Reviewed, UniProtKB/Swiss-Prot A0KQS4 (ILVD_AERHH)

Names and origin

Protein names

Recommended name:
    Dihydroxy-acid dehydratase
      Short name=DAD
    EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	AHA_4202

Organism

Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) [Complete proteome] [HAMAP]

Taxonomic identifier

380703 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	613 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the ilvD/edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 613	613	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_0000321589
Sites
	Metal binding	122	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	195	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	A0KQS4-1 [UniParc]. Last modified February 26, 2008. Version 2. Checksum: A03F3C4996639856	FASTA	613	65,036
	10 20 30 40 50 60 MPKLRSATTT HGRNMAGARA LWRATGMTDQ DFGKPIIAVV NSFTQFVPGH VHLKDLGQLV 70 80 90 100 110 120 AREIEAAGGV AKEFNTIAVD DGIAMGHGGM LYSLPSRELI ADSVEYMVNA HCADAMVCIS 130 140 150 160 170 180 NCDKITPGML MAALRINIPV IFVSGGPMEA GKTKLSDQII KLDLVDAMIQ GADPKVSDAQ 190 200 210 220 230 240 SEQVERSACP TCGSCSGMFT ANSMNCLTEA LGLSQPGNGS LLATHSDREQ LFKLAGQRIV 250 260 270 280 290 300 TLAKRWYEQD DASALPRNIA TKAAFENAMA LDIAMGGSTN TVLHLLAAAQ EAGVDFTMAD 310 320 330 340 350 360 IDRMSRKVPQ LCKVAPSTQK YHMEDVHRAG GVVAILGQLE KAGLVHGDTR NVLGTSLVEL 370 380 390 400 410 420 LAEYDVSRQP SQEVVDFYRA GPAGIRTTKA FSQDCRWPEL DLDRAEGCIR SLDNAYSLEG 430 440 450 460 470 480 GLAVLAGNLA LNGAIVKTAG VDEENLTFRG PARVFESQDT AVAGILDGTV KAGEVVVIRY 490 500 510 520 530 540 EGPKGGPGMQ EMLYPTTYLK SMGLGKACAL ITDGRFSGGT SGLSIGHVSP EAASGGTIGL 550 560 570 580 590 600 VEDGDIINID IPARSMVLEV ADSVLAARRV AVEARGWKPL DRQRQVSFAL RAYAMFATSA 610 DKGAVRDRSM LGE

References

[1]

"Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades." Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F. J. Bacteriol. 188:8272-8282(2006) [PubMed: 16980456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000462 Genomic DNA. Translation: ABK36292.1. Different initiation.
RefSeq	YP_858625.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A0KQS4.
Genome annotation databases
GeneID	4489856.
GenomeReviews	Gene locus AHA_4202 in contig CP000462_GR.
KEGG	aha:AHA_4202.
TIGR	AHA_4202.
Family and domain databases
HAMAP	MF_00012. [Tree]
InterPro	IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
ProDom	PD002691. ILVD_EDD_family. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00110. ilvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_AERHH

Accession

Primary (citable) accession number: A0KQS4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	February 26, 2008
Last modified:	September 22, 2009
This is version 20 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)