Reviewed, UniProtKB/Swiss-Prot A0KQV6 (TDH_AERHH)

Names and origin

Protein names

Recommended name:
    L-threonine 3-dehydrogenase
    EC=1.1.1.103

Gene names

Name:	tdh
Ordered Locus Names:	AHA_4235

Organism

Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) [Complete proteome] [HAMAP]

Taxonomic identifier

380703 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	342 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-threonine + NAD+ = L-2-amino-3-oxobutanoate + NADH. HAMAP MF_00627
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Amino-acid degradation; L-threonine degradation via oxydo-reductase pathway; glycine from L-threonine: step 1/2. HAMAP MF_00627
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding NAD Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW threonine catabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-threonine 3-dehydrogenase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 342	342	L-threonine 3-dehydrogenase HAMAP MF_00627		PRO_1000051612
Sites
	Metal binding	38	1	Zinc 1; catalytic By similarity
	Metal binding	63	1	Zinc 1; catalytic By similarity
	Metal binding	93	1	Zinc 2 By similarity
	Metal binding	96	1	Zinc 2 By similarity
	Metal binding	99	1	Zinc 2 By similarity
	Metal binding	107	1	Zinc 2 By similarity
	Metal binding	148	1	Zinc 1; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0KQV6-1 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: F4EB4BD579A402B8	FASTA	342	37,375
	10 20 30 40 50 60 MKALSKLHKE VGIWMTDVPA PELGHNDLLI KIRKTAICGT DIHIYNWDEW SQKTIPVPMV 70 80 90 100 110 120 VGHEYVGEVV AIGQEVRGFA VGDRVSGEGH ITCGHCRNCR AGRTHLCRNT IGVGVNRPGA 130 140 150 160 170 180 FAEYLVIPAF NAFKLPDNVP DELAAIFDPF GNAVHTALSF DLVGEDVLIT GAGPIGIMAA 190 200 210 220 230 240 AVCRHVGARH VVITDVNEYR LELARKMGAT RAVNVTKEQL SGVMSELGMT EGFDVGLEMS 250 260 270 280 290 300 GVPSAFQDMI DKMNHGGKIA MLGIPPATMA IDWGKVIFKG LFIKGIYGRE MFETWYKMAS 310 320 330 340 LIQSGLDLSP IITHRFHIDD FQQGFDAMRS GQSGKVILSW DK

References

[1]

"Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades." Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F. J. Bacteriol. 188:8272-8282(2006) [PubMed: 16980456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000462 Genomic DNA. Translation: ABK36533.1.
RefSeq	YP_858657.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A0KQV6.
Genome annotation databases
GeneID	4488004.
GenomeReviews	Gene locus AHA_4235 in contig CP000462_GR.
KEGG	aha:AHA_4235.
TIGR	AHA_4235.
Phylogenomic databases
OMA	IWMVDAP.
Family and domain databases
HAMAP	MF_00627. [Tree]
InterPro	IPR013154. ADH_GroES-like. IPR002085. ADH_SF_Zn. IPR013149. ADH_Zn-bd. IPR002328. ADH_Zn_CS. IPR004627. L-Threonine_3-DHase. [Graphical view]
PANTHER	PTHR11695. ADH_Sf_Zn. 1 hit.
Pfam	PF08240. ADH_N. 1 hit. PF00107. ADH_zinc_N. 1 hit. [Graphical view]
ProDom	PD040557. GroES_related. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00692. tdh. 1 hit.
PROSITE	PS00059. ADH_ZINC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TDH_AERHH

Accession

Primary (citable) accession number: A0KQV6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	December 12, 2006
Last modified:	November 3, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)