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A0KU01

- PNP_SHESA

UniProt

A0KU01 - PNP_SHESA

Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Shewanella sp. (strain ANA-3)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 59 (01 Oct 2014)
      Sequence version 1 (12 Dec 2006)
      Previous versions | rss
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    Functioni

    Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

    Catalytic activityi

    RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi485 – 4851MagnesiumUniRule annotation
    Metal bindingi491 – 4911MagnesiumUniRule annotation

    GO - Molecular functioni

    1. 3'-5'-exoribonuclease activity Source: InterPro
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
    4. RNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. mRNA catabolic process Source: UniProtKB-HAMAP
    2. RNA processing Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium, Metal-binding, RNA-binding

    Enzyme and pathway databases

    BioCyciSSP94122:GJ9K-1071-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
    Alternative name(s):
    Polynucleotide phosphorylaseUniRule annotation
    Short name:
    PNPaseUniRule annotation
    Gene namesi
    Name:pnpUniRule annotation
    Ordered Locus Names:Shewana3_1035
    OrganismiShewanella sp. (strain ANA-3)
    Taxonomic identifieri94122 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
    ProteomesiUP000002589: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 699699Polyribonucleotide nucleotidyltransferasePRO_0000329844Add
    BLAST

    Interactioni

    Subunit structurei

    Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation.UniRule annotation

    Protein-protein interaction databases

    STRINGi94122.Shewana3_1035.

    Structurei

    3D structure databases

    ProteinModelPortaliA0KU01.
    SMRiA0KU01. Positions 616-691.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini552 – 61160KHUniRule annotationAdd
    BLAST
    Domaini621 – 68969S1 motifUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
    Contains 1 KH domain.UniRule annotation
    Contains 1 S1 motif domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1185.
    HOGENOMiHOG000218326.
    KOiK00962.
    OMAiPRWDWVA.
    OrthoDBiEOG6WT8CC.

    Family and domain databases

    Gene3Di1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPiMF_01595. PNPase.
    InterProiIPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view]
    PANTHERiPTHR11252. PTHR11252. 1 hit.
    PfamiPF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005499. PNPase. 1 hit.
    SMARTiSM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view]
    SUPFAMiSSF46915. SSF46915. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
    PROSITEiPS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A0KU01-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNPIVKSFEY GQHTVTLETG VIARQADAAV LASMGDTTVL VTVVGKKEAE    50
    AGRDFFPLTV NYQEKTYAAG KIPGGFFKRE GRPSEDETLI ARLIDRPIRP 100
    LFPNGFTNEV QVIITVVSVD PQIEPDIISM IGTSAALAIS GIPFSGPLGA 150
    ARVGYINGEY VLNPTVTQLE SSQLNLVVAG TESAVLMVES EAQALPEEVM 200
    LGSVVYGHDQ QQVVIKAIAE FKAEAGKPAW NWTAPVANEA LVAQVKELAE 250
    DGLAQAYQIQ VKQDRYAQVA VVKAAAKEAL LAANPDVDLR EVDGLLGSLE 300
    KKVVRGRIIR GEPRIDGREP DMVRALSVLA GVLPRTHGSA LFTRGETQAL 350
    VTCTLGTERD AQKIDSIMGE RTNRFMLHYN FPPYSVGETG MVGSPKRREI 400
    GHGKLAWRGI NAVMPTAEEF PYSVRVVSEI TESNGSSSMA SVCGTSLALM 450
    DAGVPIKTSV AGIAMGLVKE GDNFVVLSDI LGDEDHLGDM DFKVAGTRDG 500
    VTALQMDIKI EGITKEIMEI ALQQAYGARV HILNVMDQAI GSHRDDISDH 550
    APRITTIKIN PEKIRDVIGK GGAVIRALTE ETGTTIELED DGTVKIASSN 600
    GDATREAIRR IEEITSEVEV GRIYNGKVIR IVDFGAFVNI LPGKDGLVHI 650
    SQISDERVAN VSDHLELNQE VTVKVMEVDR QGRVRLSIKE AQTKEAAAE 699
    Length:699
    Mass (Da):75,365
    Last modified:December 12, 2006 - v1
    Checksum:i7B11EBF180317C9D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000469 Genomic DNA. Translation: ABK47270.1.
    RefSeqiWP_011716147.1. NC_008577.1.
    YP_868676.1. NC_008577.1.

    Genome annotation databases

    EnsemblBacteriaiABK47270; ABK47270; Shewana3_1035.
    GeneIDi4479380.
    KEGGishn:Shewana3_1035.
    PATRICi23569708. VBISheSp134792_1299.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000469 Genomic DNA. Translation: ABK47270.1 .
    RefSeqi WP_011716147.1. NC_008577.1.
    YP_868676.1. NC_008577.1.

    3D structure databases

    ProteinModelPortali A0KU01.
    SMRi A0KU01. Positions 616-691.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 94122.Shewana3_1035.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABK47270 ; ABK47270 ; Shewana3_1035 .
    GeneIDi 4479380.
    KEGGi shn:Shewana3_1035.
    PATRICi 23569708. VBISheSp134792_1299.

    Phylogenomic databases

    eggNOGi COG1185.
    HOGENOMi HOG000218326.
    KOi K00962.
    OMAi PRWDWVA.
    OrthoDBi EOG6WT8CC.

    Enzyme and pathway databases

    BioCyci SSP94122:GJ9K-1071-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPi MF_01595. PNPase.
    InterProi IPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view ]
    PANTHERi PTHR11252. PTHR11252. 1 hit.
    Pfami PF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005499. PNPase. 1 hit.
    SMARTi SM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46915. SSF46915. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
    PROSITEi PS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ANA-3.

    Entry informationi

    Entry nameiPNP_SHESA
    AccessioniPrimary (citable) accession number: A0KU01
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: December 12, 2006
    Last modified: October 1, 2014
    This is version 59 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3