Reviewed, UniProtKB/Swiss-Prot A0KNL0 (CYSI_AERHH)

Names and origin

Protein names

Recommended name:
    Sulfite reductase [NADPH] hemoprotein beta-component
      Short name=SIR-HP
      Short name=SIRHP
    EC=1.8.1.2

Gene names

Name:	cysI
Ordered Locus Names:	AHA_3372

Organism

Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) [Complete proteome] [HAMAP]

Taxonomic identifier

380703 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	570 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This enzyme catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate By similarity.
Catalytic activity	H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH. HAMAP MF_01540
Cofactor	Binds 1 siroheme per subunit By similarity. Binds 1 4Fe-4S cluster per subunit By similarity.
Subunit structure	Alpha(8)-beta4. The alpha component is a flavoprotein, the beta component is a hemoprotein By similarity.
Sequence similarities	Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	4Fe-4S Heme Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation Inferred from electronic annotation. Source: HAMAP
Cellular component	sulfite reductase complex (NADPH) Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADP or NADPH binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro sulfite reductase (NADPH) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 570	570	Sulfite reductase [NADPH] hemoprotein beta-component HAMAP MF_01540		PRO_0000292957
Sites
	Metal binding	433	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	439	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	478	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	482	1	Iron (siroheme axial ligand) By similarity
	Metal binding	482	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0KNL0-1 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 3F7378CE7FDFC436	FASTA	570	62,961
	10 20 30 40 50 60 MSKQLIPGPV EGPLADNERL KRESDHLRGT IAQDLTDPLT GGFNGDNFQL IRFHGMYQQD 70 80 90 100 110 120 DRDIRPERTA QKLEPLHNVM LRARLPGGII TPAQWQVIDK FAEDHSLYGS IRLTTRQTFQ 130 140 150 160 170 180 FHGVLKRDIK MMHQTLNSTG IDSIATAGDV NRNVLCTSNP VESELHQEAY EWAKKISEHL 190 200 210 220 230 240 LPKTRAYVEI WLDGEKLGGD EEPILGSNYL PRKFKTTVVI PPHNDVDIHA NDLNFVAISD 250 260 270 280 290 300 HGKLVGFNVL VGGGLAMTHG DTSTYPRKAS DFGFVPLSHV LEVAAAVVST QRDWGNRVNR 310 320 330 340 350 360 KNAKTKYTLE RVGVEAFKAE VESRAGIQFG PVRPYEFTSR GDRFGWVEGI DGKHHLTLFI 370 380 390 400 410 420 ENGRLLDFPG KPLKTGMLEI AKVHQGDFRL TANQNLIIAG VPAGEKARIE ALARQYGLLD 430 440 450 460 470 480 DGVSEQRKQS MACVALPTCP LAMAEAERML PAFVTDIEGL LAKHELANDA IIFRVTGCPN 490 500 510 520 530 540 GCGRAMLAEV GLVGKAPGRY NLHLGGNLEG TRIPRLHLEN ITEPQILAEL DALIGRWAKD 550 560 570 RNAGECFGDF VIRAGIIAPV IDSARDFYAA

References

[1]

"Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades." Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F. J. Bacteriol. 188:8272-8282(2006) [PubMed: 16980456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000462 Genomic DNA. Translation: ABK39442.1.
RefSeq	YP_857861.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4490553.
GenomeReviews	Gene locus AHA_3372 in contig CP000462_GR.
KEGG	aha:AHA_3372.
TIGR	AHA_3372.
Phylogenomic databases
OMA	A0KNL0. ITTTQWQ.
Family and domain databases
HAMAP	MF_01540. [Tree]
InterPro	IPR011786. CysI. IPR006066. Nir_Si_BS. IPR006067. Nir_Sir_4Fe4S. IPR005117. NiRdtase/SiRdtase_haem-b_fer. [Graphical view]
Pfam	PF01077. NIR_SIR. 1 hit. PF03460. NIR_SIR_ferr. 2 hits. [Graphical view]
PRINTS	PR00397. SIROHAEM.
TIGRFAMs	TIGR02041. CysI. 1 hit.
PROSITE	PS00365. NIR_SIR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSI_AERHH

Accession

Primary (citable) accession number: A0KNL0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	December 12, 2006
Last modified:	June 16, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)