A0KT85 (ISPE_SHESA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	Shewana3_0766

Organism

Shewanella sp. (strain ANA-3) [Complete proteome] [HAMAP]

Taxonomic identifier

94122 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP-Rule MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP-Rule MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP-Rule MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: UniProtKB-UniPathway terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP-Rule MF_00061		PRO_1000007891
Regions
	Nucleotide binding	98 – 108	11	ATP Potential
Sites
	Active site	14	1	By similarity
	Active site	140	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0KT85 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: CD601944B01FF197	FASTA	284	30,766
	10 20 30 40 50 60 MSNEISRNWP APAKLNLFLH INGRRADGYH ELQTLFQFID CCDQLDFRVT QTPELILHSN 70 80 90 100 110 120 MSAVVADSDN LILRAAKSLQ QATSYPGGAE IWLEKRLPMG GGLGGGSSDA ATTLVALNQL 130 140 150 160 170 180 WNTQLSNDEL AAIGLKLGAD IPVFIRGFAA FAEGVGERLQ AVTPTEFWYL VIAPDAHVST 190 200 210 220 230 240 AAVFQDPLLP RNTPKLGIDT LMSQPWANDC QDLVVSKYPQ VAKALGWLLE YAPSRMTGTG 250 260 270 280 ACVFGEFSSQ QQALAALAKL PSDMQGFVAK GMNISPLIVR LTHP

References

[1]

"Complete sequence of chromosome 1 of Shewanella sp. ANA-3." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Newman D., Salticov C., Konstantinidis K., Klappenback J., Tiedje J., Richardson P. Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ANA-3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000469 Genomic DNA. Translation: ABK47004.1.
RefSeq	YP_868410.1. NC_008577.1.
3D structure databases
ProteinModelPortal	A0KT85.
SMR	A0KT85. Positions 6-281.
ModBase	Search...
Protein-protein interaction databases
STRING	94122.Shewana3_0766.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABK47004; ABK47004; Shewana3_0766.
GeneID	4476977.
KEGG	shn:Shewana3_0766.
PATRIC	23569130. VBISheSp134792_1020.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1947.
HOGENOM	HOG000019601.
KO	K00919.
OMA	TGACVFG.
ProtClustDB	PRK00343.
Enzyme and pathway databases
UniPathway	UPA00056; UER00094.
Family and domain databases
Gene3D	3.30.230.10. 1 hit.
HAMAP	MF_00061. IspE.
InterPro	IPR013750. GHMP_kinase_C_dom. IPR006204. GHMP_kinase_N_dom. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
PANTHER	PTHR20861:SF2. PTHR20861:SF2. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. ispE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_SHESA

Accession

Primary (citable) accession number: A0KT85

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	December 12, 2006
Last modified:	May 1, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program