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A0KN87 (SYL_AERHH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:AHA_3247
OrganismAeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) [Complete proteome] [HAMAP]
Taxonomic identifier380703 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeAeromonas

Protein attributes

Sequence length859 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Sequence caution

The sequence ABK38227.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 859859Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334725

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif619 – 6235"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6221ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A0KN87 [UniParc].

Last modified May 20, 2008. Version 2.
Checksum: 07D0C1F52ABAE7E3

FASTA85996,865
        10         20         30         40         50         60 
MQEQYVPQSI EPAVQKHWDA KKTFKAVEKV DKEKFYCLSM FPYPSGRLHM GHVRNYTIGD 

        70         80         90        100        110        120 
VISRYQRLNG KNVLQPIGWD AFGLPAENAA IKNNTAPAPW TYENIEYMKN QLKMLGLGYD 

       130        140        150        160        170        180 
WDRELATCKP DYYRWEQWFF TKLYEKGLVY KKTSSVNWCP NDMTVLANEQ VVDNCCWRCD 

       190        200        210        220        230        240 
TPVEQKEIPQ WFIKITDYAE ELLNDIDNLE GWPEMVKTMQ RNWIGRSEGV NISFAIEGQT 

       250        260        270        280        290        300 
EHLEVYTTRP DTFMGVTYVG IAAGHPLALQ AAESNPELAA FIEECKNTKV AEAELATMEK 

       310        320        330        340        350        360 
KGMATGLYAI HPLDGRKVPV WVANFVLMNY GTGAVMAVPG HDLRDHEFAT KYGLEIKPVI 

       370        380        390        400        410        420 
KPVDGELPNV IDAAYTDKGV LFNSGEFDGL EFQPAFDAIA SKLESLGCGK RTVNFRLRDW 

       430        440        450        460        470        480 
GVSRQRYWGA PIPMLTLADG TVVPTPEDQL PVLLPEDVVM DGIQSPIKAD AEWAKTTYNG 

       490        500        510        520        530        540 
QEAFRETDTF DTFMESSWYY ARYCSPDYDK GMLDPAAANH WLPVDQYIGG IEHACMHLLY 

       550        560        570        580        590        600 
ARFFHKLLRD AGLVNSDEPF KRLLCQGMVL ADAFYYKDEK GGNVWVSPTD VKVERDEKGR 

       610        620        630        640        650        660 
ITKAVDNEGR EVIHSGMTKM SKSKNNGIDP QLMVERYGAD TVRLFMMFAS PAEMTLEWSD 

       670        680        690        700        710        720 
SGVEGAQRFL RRLWRITFEH VSAGVAPALN VAALSVDQKA VRRELHKTIA KVSDDVGRRQ 

       730        740        750        760        770        780 
TFNTAIAAIM ELMNNLAKLG SDEQDRALMQ EALEAVVVML YPITPHIGFE LWKMLGKGDD 

       790        800        810        820        830        840 
VDHATWPVAD EAAMVETEKL VVVQINGKMR GKLTVPAEIS QADVEKLAMA DASVQKFTDG 

       850 
LTVRKVIYVP GKLLNIVAN 

« Hide

References

[1]"Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades."
Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F.
J. Bacteriol. 188:8272-8282(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 7966 / NCIB 9240.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000462 Genomic DNA. Translation: ABK38227.1. Different initiation.
RefSeqYP_857738.1. NC_008570.1.

3D structure databases

ProteinModelPortalA0KN87.
SMRA0KN87. Positions 228-413.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING380703.AHA_3247.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABK38227; ABK38227; AHA_3247.
GeneID4490701.
KEGGaha:AHA_3247.
PATRIC20784116. VBIAerHyd135212_3242.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycAHYD380703:GH2M-3246-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_AERHH
AccessionPrimary (citable) accession number: A0KN87
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: April 16, 2014
This is version 60 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries