Reviewed, UniProtKB/Swiss-Prot A0KPX1 (MURC_AERHH)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramate--L-alanine ligase
    EC=6.3.2.8
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	AHA_3883

Organism

Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) [Complete proteome] [HAMAP]

Taxonomic identifier

380703 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	484 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 484	484	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_1000071092
Regions
	Nucleotide binding	126 – 132	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A0KPX1-1 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 48B588E3A253ECC0	FASTA	484	52,326
	10 20 30 40 50 60 MTKVELAKLR TVIPEMRRVR RIHFIGIGGA GMGGIAEVLA NEGYQVTGSD LARNAVTERL 70 80 90 100 110 120 ESLGAHIFVG HSAEHVNEAS VVVVSTAIKQ DNPELLAARE LRIPVVRRAE MLAELMRFRH 130 140 150 160 170 180 GIAIAGTHGK TTTTSLVASI YAEADRDPTF VIGGLLNSAG TNARLGSSRY LIAEADESDA 190 200 210 220 230 240 SFLHLQPMVS IVTNIEADHM DTYGGDFSKL KATFIDFLHN LPFYGLAVVC VDDPVIRGML 250 260 270 280 290 300 PEIARPTITY GLSDDADVQV LDFVQTSNRS RFRVRRKDAG ELEVTLNLPG IHNALNAAAA 310 320 330 340 350 360 IAVATEDGIG DDAIIRALAK FEGVGRRFQQ YGEFETGRGK AMLVDDYGHH PSEVKVTINA 370 380 390 400 410 420 ARAGWPEKRL VMVFQPHRYT RTRDLYEDFA DVLSKVDVLV MLEVYAAGEE PIPGADGRAL 430 440 450 460 470 480 CRSIRSRGSL EPIFVATPDD VPAVLAGLVG EGDLVLTQGA GNVGALARRL GEMKLSIESM KSGA

References

[1]

"Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades." Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F. J. Bacteriol. 188:8272-8282(2006) [PubMed: 16980456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000462 Genomic DNA. Translation: ABK37350.1.
RefSeq	YP_858322.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A0KPX1.
Genome annotation databases
GeneID	4490853.
GenomeReviews	Gene locus AHA_3883 in contig CP000462_GR.
KEGG	aha:AHA_3883.
TIGR	AHA_3883.
Phylogenomic databases
OMA	EWMVVEA.
Family and domain databases
HAMAP	MF_00046. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01082. murC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_AERHH

Accession

Primary (citable) accession number: A0KPX1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	December 12, 2006
Last modified:	November 3, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)