4. HAMAP MF_00179
Sequence similarities	Belongs to the GTP cyclohydrolase II family.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Ligand	GTP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP cyclohydrolase II activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 203	203	GTP cyclohydrolase-2 HAMAP MF_00179		PRO_1000040585
Regions
	Nucleotide binding	49 – 53	5	GTP By similarity
	Nucleotide binding	92 – 94	3	GTP By similarity
Sites
	Active site	126	1	Proton acceptor Potential
	Active site	128	1	Nucleophile By similarity
	Metal binding	54	1	Zinc; catalytic By similarity
	Metal binding	65	1	Zinc; catalytic By similarity
	Metal binding	67	1	Zinc; catalytic By similarity
	Binding site	70	1	GTP By similarity
	Binding site	114	1	GTP By similarity
	Binding site	149	1	GTP By similarity
	Binding site	154	1	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0KYF8-1 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 64A796ECC4C79BBC	FASTA	203	22,838
	10 20 30 40 50 60 MSIKYVATSK LPTPWGVFAM HGFEDTETSK EHVALTFGTL SSDAPVLGRI HSECLTGDAL 70 80 90 100 110 120 FSLRCDCGFQ LQTAMQNIAE TGSGFILYLR QEGRGIGLLN KIRAYELQDK GANTVEANEQ 130 140 150 160 170 180 LGFPADMRKY DMIQPMLEQI GVKHVRLMTN NPRKVKAMKE IGIEVVERVP LQVGKNRYNE 190 200 AYLKTKSTEL GHMMSEYHFT DEE

References

[1]

"Complete sequence of chromosome 1 of Shewanella sp. ANA-3." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Newman D., Salticov C., Konstantinidis K., Klappenback J., Tiedje J., Richardson P. Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000469 Genomic DNA. Translation: ABK48827.1.
RefSeq	YP_870233.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4478468.
GenomeReviews	Gene locus Shewana3_2600 in contig CP000469_GR.
KEGG	shn:Shewana3_2600.
NMPDR	fig|94122.5.peg.2644.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A0KYF8. QGFILYL.
Family and domain databases
HAMAP	MF_00179. [Tree]
InterPro	IPR000926. GTP_CycHdrlase_II. [Graphical view]
Pfam	PF00925. GTP_cyclohydro2. 1 hit. [Graphical view]
TIGRFAMs	TIGR00505. ribA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RIBA_SHESA

Accession

Primary (citable) accession number: A0KYF8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	December 12, 2006
Last modified:	June 16, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)