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Protein

Enolase

Gene

eno

Organism
Shewanella sp. (strain ANA-3)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.UniRule annotation

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI)
  4. Enolase (eno)
  5. Pyruvate kinase (Shewana3_2153)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei159SubstrateUniRule annotation1
Binding sitei168SubstrateUniRule annotation1
Active sitei209Proton donorUniRule annotation1
Metal bindingi246MagnesiumUniRule annotation1
Metal bindingi289MagnesiumUniRule annotation1
Binding sitei289SubstrateUniRule annotation1
Metal bindingi316MagnesiumUniRule annotation1
Binding sitei316SubstrateUniRule annotation1
Active sitei341Proton acceptorUniRule annotation1
Binding sitei341Substrate (covalent); in inhibited formUniRule annotation1
Binding sitei392SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187

Names & Taxonomyi

Protein namesi
Recommended name:
EnolaseUniRule annotation (EC:4.2.1.11UniRule annotation)
Alternative name(s):
2-phospho-D-glycerate hydro-lyaseUniRule annotation
2-phosphoglycerate dehydrataseUniRule annotation
Gene namesi
Name:enoUniRule annotation
Ordered Locus Names:Shewana3_1116
OrganismiShewanella sp. (strain ANA-3)
Taxonomic identifieri94122 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
Proteomesi
  • UP000002589 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Secreted UniRule annotation
  • Cell surface UniRule annotation
  • Note: Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002808751 – 431EnolaseAdd BLAST431

Interactioni

Subunit structurei

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation.UniRule annotation

Protein-protein interaction databases

STRINGi94122.Shewana3_1116

Structurei

3D structure databases

ProteinModelPortaliA0KU82
SMRiA0KU82
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni368 – 371Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the enolase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C70 Bacteria
COG0148 LUCA
HOGENOMiHOG000072174
KOiK01689
OMAiEFMIIPV
OrthoDBiPOG091H02DK

Family and domain databases

CDDicd03313 enolase, 1 hit
Gene3Di3.20.20.1201 hit
3.30.390.101 hit
HAMAPiMF_00318 Enolase, 1 hit
InterProiView protein in InterPro
IPR000941 Enolase
IPR036849 Enolase-like_C_sf
IPR029017 Enolase-like_N
IPR034390 Enolase-like_superfamily
IPR020810 Enolase_C
IPR020809 Enolase_CS
IPR020811 Enolase_N
PANTHERiPTHR11902 PTHR11902, 1 hit
PfamiView protein in Pfam
PF00113 Enolase_C, 1 hit
PF03952 Enolase_N, 1 hit
PIRSFiPIRSF001400 Enolase, 1 hit
PRINTSiPR00148 ENOLASE
SFLDiSFLDG00178 enolase, 1 hit
SFLDS00001 Enolase, 1 hit
SMARTiView protein in SMART
SM01192 Enolase_C, 1 hit
SM01193 Enolase_N, 1 hit
SUPFAMiSSF51604 SSF51604, 1 hit
TIGRFAMsiTIGR01060 eno, 1 hit
PROSITEiView protein in PROSITE
PS00164 ENOLASE, 1 hit

Sequencei

Sequence statusi: Complete.

A0KU82-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKIINVIGR EIMDSRGNPT VEAEVHLEGG FIGMAAAPSG ASTGSREALE
60 70 80 90 100
LRDGDKSRYL GKGVLTAVAN VNGPIRAALI GKDATAQAEL DQIMIDLDGT
110 120 130 140 150
ENKDKLGANA ILAVSLAAAK AAAAFKGMPL YAHIAELNGT PGQYAMPVPM
160 170 180 190 200
MNILNGGEHA DNNVDIQEFM VQPVGAKNFR EALRMGAEIF HTLKKVLHGK
210 220 230 240 250
GLSTSVGDEG GFAPNLSSNA DALAVIKEAV ELAGYKLGTD VTLALDCAAS
260 270 280 290 300
EFYKDGKYDL SGEGKVFDSN GFSDFLKSLT EQYPIVSIED GLDESDWDGW
310 320 330 340 350
AYQTKIMGDK IQLVGDDLFV TNTKILTRGI ENGIANSILI KFNQIGSLTE
360 370 380 390 400
TLAAIRMAKA AGYTAVISHR SGETEDSTIA DLAVGTAAGQ IKTGSLCRSD
410 420 430
RVAKYNQLLR IEEQLGEKAP YRGLKEIKGQ A
Length:431
Mass (Da):45,689
Last modified:December 12, 2006 - v1
Checksum:i937C124EB70CC7BF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000469 Genomic DNA Translation: ABK47351.1
RefSeqiWP_011716218.1, NC_008577.1

Genome annotation databases

EnsemblBacteriaiABK47351; ABK47351; Shewana3_1116
KEGGishn:Shewana3_1116

Similar proteinsi

Entry informationi

Entry nameiENO_SHESA
AccessioniPrimary (citable) accession number: A0KU82
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: December 12, 2006
Last modified: February 28, 2018
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome