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A0KNV5 (TRMB_AERHH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:AHA_3482
OrganismAeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) [Complete proteome] [HAMAP]
Taxonomic identifier380703 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeAeromonas

Protein attributes

Sequence length231 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 231231tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000288114

Regions

Region209 – 2124Substrate binding By similarity

Sites

Active site1361 By similarity
Binding site611S-adenosyl-L-methionine By similarity
Binding site861S-adenosyl-L-methionine By similarity
Binding site1131S-adenosyl-L-methionine By similarity
Binding site1361S-adenosyl-L-methionine By similarity
Binding site1401Substrate By similarity
Binding site1721Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
A0KNV5 [UniParc].

Last modified December 12, 2006. Version 1.
Checksum: B8275BAF50F9B497

FASTA23126,094
        10         20         30         40         50         60 
MFNEDGKFMR KIRSFVRREG RLTKGQEKAL EELWPVMGID FAPEPLDLVA LFGREAPVVL 

        70         80         90        100        110        120 
EIGFGMGASL VEMAKNAPEK NFIGIEVHSP GVGACLGTAQ EAGVTNLRVI CHDAVEVLEH 

       130        140        150        160        170        180 
MIPNGSLACL QLFFPDPWHK SRHHKRRIVQ PAFVQDIRQK LAIGGVFHMA TDWENYAEHM 

       190        200        210        220        230 
LEVMSAAEGY ENTSATGNWV PRPDWRPLTK FEQRGHRLGH GVWDLIFKRV N 

« Hide

References

[1]"Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades."
Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F.
J. Bacteriol. 188:8272-8282(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 7966 / NCIB 9240.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000462 Genomic DNA. Translation: ABK37533.1.
RefSeqYP_857956.1. NC_008570.1.

3D structure databases

ProteinModelPortalA0KNV5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING380703.AHA_3482.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABK37533; ABK37533; AHA_3482.
GeneID4487817.
KEGGaha:AHA_3482.
PATRIC20784606. VBIAerHyd135212_3470.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OMAHSTLEMA.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycAHYD380703:GH2M-3482-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_AERHH
AccessionPrimary (citable) accession number: A0KNV5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: December 12, 2006
Last modified: June 11, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways