Reviewed, UniProtKB/Swiss-Prot A0KPF3 (AMPA_AERHH)

Names and origin

Protein names

Recommended name:
    Probable cytosol aminopeptidase
    EC=3.4.11.1
Alternative name(s):
    Leucine aminopeptidase
      Short name=LAP
    Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	AHA_3700

Organism

Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) [Complete proteome] [HAMAP]

Taxonomic identifier

380703 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	502 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity.
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181
Cofactor	Binds 2 manganese ions per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP metalloexopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 502	502	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_1000019875
Sites
	Active site	279	1	Potential
	Active site	353	1	Potential
	Metal binding	267	1	Manganese 2 By similarity
	Metal binding	272	1	Manganese 1 By similarity
	Metal binding	272	1	Manganese 2 By similarity
	Metal binding	290	1	Manganese 2 By similarity
	Metal binding	349	1	Manganese 1 By similarity
	Metal binding	351	1	Manganese 1 By similarity
	Metal binding	351	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0KPF3-1 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 7C0A7A2046443A09	FASTA	502	54,666
	10 20 30 40 50 60 MEFSVKSGSP EKQRSACVVV GVFEPRRLSP VAEQLDKISD GYISSLLRRG DLEGKPGQML 70 80 90 100 110 120 LLHQVPGVLS ERVLLVGCGK ERELDERQYK QIINKTITTL NETGSMEAVC FLTELHVKGR 130 140 150 160 170 180 DTYWKVRQAV ETTKAGLYSF DQFKTNKAEP RRPLRKLVFN VPTRRELTIG EKAIAHGLAV 190 200 210 220 230 240 AKGVRVCRDV ANMPPNVCNP AYLASQARRL ADAFDNITTK VIGEQEMAEL GMNSYLAVAR 250 260 270 280 290 300 GSDNEAMMAI IEYKGHPDAK PIVLVGKGLT FDSGGISIKP AEGMDEMKYD MGGAASVLGT 310 320 330 340 350 360 MHALAQLQLP INVIGVLAGC ENMPGGNAYR PGDILTSMSG QTIEVLNTDA EGRLVLCDAL 370 380 390 400 410 420 TYVDRFDPET VIDVATLTGA CIIALGHHTT GLLANHNPLA HELLNASEQA GDRAWRLPLF 430 440 450 460 470 480 DEYQEQLESP FADMANIGGR PAGTITAAAF LSRFTKKYNW AHLDIAGTAW KSGKEKGSTG 490 500 RPVPLLTQFL LNRSGVVIEE KE

References

[1]

"Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades." Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F. J. Bacteriol. 188:8272-8282(2006) [PubMed: 16980456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000462 Genomic DNA. Translation: ABK38760.1.
RefSeq	YP_858154.1.
3D structure databases
SMR	A0KPF3. Positions 1-500.
ModBase	Search...
Protein-protein interaction databases
STRING	A0KPF3.
Protein family/group databases
MEROPS	M17.003.
Genome annotation databases
GeneID	4488026.
GenomeReviews	Gene locus AHA_3700 in contig CP000462_GR.
KEGG	aha:AHA_3700.
TIGR	AHA_3700.
Phylogenomic databases
OMA	LGHHISG.
Family and domain databases
HAMAP	MF_00181. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR008283. Peptidase_M17_N. [Graphical view]
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_AERHH

Accession

Primary (citable) accession number: A0KPF3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	December 12, 2006
Last modified:	November 3, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)