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A0KQH4 (BGAL_AERHH) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase
Short name=Beta-gal
EC=3.2.1.23
Alternative name(s):
Lactase
Gene names
Name:lacZ
Ordered Locus Names:AHA_4101
OrganismAeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) [Complete proteome] [HAMAP]
Taxonomic identifier380703 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length1025 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. HAMAP MF_01687

Cofactor

Binds 2 magnesium ions per monomer By similarity. HAMAP MF_01687

Binds 1 sodium ion per monomer By similarity. HAMAP MF_01687

Subunit structure

Homotetramer By similarity. HAMAP MF_01687

Sequence similarities

Belongs to the glycosyl hydrolase 2 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10251025Beta-galactosidase HAMAP MF_01687
PRO_0000366979

Regions

Region538 – 5414Substrate binding By similarity

Sites

Active site4621Proton donor By similarity
Active site5381Nucleophile By similarity
Metal binding2041Sodium By similarity
Metal binding4171Magnesium 1 By similarity
Metal binding4191Magnesium 1 By similarity
Metal binding4621Magnesium 1 By similarity
Metal binding5981Magnesium 2 By similarity
Metal binding6021Sodium; via carbonyl oxygen By similarity
Metal binding6051Sodium By similarity
Binding site1051Substrate By similarity
Binding site2041Substrate By similarity
Binding site4621Substrate By similarity
Binding site6051Substrate By similarity
Binding site10031Substrate By similarity
Site3581Transition state stabilizer By similarity
Site3921Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
A0KQH4 [UniParc].

Last modified December 12, 2006. Version 1.
Checksum: 9358E7C1D095FAD1

FASTA1,025115,234
        10         20         30         40         50         60 
MLREIVARQD WQTQAITAVN RLPAHTPLFS WRSEAAARDD LASPSRTLLD GEWRFSFFEA 

        70         80         90        100        110        120 
PELVPEHWLV EDLPDACAIK VPGNWQLDAA YPGLRLATDV PIYTNIKYPF PCDPPRVPAE 

       130        140        150        160        170        180 
NPTGCYSREF SVPADWLASG QTRIIFDGVD SAFHLFCNGR WVGYSQDSRL PAEFDLTSFL 

       190        200        210        220        230        240 
CGGDNRLAVL VLRWSDGSYL EDQDMWRMSG IFRSVSLLHK PVRHLMDIRV TPELDACYRD 

       250        260        270        280        290        300 
GRLKIALQAA NGAGLSVAAC LYDGGERVAT LRQPIGTQAI DEKGAYDDRA ECWLEVAAPR 

       310        320        330        340        350        360 
KWSAETPHLY RLTLTLLDEQ GEPIESEAYD VGFRAVEIRG GLLRVNGQPL LIRGANRHEH 

       370        380        390        400        410        420 
DVASGHVVTP AAIEQDLLLM KRHNFNSVRC SHYPNHPELY RLCDRLGLYV VDEANLETHG 

       430        440        450        460        470        480 
MTPMGRLARD PAWSNAFLER VTRMVARDFN HPSIIIWSLG NESGYGPAHD AMYGWVKRAD 

       490        500        510        520        530        540 
PSRPVQYEGG GADTPATDII CPMYARTHQD QPFPAVPKWA LAKWIGLPGE TRPLILCEYA 

       550        560        570        580        590        600 
HAMGNSLGGY AHYWQAFRDH PRLQGGFVWD WVDQGLDKLT DDGRHFWAYG GDFGDTPNDR 

       610        620        630        640        650        660 
QFCCNGLVFP DRTPHPALFE ARRAQQPFGL TLLERQPLTV EIRSEYLFRE TDNERLQWRL 

       670        680        690        700        710        720 
CEDGVVVSQG ECPLTLAPQG SMMLTLLERL PAFAPGALAW LDLAIVQPAA TPWSAVGHEV 

       730        740        750        760        770        780 
ARQQCMLPAP LSLPVARTPA TFIELADGWQ IRAAASEWRL DKASGRVQSW CKLGREQLKE 

       790        800        810        820        830        840 
AIADHFYRAP LDNDIGTSEA DHADPNAWIA RWQEAGLNEL QHRCLDMVVS PDQGVVTVHH 

       850        860        870        880        890        900 
GYFVGDALKL LTRWRHEFDQ DGAMRLAIEV QVAAEMPSLP RIGARLWLTD EVLATGEEVS 

       910        920        930        940        950        960 
WLGRGPHENY PDRLLAADLG RWQSPLDVLH TAYVFPTDNG LRCDTRQLQL GSIEVEGLFH 

       970        980        990       1000       1010       1020 
FSLSRFSQQQ LAQARHQTDL VAEGGLHLCL DGFHMGIGGD DSWSQSVRPE YWLQPGGYYW 


NCVLR

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References

[1]"Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades."
Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F.
J. Bacteriol. 188:8272-8282(2006) [PubMed: 16980456] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 7966 / NCIB 9240.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000462 Genomic DNA. Translation: ABK36888.1.
RefSeqYP_858525.1. NC_008570.1.

3D structure databases

ProteinModelPortalA0KQH4.
ModBaseSearch...

Protein-protein interaction databases

STRINGA0KQH4.

Protein family/group databases

CAZyGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4490243.
GenomeReviewsGene locus AHA_4101 in contig CP000462_GR.
KEGGaha:AHA_4101.
PATRIC20785878. VBIAerHyd135212_4057.
TIGRAHA_4101.

Phylogenomic databases

eggNOGCOG3250.
HOGENOMHBG670643.
OMAHHINELE.
PhylomeDBA0KQH4.
ProtClustDBPRK09525.

Enzyme and pathway databases

BioCycAHYD196024:AHA_4101-MONOMER.

Family and domain databases

HAMAPMF_01687. Beta_gal.
[Tree]
InterProIPR004199. B-gal_small/dom_5.
IPR008979. Galactose-bd-like.
IPR011013. Glyco_hydro-type_carb-bd.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_subgr_catalytic.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
Gene3DG3DSA:2.60.40.320. Glyco_hydro_2/20_Ig-like. 2 hits.
G3DSA:2.70.98.10. Glyco_hydro_42_D5. 1 hit.
G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
KOK01190.
PfamPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSPR00132. GLHYDRLASE2.
SMARTSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMSSF49785. Gal_bind_like. 1 hit.
SSF74650. Gal_mut_like. 1 hit.
SSF49303. Glyco_hydro_2Ig. 2 hits.
SSF51445. Glyco_hydro_cat. 1 hit.
PROSITEPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL_AERHH
AccessionPrimary (citable) accession number: A0KQH4
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: December 12, 2006
Last modified: January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

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