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A0KQH4

- BGAL_AERHH

UniProt

A0KQH4 - BGAL_AERHH

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Protein
Beta-galactosidase
Gene
lacZ, AHA_4101
Organism
Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Binds 2 magnesium ions per monomer By similarity.UniRule annotation
Binds 1 sodium ion per monomer By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei105 – 1051Substrate By similarity
Metal bindingi204 – 2041Sodium By similarity
Binding sitei204 – 2041Substrate By similarity
Sitei358 – 3581Transition state stabilizer By similarity
Sitei392 – 3921Transition state stabilizer By similarity
Metal bindingi417 – 4171Magnesium 1 By similarity
Metal bindingi419 – 4191Magnesium 1 By similarity
Active sitei462 – 4621Proton donor By similarity
Metal bindingi462 – 4621Magnesium 1 By similarity
Binding sitei462 – 4621Substrate By similarity
Active sitei538 – 5381Nucleophile By similarity
Metal bindingi598 – 5981Magnesium 2 By similarity
Metal bindingi602 – 6021Sodium; via carbonyl oxygen By similarity
Metal bindingi605 – 6051Sodium By similarity
Binding sitei605 – 6051Substrate By similarity
Binding sitei1003 – 10031Substrate By similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Enzyme and pathway databases

BioCyciAHYD380703:GH2M-4101-MONOMER.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Short name:
Beta-gal
Alternative name(s):
Lactase
Gene namesi
Name:lacZ
Ordered Locus Names:AHA_4101
OrganismiAeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240)
Taxonomic identifieri380703 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeAeromonas
ProteomesiUP000000756: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10251025Beta-galactosidaseUniRule annotation
PRO_0000366979Add
BLAST

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi380703.AHA_4101.

Structurei

3D structure databases

ProteinModelPortaliA0KQH4.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni538 – 5414Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3250.
HOGENOMiHOG000252443.
KOiK01190.
OMAiNPPFVPK.
OrthoDBiEOG6XWV0T.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0KQH4-1 [UniParc]FASTAAdd to Basket

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MLREIVARQD WQTQAITAVN RLPAHTPLFS WRSEAAARDD LASPSRTLLD     50
GEWRFSFFEA PELVPEHWLV EDLPDACAIK VPGNWQLDAA YPGLRLATDV 100
PIYTNIKYPF PCDPPRVPAE NPTGCYSREF SVPADWLASG QTRIIFDGVD 150
SAFHLFCNGR WVGYSQDSRL PAEFDLTSFL CGGDNRLAVL VLRWSDGSYL 200
EDQDMWRMSG IFRSVSLLHK PVRHLMDIRV TPELDACYRD GRLKIALQAA 250
NGAGLSVAAC LYDGGERVAT LRQPIGTQAI DEKGAYDDRA ECWLEVAAPR 300
KWSAETPHLY RLTLTLLDEQ GEPIESEAYD VGFRAVEIRG GLLRVNGQPL 350
LIRGANRHEH DVASGHVVTP AAIEQDLLLM KRHNFNSVRC SHYPNHPELY 400
RLCDRLGLYV VDEANLETHG MTPMGRLARD PAWSNAFLER VTRMVARDFN 450
HPSIIIWSLG NESGYGPAHD AMYGWVKRAD PSRPVQYEGG GADTPATDII 500
CPMYARTHQD QPFPAVPKWA LAKWIGLPGE TRPLILCEYA HAMGNSLGGY 550
AHYWQAFRDH PRLQGGFVWD WVDQGLDKLT DDGRHFWAYG GDFGDTPNDR 600
QFCCNGLVFP DRTPHPALFE ARRAQQPFGL TLLERQPLTV EIRSEYLFRE 650
TDNERLQWRL CEDGVVVSQG ECPLTLAPQG SMMLTLLERL PAFAPGALAW 700
LDLAIVQPAA TPWSAVGHEV ARQQCMLPAP LSLPVARTPA TFIELADGWQ 750
IRAAASEWRL DKASGRVQSW CKLGREQLKE AIADHFYRAP LDNDIGTSEA 800
DHADPNAWIA RWQEAGLNEL QHRCLDMVVS PDQGVVTVHH GYFVGDALKL 850
LTRWRHEFDQ DGAMRLAIEV QVAAEMPSLP RIGARLWLTD EVLATGEEVS 900
WLGRGPHENY PDRLLAADLG RWQSPLDVLH TAYVFPTDNG LRCDTRQLQL 950
GSIEVEGLFH FSLSRFSQQQ LAQARHQTDL VAEGGLHLCL DGFHMGIGGD 1000
DSWSQSVRPE YWLQPGGYYW NCVLR 1025
Length:1,025
Mass (Da):115,234
Last modified:December 12, 2006 - v1
Checksum:i9358E7C1D095FAD1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000462 Genomic DNA. Translation: ABK36888.1.
RefSeqiWP_011707763.1. NC_008570.1.
YP_858525.1. NC_008570.1.

Genome annotation databases

EnsemblBacteriaiABK36888; ABK36888; AHA_4101.
GeneIDi4490243.
KEGGiaha:AHA_4101.
PATRICi20785878. VBIAerHyd135212_4057.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000462 Genomic DNA. Translation: ABK36888.1 .
RefSeqi WP_011707763.1. NC_008570.1.
YP_858525.1. NC_008570.1.

3D structure databases

ProteinModelPortali A0KQH4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 380703.AHA_4101.

Protein family/group databases

CAZyi GH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABK36888 ; ABK36888 ; AHA_4101 .
GeneIDi 4490243.
KEGGi aha:AHA_4101.
PATRICi 20785878. VBIAerHyd135212_4057.

Phylogenomic databases

eggNOGi COG3250.
HOGENOMi HOG000252443.
KOi K01190.
OMAi NPPFVPK.
OrthoDBi EOG6XWV0T.

Enzyme and pathway databases

BioCyci AHYD380703:GH2M-4101-MONOMER.

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_01687. Beta_gal.
InterProi IPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
PRINTSi PR00132. GLHYDRLASE2.
SMARTi SM01038. Bgal_small_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 7966 / NCIB 9240.

Entry informationi

Entry nameiBGAL_AERHH
AccessioniPrimary (citable) accession number: A0KQH4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: December 12, 2006
Last modified: September 3, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi