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A0KN87

- SYL_AERHH

UniProt

A0KN87 - SYL_AERHH

Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 2 (20 May 2008)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei622 – 6221ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciAHYD380703:GH2M-3246-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
    Alternative name(s):
    Leucyl-tRNA synthetaseUniRule annotation
    Short name:
    LeuRSUniRule annotation
    Gene namesi
    Name:leuSUniRule annotation
    Ordered Locus Names:AHA_3247
    OrganismiAeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240)
    Taxonomic identifieri380703 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeAeromonas
    ProteomesiUP000000756: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 859859Leucine--tRNA ligasePRO_0000334725Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi380703.AHA_3247.

    Structurei

    3D structure databases

    ProteinModelPortaliA0KN87.
    SMRiA0KN87. Positions 228-413.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi42 – 5211"HIGH" regionAdd
    BLAST
    Motifi619 – 6235"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0495.
    HOGENOMiHOG000200747.
    KOiK01869.
    OrthoDBiEOG63Z74X.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_00049_B. Leu_tRNA_synth_B.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 3 hits.
    PF13603. tRNA-synt_1_2. 1 hit.
    [Graphical view]
    PRINTSiPR00985. TRNASYNTHLEU.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A0KN87-1 [UniParc]FASTAAdd to Basket

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    MQEQYVPQSI EPAVQKHWDA KKTFKAVEKV DKEKFYCLSM FPYPSGRLHM    50
    GHVRNYTIGD VISRYQRLNG KNVLQPIGWD AFGLPAENAA IKNNTAPAPW 100
    TYENIEYMKN QLKMLGLGYD WDRELATCKP DYYRWEQWFF TKLYEKGLVY 150
    KKTSSVNWCP NDMTVLANEQ VVDNCCWRCD TPVEQKEIPQ WFIKITDYAE 200
    ELLNDIDNLE GWPEMVKTMQ RNWIGRSEGV NISFAIEGQT EHLEVYTTRP 250
    DTFMGVTYVG IAAGHPLALQ AAESNPELAA FIEECKNTKV AEAELATMEK 300
    KGMATGLYAI HPLDGRKVPV WVANFVLMNY GTGAVMAVPG HDLRDHEFAT 350
    KYGLEIKPVI KPVDGELPNV IDAAYTDKGV LFNSGEFDGL EFQPAFDAIA 400
    SKLESLGCGK RTVNFRLRDW GVSRQRYWGA PIPMLTLADG TVVPTPEDQL 450
    PVLLPEDVVM DGIQSPIKAD AEWAKTTYNG QEAFRETDTF DTFMESSWYY 500
    ARYCSPDYDK GMLDPAAANH WLPVDQYIGG IEHACMHLLY ARFFHKLLRD 550
    AGLVNSDEPF KRLLCQGMVL ADAFYYKDEK GGNVWVSPTD VKVERDEKGR 600
    ITKAVDNEGR EVIHSGMTKM SKSKNNGIDP QLMVERYGAD TVRLFMMFAS 650
    PAEMTLEWSD SGVEGAQRFL RRLWRITFEH VSAGVAPALN VAALSVDQKA 700
    VRRELHKTIA KVSDDVGRRQ TFNTAIAAIM ELMNNLAKLG SDEQDRALMQ 750
    EALEAVVVML YPITPHIGFE LWKMLGKGDD VDHATWPVAD EAAMVETEKL 800
    VVVQINGKMR GKLTVPAEIS QADVEKLAMA DASVQKFTDG LTVRKVIYVP 850
    GKLLNIVAN 859
    Length:859
    Mass (Da):96,865
    Last modified:May 20, 2008 - v2
    Checksum:i07D0C1F52ABAE7E3
    GO

    Sequence cautioni

    The sequence ABK38227.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000462 Genomic DNA. Translation: ABK38227.1. Different initiation.
    RefSeqiYP_857738.1. NC_008570.1.

    Genome annotation databases

    EnsemblBacteriaiABK38227; ABK38227; AHA_3247.
    GeneIDi4490701.
    KEGGiaha:AHA_3247.
    PATRICi20784116. VBIAerHyd135212_3242.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000462 Genomic DNA. Translation: ABK38227.1 . Different initiation.
    RefSeqi YP_857738.1. NC_008570.1.

    3D structure databases

    ProteinModelPortali A0KN87.
    SMRi A0KN87. Positions 228-413.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 380703.AHA_3247.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABK38227 ; ABK38227 ; AHA_3247 .
    GeneIDi 4490701.
    KEGGi aha:AHA_3247.
    PATRICi 20784116. VBIAerHyd135212_3242.

    Phylogenomic databases

    eggNOGi COG0495.
    HOGENOMi HOG000200747.
    KOi K01869.
    OrthoDBi EOG63Z74X.

    Enzyme and pathway databases

    BioCyci AHYD380703:GH2M-3246-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_00049_B. Leu_tRNA_synth_B.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF7. PTHR11946:SF7. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 3 hits.
    PF13603. tRNA-synt_1_2. 1 hit.
    [Graphical view ]
    PRINTSi PR00985. TRNASYNTHLEU.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 7966 / NCIB 9240.

    Entry informationi

    Entry nameiSYL_AERHH
    AccessioniPrimary (citable) accession number: A0KN87
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: May 20, 2008
    Last modified: October 1, 2014
    This is version 62 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3