Reviewed, UniProtKB/Swiss-Prot A0KRY0 (KATG1_SHESA)

Names and origin

Protein names

Recommended name:
    Catalase-peroxidase 1
      Short name=CP 1
    EC=1.11.1.6
    EC=1.11.1.7
Alternative name(s):
    Peroxidase/catalase 1

Gene names

Name:	katG1
Ordered Locus Names:	Shewana3_0306

Organism

Shewanella sp. (strain ANA-3) [Complete proteome] [HAMAP]

Taxonomic identifier

94122 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	728 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity.
Catalytic activity	2 H2O2 = O2 + 2 H2O. HAMAP MF_01961 Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity.
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity.
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Domain	Signal
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: HAMAP heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 728	706	Catalase-peroxidase 1 HAMAP MF_01961		PRO_0000354923
Sites
	Active site	98	1	Proton acceptor By similarity
	Metal binding	266	1	Iron (heme axial ligand) By similarity
	Site	94	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	97 ↔ 225		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-251) By similarity
	Cross-link	225 ↔ 251		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-97) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0KRY0-1 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 285760B6474BB5B4	FASTA	728	81,113
	10 20 30 40 50 60 MDKTQSSQGK CPVMHGANSA VASNNMDWWP KALNLDILHQ HDKKTDPMDP KFNYRDAFNS 70 80 90 100 110 120 LDLAAVKRDL NALMTDSQDW WPADWGHYGG LMIRMAWHSA GTYRVADGRG GAGTGNQRFA 130 140 150 160 170 180 PLNSWPDNAN LDKARRLLWP IKKKYGNKLS WADLMILAGN VAYESMGLKT YGFAGGREDI 190 200 210 220 230 240 WHPEKDIYWG SEKQWLAPTE NPNSRYSGER DLENPLAAVM MGLIYVNPEG VDGKPDPLRT 250 260 270 280 290 300 AQDVRVTFAR MAMNDEETVA LTAGGHTVGK CHGNGKAQDL GPEPEGEELE AQGLGWLNKK 310 320 330 340 350 360 GPGTGANAVT SGLEGAWTTH PTQWDNGYFH LLLNYDWELK KSPAGASQWE PINIKEEDKV 370 380 390 400 410 420 VSVGDPNRKF NPIMTDADMA MKMDPEYRKI SEKFYQDPAY FSEVFARAWF KLTHRDLGPK 430 440 450 460 470 480 SRYLGPEVPN EDLLWQDPIP SVDYRLDASE IVELKAKLLA SGLSVSDLVA TAWDSARTFR 490 500 510 520 530 540 GSDFRGGANG ARIRLAPQKD WQANEPERLQ KVLKVLIELQ ASLSKKVSIA DLIVLGGAAA 550 560 570 580 590 600 IEKAAHAAGV KITVPFIPGR GDATQEMTDV ESFAVLEPLH DAYRNWQKKD YVVQPEEMML 610 620 630 640 650 660 DRTQLMGLTA HEMTVLIGGM RVLGTNYGGT RHGVFTDKVG VLTNDFFVNL TDMAYNWKPA 670 680 690 700 710 720 GSNLYEIVER KTGAVKWTAT RVDLVFGSNS ILRSYAEVYA QDDAKEKFVH DFVAAWTKVM NADRFDLA

References

[1]

"Complete sequence of chromosome 1 of Shewanella sp. ANA-3." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Newman D., Salticov C., Konstantinidis K., Klappenback J., Tiedje J., Richardson P. Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000469 Genomic DNA. Translation: ABK46549.1.
RefSeq	YP_867955.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4479465.
GenomeReviews	Gene locus Shewana3_0306 in contig CP000469_GR.
KEGG	shn:Shewana3_0306.
NMPDR	fig|94122.5.peg.480.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A0KRY0. WPNALNL.
Family and domain databases
HAMAP	MF_01961. [Tree]
InterPro	IPR000763. Catalase_proxase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
TIGRFAMs	TIGR00198. cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. False negative. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG1_SHESA

Accession

Primary (citable) accession number: A0KRY0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	December 12, 2006
Last modified:	May 5, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)