A0KNI2 (ACSA_AERHH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetyl-coenzyme A synthetase
EC=6.2.1.1

Alternative name(s):
Acetate--CoA ligase
Acyl-activating enzyme

Gene names

Name:	acsA
Ordered Locus Names:	AHA_3344

Organism

Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) [Complete proteome] [HAMAP]

Taxonomic identifier

380703 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	648 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA. HAMAP MF_01123
Post-translational modification	Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme By similarity. HAMAP MF_01123
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	AMP binding Inferred from electronic annotation. Source: InterPro ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetate-CoA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 648	648	Acetyl-coenzyme A synthetase HAMAP MF_01123		PRO_1000065265
Sites
	Active site	516	1	By similarity
Amino acid modifications
	Modified residue	608	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0KNI2 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 73B767E59C45A687	FASTA	648	71,342
	10 20 30 40 50 60 MSESKVYPVK AHISNGALLD KAGYEAMYRA SVQDPDAFWG EQGKILDWMK PYTRVKNTSY 70 80 90 100 110 120 DPGHVSIKWY EDGLLNVSAN CLDRHLAQRG DKVAIIWEGD NPAEDRKLTY RELHTEVCKF 130 140 150 160 170 180 ANVLKAQGVH RGDVVCLYMP MVPEAAIAML ACTRIGAVHS IVFGGFSPEA LAGRIIDSGS 190 200 210 220 230 240 SIVITADEGL RGGRPVPLKK NVDEALTNPE TKVNNVIVLK RTGGNIAWHN HRDIWWHDAV 250 260 270 280 290 300 ATVSADCPPE AMGAEDPLFI LYTSGSTGKP KGVLHTTGGY LVYATLTFKY IFDYHEEDIY 310 320 330 340 350 360 WCTADVGWVT GHSYLVYGPL ANGATTIMFE GVPNYPATNR MSQVVDKHQV SILYTAPTAI 370 380 390 400 410 420 RALMAKGNEA VADTSRSSLR IMGSVGEPIN PEAWEWYYRT IGEERCPIVD TWWQTETGGI 430 440 450 460 470 480 LISPLPGVTD LKPGSATRPF FGVQPALVDN MGEPLEGATE GNLVITDSWP GQMRTVFGDH 490 500 510 520 530 540 ERFEQTYFST FPGRYFTGDG ARRDEDGYYW ITGRVDDVLN VSGHRMGTAE IESALVSHPK 550 560 570 580 590 600 IAEAAVVGVP HEIKGQGIYA YVTLIAGEEP SRELHKEVKE WVRKEIGAIA TPDVIHWAEG 610 620 630 640 LPKTRSGKIM RRILRKIATG ETDSLGDIST LADPGVVDKL IREKSEAA

References

[1]

"Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades." Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F. J. Bacteriol. 188:8272-8282(2006) [PubMed: 16980456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 7966 / NCIB 9240.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000462 Genomic DNA. Translation: ABK37083.1.
RefSeq	YP_857833.1. NC_008570.1.
3D structure databases
ProteinModelPortal	A0KNI2.
SMR	A0KNI2. Positions 7-644.
ModBase	Search...
Protein-protein interaction databases
STRING	A0KNI2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4490124.
GenomeReviews	Gene locus AHA_3344 in contig CP000462_GR.
KEGG	aha:AHA_3344.
PATRIC	20784316. VBIAerHyd135212_3340.
TIGR	AHA_3344.
Phylogenomic databases
eggNOG	COG0365.
HOGENOM	HBG547964.
OMA	LIQWAGG.
PhylomeDB	A0KNI2.
ProtClustDB	PRK00174.
Enzyme and pathway databases
BioCyc	AHYD196024:AHA_3344-MONOMER.
Family and domain databases
HAMAP	MF_01123. Ac_CoA_synth. [Tree]
InterPro	IPR011904. Ac_CoA_lig. IPR024597. Acyl-CoA_synth_DUF3448. IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
KO	K01895.
PANTHER	PTHR24095:SF42. PTHR24095:SF42. 1 hit.
Pfam	PF00501. AMP-binding. 1 hit. PF11930. DUF3448. 1 hit. [Graphical view]
TIGRFAMs	TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACSA_AERHH

Accession

Primary (citable) accession number: A0KNI2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	December 12, 2006
Last modified:	December 14, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program