ID   ASTE_SHESA              Reviewed;         344 AA.
AC   A0KX43;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   12-DEC-2006, sequence version 1.
DT   16-JUN-2009, entry version 20.
DE   RecName: Full=Succinylglutamate desuccinylase;
DE            EC=3.5.1.96;
GN   Name=astE; OrderedLocusNames=Shewana3_2132;
OS   Shewanella sp. (strain ANA-3).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC   Shewanellaceae; Shewanella.
OX   NCBI_TaxID=94122;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H.,
RA   Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R.,
RA   Gilna P., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA   Kim E., Newman D., Salticov C., Konstantinidis K., Klappenback J.,
RA   Tiedje J., Richardson P.;
RT   "Complete sequence of chromosome 1 of Shewanella sp. ANA-3.";
RL   Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Transforms N(2)-succinylglutamate into succinate and
CC       glutamate (By similarity).
CC   -!- CATALYTIC ACTIVITY: N-succinyl-L-glutamate + H(2)O = succinate +
CC       L-glutamate.
CC   -!- COFACTOR: Binds 1 zinc ion per subunit (By similarity).
CC   -!- PATHWAY: Amino-acid degradation; L-arginine degradation via AST
CC       pathway; L-glutamate and succinate from L-arginine: step 5/5.
CC   -!- SIMILARITY: Belongs to the aspA/astE family. Succinylglutamate
CC       desuccinylase subfamily.
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DR   EMBL; CP000469; ABK48362.1; -; Genomic_DNA.
DR   RefSeq; YP_869768.1; -.
DR   GeneID; 4478328; -.
DR   GenomeReviews; CP000469_GR; Shewana3_2132.
DR   KEGG; shn:Shewana3_2132; -.
DR   NMPDR; fig|94122.5.peg.2197; -.
DR   OMA; A0KX43; EKFAIYP.
DR   GO; GO:0016788; F:hydrolase activity, acting on ester bonds; IEA:HAMAP.
DR   GO; GO:0009017; F:succinylglutamate desuccinylase activity; IEA:EC.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0019544; P:arginine catabolic process to glutamate; IEA:HAMAP.
DR   HAMAP; MF_00767; -; 1.
DR   InterPro; IPR007036; Aste_AspA.
DR   InterPro; IPR016681; SuccinylGlu_desuccinylase.
DR   Pfam; PF04952; AstE_AspA; 1.
DR   PIRSF; PIRSF017020; AstE; 1.
DR   TIGRFAMs; TIGR03242; arg_catab_astE; 1.
PE   3: Inferred from homology;
KW   Arginine metabolism; Complete proteome; Hydrolase; Metal-binding;
KW   Zinc.
FT   CHAIN         1    344       Succinylglutamate desuccinylase.
FT                                /FTId=PRO_1000017330.
FT   ACT_SITE    224    224       Potential.
FT   METAL        63     63       Zinc (By similarity).
FT   METAL        66     66       Zinc (By similarity).
FT   METAL       160    160       Zinc (By similarity).
SQ   SEQUENCE   344 AA;  38685 MW;  192B8A4CC60B0D05 CRC64;
     MLQALLDSKD FLALTLANPE TLGDEFSFTL GEHTRVEVWN TGVIVFEPAQ AQGKDVILSC
     GVHGNETAPI ELCNTLIKQL LQQKIIAKQR TLFLIGNPLA INNGTRIIDE NMNRLFSGEH
     SNPPGLVNPE RVRAKKLEAY VDRFFKGAAA GRQRIHYDLH TAMRASKHEK FAIYPYRPGR
     AYSAEQIMFL AASGVDTVLF HHEPTTTFSY FSSEQYGADA FTIELGKVYP MGQNDMTRFI
     AAQEMFMRLI TDKPLALEPF SADKVNLYQV CRVINKHFDD FEFTFAADVE NFRSFPKGFV
     LAREGGQEIK VEQEFEAIVF PNAKVPIGNR TVICLIPSVA PDVR
//