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Protein

Beta-galactosidase

Gene

lacZ

Organism
Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 2 magnesium ions per monomer.UniRule annotation
  • Na(+)UniRule annotationNote: Binds 1 sodium ion per monomer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei105 – 1051SubstrateUniRule annotation
Metal bindingi204 – 2041SodiumUniRule annotation
Binding sitei204 – 2041SubstrateUniRule annotation
Sitei358 – 3581Transition state stabilizerUniRule annotation
Sitei392 – 3921Transition state stabilizerUniRule annotation
Metal bindingi417 – 4171Magnesium 1UniRule annotation
Metal bindingi419 – 4191Magnesium 1UniRule annotation
Active sitei462 – 4621Proton donorUniRule annotation
Metal bindingi462 – 4621Magnesium 1UniRule annotation
Binding sitei462 – 4621SubstrateUniRule annotation
Active sitei538 – 5381NucleophileUniRule annotation
Metal bindingi598 – 5981Magnesium 2UniRule annotation
Metal bindingi602 – 6021Sodium; via carbonyl oxygenUniRule annotation
Metal bindingi605 – 6051SodiumUniRule annotation
Binding sitei605 – 6051SubstrateUniRule annotation
Binding sitei1003 – 10031SubstrateUniRule annotation

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Enzyme and pathway databases

BioCyciAHYD380703:GH2M-4101-MONOMER.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
Short name:
Beta-galUniRule annotation
Alternative name(s):
LactaseUniRule annotation
Gene namesi
Name:lacZUniRule annotation
Ordered Locus Names:AHA_4101
OrganismiAeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240)
Taxonomic identifieri380703 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeAeromonas
ProteomesiUP000000756 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10251025Beta-galactosidasePRO_0000366979Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi380703.AHA_4101.

Structurei

3D structure databases

ProteinModelPortaliA0KQH4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni538 – 5414Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG3250.
HOGENOMiHOG000252443.
KOiK01190.
OMAiAIIPSFW.
OrthoDBiEOG6XWV0T.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0KQH4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLREIVARQD WQTQAITAVN RLPAHTPLFS WRSEAAARDD LASPSRTLLD
60 70 80 90 100
GEWRFSFFEA PELVPEHWLV EDLPDACAIK VPGNWQLDAA YPGLRLATDV
110 120 130 140 150
PIYTNIKYPF PCDPPRVPAE NPTGCYSREF SVPADWLASG QTRIIFDGVD
160 170 180 190 200
SAFHLFCNGR WVGYSQDSRL PAEFDLTSFL CGGDNRLAVL VLRWSDGSYL
210 220 230 240 250
EDQDMWRMSG IFRSVSLLHK PVRHLMDIRV TPELDACYRD GRLKIALQAA
260 270 280 290 300
NGAGLSVAAC LYDGGERVAT LRQPIGTQAI DEKGAYDDRA ECWLEVAAPR
310 320 330 340 350
KWSAETPHLY RLTLTLLDEQ GEPIESEAYD VGFRAVEIRG GLLRVNGQPL
360 370 380 390 400
LIRGANRHEH DVASGHVVTP AAIEQDLLLM KRHNFNSVRC SHYPNHPELY
410 420 430 440 450
RLCDRLGLYV VDEANLETHG MTPMGRLARD PAWSNAFLER VTRMVARDFN
460 470 480 490 500
HPSIIIWSLG NESGYGPAHD AMYGWVKRAD PSRPVQYEGG GADTPATDII
510 520 530 540 550
CPMYARTHQD QPFPAVPKWA LAKWIGLPGE TRPLILCEYA HAMGNSLGGY
560 570 580 590 600
AHYWQAFRDH PRLQGGFVWD WVDQGLDKLT DDGRHFWAYG GDFGDTPNDR
610 620 630 640 650
QFCCNGLVFP DRTPHPALFE ARRAQQPFGL TLLERQPLTV EIRSEYLFRE
660 670 680 690 700
TDNERLQWRL CEDGVVVSQG ECPLTLAPQG SMMLTLLERL PAFAPGALAW
710 720 730 740 750
LDLAIVQPAA TPWSAVGHEV ARQQCMLPAP LSLPVARTPA TFIELADGWQ
760 770 780 790 800
IRAAASEWRL DKASGRVQSW CKLGREQLKE AIADHFYRAP LDNDIGTSEA
810 820 830 840 850
DHADPNAWIA RWQEAGLNEL QHRCLDMVVS PDQGVVTVHH GYFVGDALKL
860 870 880 890 900
LTRWRHEFDQ DGAMRLAIEV QVAAEMPSLP RIGARLWLTD EVLATGEEVS
910 920 930 940 950
WLGRGPHENY PDRLLAADLG RWQSPLDVLH TAYVFPTDNG LRCDTRQLQL
960 970 980 990 1000
GSIEVEGLFH FSLSRFSQQQ LAQARHQTDL VAEGGLHLCL DGFHMGIGGD
1010 1020
DSWSQSVRPE YWLQPGGYYW NCVLR
Length:1,025
Mass (Da):115,234
Last modified:December 12, 2006 - v1
Checksum:i9358E7C1D095FAD1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000462 Genomic DNA. Translation: ABK36888.1.
RefSeqiYP_858525.1. NC_008570.1.

Genome annotation databases

EnsemblBacteriaiABK36888; ABK36888; AHA_4101.
GeneIDi4490243.
KEGGiaha:AHA_4101.
PATRICi20785878. VBIAerHyd135212_4057.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000462 Genomic DNA. Translation: ABK36888.1.
RefSeqiYP_858525.1. NC_008570.1.

3D structure databases

ProteinModelPortaliA0KQH4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi380703.AHA_4101.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK36888; ABK36888; AHA_4101.
GeneIDi4490243.
KEGGiaha:AHA_4101.
PATRICi20785878. VBIAerHyd135212_4057.

Phylogenomic databases

eggNOGiCOG3250.
HOGENOMiHOG000252443.
KOiK01190.
OMAiAIIPSFW.
OrthoDBiEOG6XWV0T.

Enzyme and pathway databases

BioCyciAHYD380703:GH2M-4101-MONOMER.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 7966 / NCIB 9240.

Entry informationi

Entry nameiBGAL_AERHH
AccessioniPrimary (citable) accession number: A0KQH4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: December 12, 2006
Last modified: April 1, 2015
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.