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Protein

3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase

Gene

fabA

Organism
Shewanella sp. (strain ANA-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Necessary for the introduction of cis unsaturation into fatty acids. Catalyzes the dehydration of (3R)-3-hydroxydecanoyl-ACP to E-(2)-decenoyl-ACP and then its isomerization to Z-(3)-decenoyl-ACP. Can catalyze the dehydratase reaction for beta-hydroxyacyl-ACPs with saturated chain lengths up to 16:0, being most active on intermediate chain length.UniRule annotation

Catalytic activityi

A (3R)-3-hydroxyacyl-[acyl-carrier protein] = a trans-2-enoyl-[acyl-carrier protein] + H2O.UniRule annotation
(3R)-3-hydroxydecanoyl-[acyl-carrier-protein] = trans-dec-2-enoyl-[acyl-carrier-protein] + H2O.UniRule annotation
Trans-dec-2-enoyl-[acyl-carrier-protein] = cis-dec-3-enoyl-[acyl-carrier-protein].UniRule annotation

Pathwayi: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei70 – 701UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Lyase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciSSP94122:GJ9K-1678-MONOMER.
UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
3-hydroxydecanoyl-[acyl-carrier-protein] dehydrataseUniRule annotation (EC:4.2.1.59UniRule annotation)
Alternative name(s):
3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabAUniRule annotation
Beta-hydroxydecanoyl thioester dehydraseUniRule annotation
Trans-2-decenoyl-[acyl-carrier-protein] isomeraseUniRule annotation (EC:5.3.3.14UniRule annotation)
Gene namesi
Name:fabAUniRule annotation
Ordered Locus Names:Shewana3_1606
OrganismiShewanella sp. (strain ANA-3)
Taxonomic identifieri94122 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
Proteomesi
  • UP000002589 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 1711713-hydroxydecanoyl-[acyl-carrier-protein] dehydratasePRO_1000201218Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi94122.Shewana3_1606.

Structurei

3D structure databases

ProteinModelPortaliA0KVM1.
SMRiA0KVM1. Positions 1-171.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thioester dehydratase family. FabA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108RKI. Bacteria.
COG0764. LUCA.
HOGENOMiHOG000277828.
KOiK01716.
OMAiFFDCHFK.
OrthoDBiEOG6VB6SH.

Family and domain databases

Gene3Di3.10.129.10. 1 hit.
HAMAPiMF_00405. FabA.
InterProiIPR010083. FabA.
IPR013114. FabA_FabZ.
IPR029069. HotDog_dom.
[Graphical view]
PfamiPF07977. FabA. 1 hit.
[Graphical view]
SUPFAMiSSF54637. SSF54637. 1 hit.
TIGRFAMsiTIGR01749. fabA. 1 hit.

Sequencei

Sequence statusi: Complete.

A0KVM1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKANSFNKE ELIACGHGNL FGPNSPRLPV DNMLMIDRII TINDNGGEFG
60 70 80 90 100
KGEIVAELDI NPDLWFFGCH FISDPVMPGC LGLDAMWQLV GFYLGWEGAE
110 120 130 140 150
GKGRALGVGE VKFTGQVLPG AKKVTYKLNI KRTIHRKLVM GIADAILEVD
160 170
GRQIYSATDL KVGVFSDTST F
Length:171
Mass (Da):18,694
Last modified:December 12, 2006 - v1
Checksum:iE882032D9DB7D3AD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000469 Genomic DNA. Translation: ABK47840.1.
RefSeqiWP_011625892.1. NC_008577.1.

Genome annotation databases

EnsemblBacteriaiABK47840; ABK47840; Shewana3_1606.
KEGGishn:Shewana3_1606.
PATRICi23570966. VBISheSp134792_1892.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000469 Genomic DNA. Translation: ABK47840.1.
RefSeqiWP_011625892.1. NC_008577.1.

3D structure databases

ProteinModelPortaliA0KVM1.
SMRiA0KVM1. Positions 1-171.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi94122.Shewana3_1606.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK47840; ABK47840; Shewana3_1606.
KEGGishn:Shewana3_1606.
PATRICi23570966. VBISheSp134792_1892.

Phylogenomic databases

eggNOGiENOG4108RKI. Bacteria.
COG0764. LUCA.
HOGENOMiHOG000277828.
KOiK01716.
OMAiFFDCHFK.
OrthoDBiEOG6VB6SH.

Enzyme and pathway databases

UniPathwayiUPA00094.
BioCyciSSP94122:GJ9K-1678-MONOMER.

Family and domain databases

Gene3Di3.10.129.10. 1 hit.
HAMAPiMF_00405. FabA.
InterProiIPR010083. FabA.
IPR013114. FabA_FabZ.
IPR029069. HotDog_dom.
[Graphical view]
PfamiPF07977. FabA. 1 hit.
[Graphical view]
SUPFAMiSSF54637. SSF54637. 1 hit.
TIGRFAMsiTIGR01749. fabA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ANA-3.

Entry informationi

Entry nameiFABA_SHESA
AccessioniPrimary (citable) accession number: A0KVM1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: December 12, 2006
Last modified: November 11, 2015
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.