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A0KQH4

- BGAL_AERHH

UniProt

A0KQH4 - BGAL_AERHH

Protein

Beta-galactosidase

Gene

lacZ

Organism
Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 52 (01 Oct 2014)
      Sequence version 1 (12 Dec 2006)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

    Cofactori

    Binds 2 magnesium ions per monomer.UniRule annotation
    Binds 1 sodium ion per monomer.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei105 – 1051SubstrateUniRule annotation
    Metal bindingi204 – 2041SodiumUniRule annotation
    Binding sitei204 – 2041SubstrateUniRule annotation
    Sitei358 – 3581Transition state stabilizerUniRule annotation
    Sitei392 – 3921Transition state stabilizerUniRule annotation
    Metal bindingi417 – 4171Magnesium 1UniRule annotation
    Metal bindingi419 – 4191Magnesium 1UniRule annotation
    Active sitei462 – 4621Proton donorUniRule annotation
    Metal bindingi462 – 4621Magnesium 1UniRule annotation
    Binding sitei462 – 4621SubstrateUniRule annotation
    Active sitei538 – 5381NucleophileUniRule annotation
    Metal bindingi598 – 5981Magnesium 2UniRule annotation
    Metal bindingi602 – 6021Sodium; via carbonyl oxygenUniRule annotation
    Metal bindingi605 – 6051SodiumUniRule annotation
    Binding sitei605 – 6051SubstrateUniRule annotation
    Binding sitei1003 – 10031SubstrateUniRule annotation

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC
    2. carbohydrate binding Source: InterPro
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding, Sodium

    Enzyme and pathway databases

    BioCyciAHYD380703:GH2M-4101-MONOMER.

    Protein family/group databases

    CAZyiGH2. Glycoside Hydrolase Family 2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
    Short name:
    Beta-galUniRule annotation
    Alternative name(s):
    LactaseUniRule annotation
    Gene namesi
    Name:lacZUniRule annotation
    Ordered Locus Names:AHA_4101
    OrganismiAeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240)
    Taxonomic identifieri380703 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeAeromonas
    ProteomesiUP000000756: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. beta-galactosidase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10251025Beta-galactosidasePRO_0000366979Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi380703.AHA_4101.

    Structurei

    3D structure databases

    ProteinModelPortaliA0KQH4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni538 – 5414Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 2 family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG3250.
    HOGENOMiHOG000252443.
    KOiK01190.
    OMAiNPPFVPK.
    OrthoDBiEOG6XWV0T.

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPiMF_01687. Beta_gal.
    InterProiIPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view]
    PRINTSiPR00132. GLHYDRLASE2.
    SMARTiSM01038. Bgal_small_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A0KQH4-1 [UniParc]FASTAAdd to Basket

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    MLREIVARQD WQTQAITAVN RLPAHTPLFS WRSEAAARDD LASPSRTLLD     50
    GEWRFSFFEA PELVPEHWLV EDLPDACAIK VPGNWQLDAA YPGLRLATDV 100
    PIYTNIKYPF PCDPPRVPAE NPTGCYSREF SVPADWLASG QTRIIFDGVD 150
    SAFHLFCNGR WVGYSQDSRL PAEFDLTSFL CGGDNRLAVL VLRWSDGSYL 200
    EDQDMWRMSG IFRSVSLLHK PVRHLMDIRV TPELDACYRD GRLKIALQAA 250
    NGAGLSVAAC LYDGGERVAT LRQPIGTQAI DEKGAYDDRA ECWLEVAAPR 300
    KWSAETPHLY RLTLTLLDEQ GEPIESEAYD VGFRAVEIRG GLLRVNGQPL 350
    LIRGANRHEH DVASGHVVTP AAIEQDLLLM KRHNFNSVRC SHYPNHPELY 400
    RLCDRLGLYV VDEANLETHG MTPMGRLARD PAWSNAFLER VTRMVARDFN 450
    HPSIIIWSLG NESGYGPAHD AMYGWVKRAD PSRPVQYEGG GADTPATDII 500
    CPMYARTHQD QPFPAVPKWA LAKWIGLPGE TRPLILCEYA HAMGNSLGGY 550
    AHYWQAFRDH PRLQGGFVWD WVDQGLDKLT DDGRHFWAYG GDFGDTPNDR 600
    QFCCNGLVFP DRTPHPALFE ARRAQQPFGL TLLERQPLTV EIRSEYLFRE 650
    TDNERLQWRL CEDGVVVSQG ECPLTLAPQG SMMLTLLERL PAFAPGALAW 700
    LDLAIVQPAA TPWSAVGHEV ARQQCMLPAP LSLPVARTPA TFIELADGWQ 750
    IRAAASEWRL DKASGRVQSW CKLGREQLKE AIADHFYRAP LDNDIGTSEA 800
    DHADPNAWIA RWQEAGLNEL QHRCLDMVVS PDQGVVTVHH GYFVGDALKL 850
    LTRWRHEFDQ DGAMRLAIEV QVAAEMPSLP RIGARLWLTD EVLATGEEVS 900
    WLGRGPHENY PDRLLAADLG RWQSPLDVLH TAYVFPTDNG LRCDTRQLQL 950
    GSIEVEGLFH FSLSRFSQQQ LAQARHQTDL VAEGGLHLCL DGFHMGIGGD 1000
    DSWSQSVRPE YWLQPGGYYW NCVLR 1025
    Length:1,025
    Mass (Da):115,234
    Last modified:December 12, 2006 - v1
    Checksum:i9358E7C1D095FAD1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000462 Genomic DNA. Translation: ABK36888.1.
    RefSeqiWP_011707763.1. NC_008570.1.
    YP_858525.1. NC_008570.1.

    Genome annotation databases

    EnsemblBacteriaiABK36888; ABK36888; AHA_4101.
    GeneIDi4490243.
    KEGGiaha:AHA_4101.
    PATRICi20785878. VBIAerHyd135212_4057.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000462 Genomic DNA. Translation: ABK36888.1 .
    RefSeqi WP_011707763.1. NC_008570.1.
    YP_858525.1. NC_008570.1.

    3D structure databases

    ProteinModelPortali A0KQH4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 380703.AHA_4101.

    Protein family/group databases

    CAZyi GH2. Glycoside Hydrolase Family 2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABK36888 ; ABK36888 ; AHA_4101 .
    GeneIDi 4490243.
    KEGGi aha:AHA_4101.
    PATRICi 20785878. VBIAerHyd135212_4057.

    Phylogenomic databases

    eggNOGi COG3250.
    HOGENOMi HOG000252443.
    KOi K01190.
    OMAi NPPFVPK.
    OrthoDBi EOG6XWV0T.

    Enzyme and pathway databases

    BioCyci AHYD380703:GH2M-4101-MONOMER.

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPi MF_01687. Beta_gal.
    InterProi IPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00132. GLHYDRLASE2.
    SMARTi SM01038. Bgal_small_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 7966 / NCIB 9240.

    Entry informationi

    Entry nameiBGAL_AERHH
    AccessioniPrimary (citable) accession number: A0KQH4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: December 12, 2006
    Last modified: October 1, 2014
    This is version 52 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3