A0KX43 (ASTE_SHESA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Succinylglutamate desuccinylase
EC=3.5.1.96

Gene names

Name:	astE
Ordered Locus Names:	Shewana3_2132

Organism

Shewanella sp. (strain ANA-3) [Complete proteome] [HAMAP]

Taxonomic identifier

94122 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Transforms N(2)-succinylglutamate into succinate and glutamate By similarity. HAMAP MF_00767
Catalytic activity	N-succinyl-L-glutamate + H2O = succinate + L-glutamate. HAMAP MF_00767
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00767
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 5/5. HAMAP MF_00767
Sequence similarities	Belongs to the AspA/AstE family. Succinylglutamate desuccinylase subfamily.

Ontologies

Keywords
Biological process	Arginine metabolism
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine catabolic process to glutamate Inferred from electronic annotation. Source: InterPro
Molecular function	hydrolase activity, acting on ester bonds Inferred from electronic annotation. Source: InterPro succinylglutamate desuccinylase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	Succinylglutamate desuccinylase HAMAP MF_00767		PRO_1000017330
Sites
	Active site	224	1	Potential
	Metal binding	63	1	Zinc By similarity
	Metal binding	66	1	Zinc By similarity
	Metal binding	160	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0KX43 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 192B8A4CC60B0D05	FASTA	344	38,685
	10 20 30 40 50 60 MLQALLDSKD FLALTLANPE TLGDEFSFTL GEHTRVEVWN TGVIVFEPAQ AQGKDVILSC 70 80 90 100 110 120 GVHGNETAPI ELCNTLIKQL LQQKIIAKQR TLFLIGNPLA INNGTRIIDE NMNRLFSGEH 130 140 150 160 170 180 SNPPGLVNPE RVRAKKLEAY VDRFFKGAAA GRQRIHYDLH TAMRASKHEK FAIYPYRPGR 190 200 210 220 230 240 AYSAEQIMFL AASGVDTVLF HHEPTTTFSY FSSEQYGADA FTIELGKVYP MGQNDMTRFI 250 260 270 280 290 300 AAQEMFMRLI TDKPLALEPF SADKVNLYQV CRVINKHFDD FEFTFAADVE NFRSFPKGFV 310 320 330 340 LAREGGQEIK VEQEFEAIVF PNAKVPIGNR TVICLIPSVA PDVR

References

[1]

"Complete sequence of chromosome 1 of Shewanella sp. ANA-3." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Newman D., Salticov C., Konstantinidis K., Klappenback J., Tiedje J., Richardson P. Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ANA-3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000469 Genomic DNA. Translation: ABK48362.1.
RefSeq	YP_869768.1. NC_008577.1.
3D structure databases
ProteinModelPortal	A0KX43.
ModBase	Search...
Protein-protein interaction databases
STRING	A0KX43.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4478328.
GenomeReviews	Gene locus Shewana3_2132 in contig CP000469_GR.
KEGG	shn:Shewana3_2132.
NMPDR	fig|94122.5.peg.2197.
PATRIC	23572103. VBISheSp134792_2446.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2988.
HOGENOM	HBG298271.
OMA	RWRWLGH.
PhylomeDB	A0KX43.
ProtClustDB	PRK05324.
Enzyme and pathway databases
BioCyc	SSP94122:SHEWANA3_2132-MONOMER.
Family and domain databases
HAMAP	MF_00767. Arg_catab_AstE. [Tree]
InterPro	IPR007036. Aste_AspA. IPR016681. SuccinylGlu_desuccinylase. [Graphical view]
KO	K05526.
Pfam	PF04952. AstE_AspA. 1 hit. [Graphical view]
PIRSF	PIRSF017020. AstE. 1 hit.
TIGRFAMs	TIGR03242. Arg_catab_astE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ASTE_SHESA

Accession

Primary (citable) accession number: A0KX43

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	December 12, 2006
Last modified:	December 14, 2011
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program