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A0KS65

- DAPF_SHESA

UniProt

A0KS65 - DAPF_SHESA

Protein

Diaminopimelate epimerase

Gene

dapF

Organism
Shewanella sp. (strain ANA-3)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 1 (12 Dec 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.UniRule annotation

    Catalytic activityi

    LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei12 – 121SubstrateUniRule annotation
    Binding sitei45 – 451SubstrateUniRule annotation
    Binding sitei65 – 651SubstrateUniRule annotation
    Active sitei74 – 741Proton donor/acceptorUniRule annotation
    Binding sitei158 – 1581SubstrateUniRule annotation
    Sitei160 – 1601Important for catalytic activityUniRule annotation
    Binding sitei191 – 1911SubstrateUniRule annotation
    Sitei209 – 2091Important for catalytic activityUniRule annotation
    Active sitei218 – 2181Proton donor/acceptorUniRule annotation

    GO - Molecular functioni

    1. diaminopimelate epimerase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Amino-acid biosynthesis, Lysine biosynthesis

    Enzyme and pathway databases

    BioCyciSSP94122:GJ9K-412-MONOMER.
    UniPathwayiUPA00034; UER00025.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diaminopimelate epimeraseUniRule annotation (EC:5.1.1.7UniRule annotation)
    Short name:
    DAP epimeraseUniRule annotation
    Gene namesi
    Name:dapFUniRule annotation
    Ordered Locus Names:Shewana3_0391
    OrganismiShewanella sp. (strain ANA-3)
    Taxonomic identifieri94122 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
    ProteomesiUP000002589: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 275275Diaminopimelate epimerasePRO_1000011966Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi74 ↔ 218

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Protein-protein interaction databases

    STRINGi94122.Shewana3_0391.

    Structurei

    3D structure databases

    ProteinModelPortaliA0KS65.
    SMRiA0KS65. Positions 2-275.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni9 – 102Substrate bindingUniRule annotation
    Regioni74 – 763Substrate bindingUniRule annotation
    Regioni209 – 2102Substrate bindingUniRule annotation
    Regioni219 – 2202Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the diaminopimelate epimerase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0253.
    HOGENOMiHOG000220466.
    KOiK01778.
    OMAiLIVEPPY.
    OrthoDBiEOG6ND0M5.

    Family and domain databases

    HAMAPiMF_00197. DAP_epimerase.
    InterProiIPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view]
    PANTHERiPTHR31689. PTHR31689. 1 hit.
    PfamiPF01678. DAP_epimerase. 2 hits.
    [Graphical view]
    TIGRFAMsiTIGR00652. DapF. 1 hit.
    PROSITEiPS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A0KS65-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIQFTKMHGL GNDFMVVDGV TQNVFFSPEQ IRRLADRNFG IGFDQLLLVE    50
    PPYDPDLDFH YRIFNADGGE VENCGNGARC FARFVRNKGL TNKNKIRVST 100
    SAGKMTLRLE RDGTVTVNMG VPVLDPSQIP FKAKKAEKTY LLQTPQQTFL 150
    CGAASMGNPH CVLDVEDVAN ANVAEIGALL TKHERFPRGV NVGFMQVVNS 200
    GHIKLRVYER GAAETLACGT GACAAVVVGQ IQGKLDQQVR VDLPGGTLTI 250
    NWEGEGKPLW MTGPAQHVYD GQIQL 275
    Length:275
    Mass (Da):30,107
    Last modified:December 12, 2006 - v1
    Checksum:i01E1771D9D77FCB5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000469 Genomic DNA. Translation: ABK46634.1.
    RefSeqiWP_011715615.1. NC_008577.1.
    YP_868040.1. NC_008577.1.

    Genome annotation databases

    EnsemblBacteriaiABK46634; ABK46634; Shewana3_0391.
    GeneIDi4478973.
    KEGGishn:Shewana3_0391.
    PATRICi23568358. VBISheSp134792_0639.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000469 Genomic DNA. Translation: ABK46634.1 .
    RefSeqi WP_011715615.1. NC_008577.1.
    YP_868040.1. NC_008577.1.

    3D structure databases

    ProteinModelPortali A0KS65.
    SMRi A0KS65. Positions 2-275.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 94122.Shewana3_0391.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABK46634 ; ABK46634 ; Shewana3_0391 .
    GeneIDi 4478973.
    KEGGi shn:Shewana3_0391.
    PATRICi 23568358. VBISheSp134792_0639.

    Phylogenomic databases

    eggNOGi COG0253.
    HOGENOMi HOG000220466.
    KOi K01778.
    OMAi LIVEPPY.
    OrthoDBi EOG6ND0M5.

    Enzyme and pathway databases

    UniPathwayi UPA00034 ; UER00025 .
    BioCyci SSP94122:GJ9K-412-MONOMER.

    Family and domain databases

    HAMAPi MF_00197. DAP_epimerase.
    InterProi IPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view ]
    PANTHERi PTHR31689. PTHR31689. 1 hit.
    Pfami PF01678. DAP_epimerase. 2 hits.
    [Graphical view ]
    TIGRFAMsi TIGR00652. DapF. 1 hit.
    PROSITEi PS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ANA-3.

    Entry informationi

    Entry nameiDAPF_SHESA
    AccessioniPrimary (citable) accession number: A0KS65
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: December 12, 2006
    Last modified: October 1, 2014
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3