Reviewed, UniProtKB/Swiss-Prot A0KP08 (PANC_AERHH)

Names and origin

Protein names

Recommended name:
    Pantothenate synthetase
      Short name=PS
    EC=6.3.2.1
Alternative name(s):
    Pantoate--beta-alanine ligase
    Pantoate-activating enzyme

Gene names

Name:	panC
Ordered Locus Names:	AHA_3539

Organism

Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) [Complete proteome] [HAMAP]

Taxonomic identifier

380703 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	285 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate By similarity.
Catalytic activity	ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate. HAMAP MF_00158
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantothenate from (R)-pantoate and beta-alanine: step 1/1. HAMAP MF_00158
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential.
Miscellaneous	The reaction proceeds by a bi uni uni bi ping pong mechanism By similarity.
Sequence similarities	Belongs to the pantothenate synthetase family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pantothenate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW pantoate-beta-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 285	285	Pantothenate synthetase HAMAP MF_00158		PRO_0000305386
Regions
	Nucleotide binding	30 – 37	8	ATP By similarity
	Nucleotide binding	149 – 152	4	ATP By similarity
	Nucleotide binding	186 – 189	4	ATP By similarity
Sites
	Active site	37	1	Proton donor By similarity
	Binding site	61	1	Beta-alanine By similarity
	Binding site	61	1	Pantoate By similarity
	Binding site	155	1	Pantoate By similarity
	Binding site	178	1	ATP; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0KP08-1 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 243CEBB6570ABD89	FASTA	285	31,100
	10 20 30 40 50 60 MLVVNNPAAL REQIGQWRRE GLTIAFVPTM GNLHQGHLTL VKEAHHHADK VVTSIFVNPM 70 80 90 100 110 120 QFDKAEDLAN YPRTLEQDCA ALETAGVDMV FTPTPEIMYP QGLASHTFVE VPGLSGLLEG 130 140 150 160 170 180 ALRPGHFRGV STVVTKLFNL VQPDVACFGQ KDYQQLALIR KMVADMAMPI QIVGVPTVRA 190 200 210 220 230 240 EDGLALSSRN GYLTTAERAL APELARTMNW IAEQIEAGDH HLPSLVAQAS QRLDKAGFRT 250 260 270 280 DAIDIVDAAT LEAATDQSEQ LVILMAAYLG KARLIDNRVV TLARS

References

[1]

"Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades." Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F. J. Bacteriol. 188:8272-8282(2006) [PubMed: 16980456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000462 Genomic DNA. Translation: ABK36931.1.
RefSeq	YP_858009.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A0KP08.
Genome annotation databases
GeneID	4488711.
GenomeReviews	Gene locus AHA_3539 in contig CP000462_GR.
KEGG	aha:AHA_3539.
TIGR	AHA_3539.
Phylogenomic databases
OMA	VADFNIP.
Family and domain databases
HAMAP	MF_00158. [Tree]
InterPro	IPR004821. Cyt_trans_rel. IPR003721. Pantoate_ligase. [Graphical view]
PANTHER	PTHR21299:SF1. Pantoate_ligase. 1 hit.
Pfam	PF02569. Pantoate_ligase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00125. cyt_tran_rel. 1 hit. TIGR00018. panC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANC_AERHH

Accession

Primary (citable) accession number: A0KP08

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	December 12, 2006
Last modified:	November 3, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)