ID   MAO1_SHESA              Reviewed;         562 AA.
AC   A0KT69;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   12-DEC-2006, sequence version 1.
DT   16-JUN-2009, entry version 23.
DE   RecName: Full=NAD-dependent malic enzyme;
DE            Short=NAD-ME;
DE            EC=1.1.1.38;
GN   Name=sfcA; OrderedLocusNames=Shewana3_0750;
OS   Shewanella sp. (strain ANA-3).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC   Shewanellaceae; Shewanella.
OX   NCBI_TaxID=94122;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H.,
RA   Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R.,
RA   Gilna P., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA   Kim E., Newman D., Salticov C., Konstantinidis K., Klappenback J.,
RA   Tiedje J., Richardson P.;
RT   "Complete sequence of chromosome 1 of Shewanella sp. ANA-3.";
RL   Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: (S)-malate + NAD(+) = pyruvate + CO(2) + NADH.
CC   -!- COFACTOR: Divalent metal cations. Prefers magnesium or manganese
CC       (By similarity).
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SIMILARITY: Belongs to the malic enzymes family.
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DR   EMBL; CP000469; ABK46988.1; -; Genomic_DNA.
DR   RefSeq; YP_868394.1; -.
DR   GeneID; 4476961; -.
DR   GenomeReviews; CP000469_GR; Shewana3_0750.
DR   KEGG; shn:Shewana3_0750; -.
DR   NMPDR; fig|94122.5.peg.877; -.
DR   OMA; A0KT69; IQDNNET.
DR   GO; GO:0016619; F:malate dehydrogenase (oxaloacetate-decarbox...; IEA:HAMAP.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0051287; F:NAD or NADH binding; IEA:InterPro.
DR   GO; GO:0006108; P:malate metabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_01619; -; 1.
DR   InterPro; IPR015884; Malic_enzyme_CS.
DR   InterPro; IPR012301; Malic_N.
DR   InterPro; IPR012302; Malic_NAD_bd.
DR   InterPro; IPR001891; Malic_OxRdtase.
DR   Pfam; PF00390; malic; 1.
DR   Pfam; PF03949; Malic_M; 1.
DR   PRINTS; PR00072; MALOXRDTASE.
DR   PROSITE; PS00331; MALIC_ENZYMES; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Metal-binding; NAD; Oxidoreductase.
FT   CHAIN         1    562       NAD-dependent malic enzyme.
FT                                /FTId=PRO_1000069545.
FT   ACT_SITE    101    101       Proton donor (By similarity).
FT   ACT_SITE    172    172       Proton acceptor (By similarity).
FT   METAL       243    243       Divalent metal cation (By similarity).
FT   METAL       244    244       Divalent metal cation (By similarity).
FT   METAL       267    267       Divalent metal cation (By similarity).
FT   BINDING     154    154       NAD (By similarity).
FT   BINDING     267    267       NAD (By similarity).
FT   BINDING     415    415       NAD (By similarity).
FT   SITE        267    267       Important for activity (By similarity).
SQ   SEQUENCE   562 AA;  62187 MW;  343B28DF025EB51D CRC64;
     MDDNKRPLYL PFAGPAILEA PLINKGSAFS EEERIFFNLE GLVPYAIETI EEQASRAYDQ
     FRSFNNDLDK HIYLRNIQDT NETLFYRLVQ NHISEMMPII YTPTVGLACE RFSKNYRRNR
     GLFISYPNKD RIDDILNNST RQKVKIIVVT DGERILGLGD QGIGGMGIPI GKLSLYTSCG
     GISPAYTLPI TLDVGTDNPQ LLEDPMYMGW RHPRIGGEEY AEFIEAFMQA VHVRWPDTLI
     QFEDFAQKNA MPILERYKDR YCCFNDDIQG TAAVTVGSLL AACKAAGTEL NKQRIAFLGA
     GSAGCGIAEA IVAQMVSEGI SDEQARSQVC MVDRWGLLLD NMPNLLPFQQ KLAQKCADIC
     NWNNFSDNIS LLDVVNNAKP TVLIGVSGAP GLFTEEIVRA MHSHCERPII FPLSNPTSRV
     EATPKDILHW TSGQALVATG SPFEPVVVDG ETYEIAQCNN SFIFPGIGLG VLASGARHVS
     DAMLMASSRA LAECSPLAIN GSGPLLPKLE DIHSVSKHIA FAVGKVAIEQ GLSLPASDEL
     LMQSIEDNFW KPEYRRYKRT SF
//