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Protein

Bifunctional ligase/repressor BirA

Gene

birA

Organism
Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Acts both as a biotin--[acetyl-CoA-carboxylase] ligase and a biotin-operon repressor. In the presence of ATP, BirA activates biotin to form the BirA-biotinyl-5'-adenylate (BirA-bio-5'-AMP or holoBirA) complex. HoloBirA can either transfer the biotinyl moiety to the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase, or bind to the biotin operator site and inhibit transcription of the operon.UniRule annotation

Catalytic activityi

ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)].UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei112 – 1121BiotinUniRule annotation
Binding sitei183 – 1831BiotinUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi22 – 4120H-T-H motifUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. biotin-[acetyl-CoA-carboxylase] ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellular protein modification process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationImported, RepressorUniRule annotation

Keywords - Biological processi

Transcription, Transcription regulationUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, BiotinUniRule annotation, DNA-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciAHYD380703:GH2M-4040-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional ligase/repressor BirAUniRule annotation
Alternative name(s):
Biotin operon repressorUniRule annotation
Biotin--[acetyl-CoA-carboxylase] ligaseUniRule annotation
Biotin--protein ligaseUniRule annotation
Biotin-[acetyl-CoA carboxylase] synthetaseUniRule annotation
Gene namesi
Name:birAUniRule annotation
Ordered Locus Names:AHA_4040Imported
OrganismiAeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240)Imported
Taxonomic identifieri380703 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeAeromonas
ProteomesiUP000000756 Componenti: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi380703.AHA_4040.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini65 – 255191BPL/LPL catalyticUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni89 – 913Biotin bindingUniRule annotation
Regioni116 – 1183Biotin bindingUniRule annotation

Sequence similaritiesi

Belongs to the biotin--protein ligase family.UniRule annotation
Contains 1 BPL/LPL catalytic domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0340.
HOGENOMiHOG000041812.
KOiK03524.
OMAiIYSADIE.
OrthoDBiEOG6DNTBX.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_00978. Bifunct_BirA.
InterProiIPR030855. Bifunct_BirA.
IPR004408. Biotin_CoA_COase_ligase.
IPR003142. BPL_C.
IPR004143. BPL_LipA_LipB.
IPR013196. HTH_11.
IPR008988. Transcriptional_repressor_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR12835. PTHR12835. 1 hit.
PfamiPF02237. BPL_C. 1 hit.
PF03099. BPL_LplA_LipB. 1 hit.
PF08279. HTH_11. 1 hit.
[Graphical view]
SUPFAMiSSF50037. SSF50037. 1 hit.
TIGRFAMsiTIGR00121. birA_ligase. 1 hit.

Sequencei

Sequence statusi: Complete.

A0KQB4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLTPIRQTL ITLLSDGQFH SGEQLGEQLG ISRAAVSKHM AALKELGLDL
60 70 80 90 100
FSLTGKGYRL AVPMALYDEV QLQALAPMAP VHCFPVIDST NQYLLERVNQ
110 120 130 140 150
LSSGESCLAE CQTAGRGRRG KPWVSPFGCQ LILSMYWRLE QGMAAAMGLS
160 170 180 190 200
LAVGVAVVQA LESLGYPGVE LKWPNDLYYQ GRKLAGILVE MSGSAGASCH
210 220 230 240 250
LVIGVGLNLA MPSREGERID QAWSELRHIQ PELVDRNLLA GRVLDHLQQA
260 270 280 290 300
MLTFEQSGLA SFVADWNRLD HFAGRPVRLL MGEQEIRGVA RGIDDRGALR
310 320
LETAEGIKFY LGGEISLRRG D
Length:321
Mass (Da):35,085
Last modified:December 12, 2006 - v1
Checksum:i6DB906F23C0636C9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000462 Genomic DNA. Translation: ABK37252.1.
RefSeqiWP_011707707.1. NC_008570.1.
YP_858465.1. NC_008570.1.

Genome annotation databases

EnsemblBacteriaiABK37252; ABK37252; AHA_4040.
GeneIDi4488039.
KEGGiaha:AHA_4040.
PATRICi20785758. VBIAerHyd135212_3998.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000462 Genomic DNA. Translation: ABK37252.1.
RefSeqiWP_011707707.1. NC_008570.1.
YP_858465.1. NC_008570.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi380703.AHA_4040.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK37252; ABK37252; AHA_4040.
GeneIDi4488039.
KEGGiaha:AHA_4040.
PATRICi20785758. VBIAerHyd135212_3998.

Phylogenomic databases

eggNOGiCOG0340.
HOGENOMiHOG000041812.
KOiK03524.
OMAiIYSADIE.
OrthoDBiEOG6DNTBX.

Enzyme and pathway databases

BioCyciAHYD380703:GH2M-4040-MONOMER.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_00978. Bifunct_BirA.
InterProiIPR030855. Bifunct_BirA.
IPR004408. Biotin_CoA_COase_ligase.
IPR003142. BPL_C.
IPR004143. BPL_LipA_LipB.
IPR013196. HTH_11.
IPR008988. Transcriptional_repressor_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR12835. PTHR12835. 1 hit.
PfamiPF02237. BPL_C. 1 hit.
PF03099. BPL_LplA_LipB. 1 hit.
PF08279. HTH_11. 1 hit.
[Graphical view]
SUPFAMiSSF50037. SSF50037. 1 hit.
TIGRFAMsiTIGR00121. birA_ligase. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240Imported.

Entry informationi

Entry nameiA0KQB4_AERHH
AccessioniPrimary (citable) accession number: A0KQB4
Entry historyi
Integrated into UniProtKB/TrEMBL: December 12, 2006
Last sequence update: December 12, 2006
Last modified: April 29, 2015
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.