ID   ASTB_AERHH              Reviewed;         445 AA.
AC   A0KMV4;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   12-DEC-2006, sequence version 1.
DT   16-JUN-2009, entry version 15.
DE   RecName: Full=N-succinylarginine dihydrolase;
DE            EC=3.5.3.23;
GN   Name=astB; OrderedLocusNames=AHA_3105;
OS   Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Aeromonadales;
OC   Aeromonadaceae; Aeromonas.
OX   NCBI_TaxID=380703;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16980456; DOI=10.1128/JB.00621-06;
RA   Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J.,
RA   Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M.,
RA   Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F.;
RT   "Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all
RT   trades.";
RL   J. Bacteriol. 188:8272-8282(2006).
CC   -!- FUNCTION: Catalyzes the hydrolysis of N(2)-succinylarginine into
CC       N(2)-succinylornithine, ammonia and CO(2) (By similarity).
CC   -!- CATALYTIC ACTIVITY: N(2)-succinyl-L-arginine + 2 H(2)O = N(2)-
CC       succinyl-L-ornithine + 2 NH(3) + CO(2).
CC   -!- PATHWAY: Amino-acid degradation; L-arginine degradation via AST
CC       pathway; L-glutamate and succinate from L-arginine: step 2/5.
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SIMILARITY: Belongs to the succinylarginine dihydrolase family.
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DR   EMBL; CP000462; ABK38175.1; -; Genomic_DNA.
DR   RefSeq; YP_857605.1; -.
DR   GeneID; 4489032; -.
DR   GenomeReviews; CP000462_GR; AHA_3105.
DR   KEGG; aha:AHA_3105; -.
DR   TIGR; AHA_3105; -.
DR   OMA; A0KMV4; HFAHHPA.
DR   GO; GO:0009015; F:N-succinylarginine dihydrolase activity; IEA:HAMAP.
DR   GO; GO:0019544; P:arginine catabolic process to glutamate; IEA:HAMAP.
DR   HAMAP; MF_01172; -; 1.
DR   InterPro; IPR007079; SuccinylArg_d-Hdrlase_AstB.
DR   Gene3D; G3DSA:3.75.10.20; SuccinylArg_di_hydro; 1.
DR   Pfam; PF04996; AstB; 1.
DR   TIGRFAMs; TIGR03241; arg_catab_astB; 1.
PE   3: Inferred from homology;
KW   Arginine metabolism; Complete proteome; Hydrolase.
FT   CHAIN         1    445       N-succinylarginine dihydrolase.
FT                                /FTId=PRO_1000065713.
FT   REGION       19     28       Substrate binding (By similarity).
FT   REGION      137    138       Substrate binding (By similarity).
FT   ACT_SITE    174    174       By similarity.
FT   ACT_SITE    250    250       By similarity.
FT   ACT_SITE    369    369       Nucleophile (By similarity).
FT   BINDING     110    110       Substrate (By similarity).
FT   BINDING     214    214       Substrate (By similarity).
FT   BINDING     252    252       Substrate (By similarity).
FT   BINDING     363    363       Substrate (By similarity).
SQ   SEQUENCE   445 AA;  48686 MW;  52E4B9CBFC418B75 CRC64;
     MKHFEVNFDG LVGPTHNYAG LSYGNVASQN NAKEASNPKE AAKQGLRKMK ALTELGMTQG
     VLAPQERPDL ATLRRLGFTG NDASVLAQAA KQAPAVLAAC YSASSMWTAN AATVSPSADT
     QDGRIHFTPA NLTNKFHRSL EPEVTGRILR AVFNNDRHFY HHQHLPENDH FGDEGAANHT
     RLCRAYGESG VELFVYGRSA FDVSQPAPKR YPARQTLEAS QAIARLHGLG DESAVFIQQN
     PDVIDQGVFH NDVIAVGNQN VLFFHQQAFL NTASALAEVR TKFGDGELHF IEVPTAEVSV
     QDAVKSYLFN TQILTLPSGE MAIIAPTECR DNPAVSAYLT KLVTLGTPIK GVHYMDVKQS
     MRNGGGPACL RLRVAMNDTE LAAVNPACLI TDSQFARLDG WVDRHYRDSL ALDDLRDPAL
     VMESRTALDE LTQILKLGSV YPFQR
//