A0KRF2 (RLMF_SHESA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase F
EC=2.1.1.181

Alternative name(s):
23S rRNA mA1618 methyltransferase
rRNA adenine N-6-methyltransferase

Gene names

Name:	rlmF
Ordered Locus Names:	Shewana3_0127

Organism

Shewanella sp. (strain ANA-3) [Complete proteome] [HAMAP]

Taxonomic identifier

94122 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the adenine in position 1618 of 23S rRNA By similarity. HAMAP MF_01848
Catalytic activity	S-adenosyl-L-methionine + adenine(1618) in 23S rRNA = S-adenosyl-L-homocysteine + rRNA containing N(6)-methyladenine(1618) in 23S rRNA. HAMAP MF_01848
Subcellular location	Cytoplasm Potential HAMAP MF_01848.
Sequence similarities	Belongs to the methyltransferase superfamily. METTL16/rlmF family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	rRNA (adenine-N6-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 364	364	Ribosomal RNA large subunit methyltransferase F HAMAP MF_01848		PRO_0000349962

Sequences

	Sequence		Length	Mass (Da)
	A0KRF2 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: C3A65819D10AFB97	FASTA	364	40,255
	10 20 30 40 50 60 MPKPAIKTAA KLAMSSAGKR GKPSTPKSLA KPQTTKPKTA SKLKAKHGEQ KRLHPRNLHI 70 80 90 100 110 120 NGYDFPALMA SFPKLKAFVR PTPYGALSID FADPFAVKNL NAALLKHHYG LAFWDIPKGA 130 140 150 160 170 180 LCPPIPGRVD YLHYLADLLF EGAKVKRAAA IHALDIGTGA NGVYAILGHQ VYDWQFVASD 190 200 210 220 230 240 INPQSLINVQ RIIDNNPSLQ GHLSLRRQQD DKAVFKGIIQ ASDRFELTLC NPPFHGSLKE 250 260 270 280 290 300 ASEGSLRKVR NLQLNRGEQP KATSATLNFG GQAAELWCQG GEKQFLATMI RESQAFAEQC 310 320 330 340 350 360 LWFTSLVSKQ ENLKPCYQAL EKLGVDTVKT IEMQQGNKVT RVLAWSFHSQ AKRLQWRNQV ISGA

References

[1]

"Complete sequence of chromosome 1 of Shewanella sp. ANA-3." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Newman D., Salticov C., Konstantinidis K., Klappenback J., Tiedje J., Richardson P. Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ANA-3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000469 Genomic DNA. Translation: ABK46371.1.
RefSeq	YP_867777.1. NC_008577.1.
3D structure databases
ProteinModelPortal	A0KRF2.
ModBase	Search...
Protein-protein interaction databases
STRING	A0KRF2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4477276.
GenomeReviews	Gene locus Shewana3_0127 in contig CP000469_GR.
KEGG	shn:Shewana3_0127.
NMPDR	fig|94122.5.peg.310.
PATRIC	23567770. VBISheSp134792_0362.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3129.
HOGENOM	HBG747880.
OMA	DFANPEA.
PhylomeDB	A0KRF2.
ProtClustDB	PRK11727.
Enzyme and pathway databases
BioCyc	SSP94122:SHEWANA3_0127-MONOMER.
Family and domain databases
HAMAP	MF_01848. 23SrRNA_methyltr_F. [Tree]
InterPro	IPR016909. rRNA_lsu_methyltransferase_F. IPR010286. SAM-Mtase_prd. [Graphical view]
KO	K06970.
PANTHER	PTHR13393. DUF890. 1 hit.
Pfam	PF05971. Methyltransf_10. 1 hit. [Graphical view]
PIRSF	PIRSF029038. Mtase_YbiN_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMF_SHESA

Accession

Primary (citable) accession number: A0KRF2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	December 12, 2006
Last modified:	December 14, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program