Reviewed, UniProtKB/Swiss-Prot A0KNQ8 (PROA_AERHH)

Names and origin

Protein names

Recommended name:
    Gamma-glutamyl phosphate reductase
      Short name=GPR
    EC=1.2.1.41
Alternative name(s):
    Glutamate-5-semialdehyde dehydrogenase
    Glutamyl-gamma-semialdehyde dehydrogenase
      Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	AHA_3420

Organism

Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) [Complete proteome] [HAMAP]

Taxonomic identifier

380703 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate. HAMAP MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_1000049932

Sequences

	Sequence		Length	Mass (Da)
	A0KNQ8-1 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 3D9241D61B0E51CF	FASTA	417	44,328
	10 20 30 40 50 60 MSLEKMGQAA REAAYQLATV STAQKNRALA AIADELEARS HQILAANEKD IAAGRAAGLS 70 80 90 100 110 120 DAMLDRLLLN PARLAGIVAD VRKVISLDDP VGAEIDSRVL ENGLRLSRRR VPIGVIGVIY 130 140 150 160 170 180 EARPNVTIDI ASLCLKTGNA SILRGGRETF HSNLELVRVI QSALAASGLP RDAVQYIDNP 190 200 210 220 230 240 DRALVGELLR LDRYVDMIIP RGGAGLHKMC KENSSIPVII GGFGISHIFV DESADLARSL 250 260 270 280 290 300 AVIDNAKVQR PSACNALDSL LVHEKVAATL LPQLVGLMNE RKVALVATPE AMPLLAGADS 310 320 330 340 350 360 LRAAGPEDFD TEWLSLTLGV KLVADVNEAL AHMREHNAGH SDAILTNDLA NAERFVNGAG 370 380 390 400 410 SAAVYVNAST RFTDGGQFGL GAEVAVSTQM LHARGPMGLT ELTSYKWVGL ADYLSRA

References

[1]

"Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades." Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F. J. Bacteriol. 188:8272-8282(2006) [PubMed: 16980456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000462 Genomic DNA. Translation: ABK38804.1.
RefSeq	YP_857909.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4487146.
GenomeReviews	Gene locus AHA_3420 in contig CP000462_GR.
KEGG	aha:AHA_3420.
TIGR	AHA_3420.
Phylogenomic databases
OMA	A0KNQ8. QYPAACN.
Family and domain databases
HAMAP	MF_00412. [Tree]
InterPro	IPR016163. Ald_DH_C. IPR000965. Gglut_pp_reduct. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
PIRSF	PIRSF000151. GPR. 1 hit.
TIGRFAMs	TIGR00407. proA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_AERHH

Accession

Primary (citable) accession number: A0KNQ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	December 12, 2006
Last modified:	June 16, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)