A0KNF9 (DXS_AERHH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose-5-phosphate synthase
EC=2.2.1.7

Alternative name(s):
1-deoxyxylulose-5-phosphate synthase
Short name=DXP synthase
Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	AHA_3321

Organism

Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) [Complete proteome] [HAMAP]

Taxonomic identifier

380703 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	621 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: EC thiamine pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 621	621	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_1000019001

Sequences

	Sequence		Length	Mass (Da)
	A0KNF9 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: AE80A0AB98B98E77	FASTA	621	67,478
	10 20 30 40 50 60 MSVDISNFPN LALADTPVEL RSLPFERLPV LCNELREYLL RSVSRSSGHL ASGLGTVELT 70 80 90 100 110 120 VALHYVYNTP FDRLVWDVGH QAYPHKILTG RRDRMQSIRQ KDGLHPFPWR GESEYDVLSV 130 140 150 160 170 180 GHSSTSIGAA LGMAVAAESE GLGRKVVAVI GDGAITAGMA FEALNHAGDV HKDMLVVLND 190 200 210 220 230 240 NEMSISENVG ALNNHLARIM SGKLYTTIRE GGKKVLAGLP PVKELAKRAE EHLKGMVVPG 250 260 270 280 290 300 TLFEEFGFNY IGPIDGHDIN ALVETLRNMR NLKGPQLLHV KTKKGKGYEP AEKDPIGYHG 310 320 330 340 350 360 VPKFNPDECT LPKASGGKPS FSAIFGQWLC DMAAKDERLV GITPAMREGS GLVKFSQQYP 370 380 390 400 410 420 DRYFDVAIAE QHAVTFAAGL AIADQKPVVA IYSSFLQRAY DQLIHDVALQ ELPVLFAIDR 430 440 450 460 470 480 AGLVGADGPT HQGAFDISFL RTVPNMVIMT PSDENECRQM LYTGYQCNGP AAVRYPRGSG 490 500 510 520 530 540 TGIQVESEMQ ALALGKGRIV RQGKGTAILA FGTLLQQAKA AAEALDATLV DMRFVKPMDE 550 560 570 580 590 600 ALVFSLAATH DQFVTLEDNA IMGGAGSAVN ELLMRSKQCK PVLNLGLPDR FVEQGTQEEI 610 620 YALLGLDGTG IQRSIEQWLQ A

References

[1]

"Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades." Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F. J. Bacteriol. 188:8272-8282(2006) [PubMed: 16980456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 7966 / NCIB 9240.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000462 Genomic DNA. Translation: ABK37292.1.
RefSeq	YP_857810.1. NC_008570.1.
3D structure databases
ProteinModelPortal	A0KNF9.
SMR	A0KNF9. Positions 3-619.
ModBase	Search...
Protein-protein interaction databases
STRING	A0KNF9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4489901.
GenomeReviews	Gene locus AHA_3321 in contig CP000462_GR.
KEGG	aha:AHA_3321.
PATRIC	20784266. VBIAerHyd135212_3315.
TIGR	AHA_3321.
Phylogenomic databases
eggNOG	COG1154.
HOGENOM	HBG571647.
OMA	DPILYHG.
PhylomeDB	A0KNF9.
ProtClustDB	CLSK2458785.
Enzyme and pathway databases
BioCyc	AHYD196024:AHA_3321-MONOMER.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR011766. TPP_enzyme-bd_C. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR020826. Transketolase_BS. IPR005476. Transketolase_C. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K01662.
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00204. Dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. False negative. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_AERHH

Accession

Primary (citable) accession number: A0KNF9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	December 12, 2006
Last modified:	December 14, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program