A0KMM4 (ACEK_AERHH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isocitrate dehydrogenase kinase/phosphatase
Short name=IDH kinase/phosphatase
Short name=IDHK/P
EC=2.7.11.5
EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	AHA_3021

Organism

Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) [Complete proteome] [HAMAP]

Taxonomic identifier

380703 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	575 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity. HAMAP MF_00747
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity HAMAP MF_00747.
Sequence similarities	Belongs to the AceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: EC phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 575	575	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_0000288279
Regions
	Nucleotide binding	315 – 321	7	ATP By similarity
Sites
	Active site	371	1	By similarity
	Binding site	336	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0KMM4 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: DFFCBAA9E00F527C	FASTA	575	67,157
	10 20 30 40 50 60 MQQPLEQAVA ETILQRFESF YSRFLEITQG SKSRFENSDW LGVQLAGRER IRLYDHHVGA 70 80 90 100 110 120 TTALIKQMMG ATLPSQALLK QVKGAFSDLL PKCENFEVAE SFFNSVYRRI FRHRNIRDEN 130 140 150 160 170 180 LYIHPFRSRG EHPDLSALLR IYRTDLAHLP QTLSQLLGDY SFTLPYEDKQ RDIVDIQRHL 190 200 210 220 230 240 AENGPAILHD EPFAIELLKE VFYRNKGAYL VGLIRVKGQV FPFILPLLST GQSIYVDTVI 250 260 270 280 290 300 FEPELASIVF GFARAYFMVY APMPALFVLF LRQIMPHKPD YEIYNAIGCQ KHGKTELYRH 310 320 330 340 350 360 YQQHLAQSRE QFVIAPGIKG MVMSVFTLPS YDVVFKVIKD EFTPPKDVSH EQVKAKYRLV 370 380 390 400 410 420 KQHDRVGRMA DTQEFTNFEF PLDRISPELL AELKTVAPSA LTLTDDKLVI KHLYTERKMI 430 440 450 460 470 480 PLNLYLDKAD EQQTRLALEE YGNAIKQLAA ANIFPGDMLF KNFGVTRHGR VVFYDYDEIC 490 500 510 520 530 540 YMTECNFRQI PPPRYPEDEW SAEPWYSVAP NDIFPEEFAT FLLQKPQVRD IMMQLHKELF 550 560 570 DANYWKMLQS NIKEGVFEDV YPYRRKKRFK FQRGG

References

[1]

"Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades." Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F. J. Bacteriol. 188:8272-8282(2006) [PubMed: 16980456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 7966 / NCIB 9240.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000462 Genomic DNA. Translation: ABK39426.1.
RefSeq	YP_857525.1. NC_008570.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A0KMM4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4489046.
GenomeReviews	Gene locus AHA_3021 in contig CP000462_GR.
KEGG	aha:AHA_3021.
PATRIC	20783654. VBIAerHyd135212_3023.
TIGR	AHA_3021.
Phylogenomic databases
eggNOG	COG4579.
HOGENOM	HBG298593.
OMA	RMTPLNI.
PhylomeDB	A0KMM4.
ProtClustDB	PRK02946.
Enzyme and pathway databases
BioCyc	AHYD196024:AHA_3021-MONOMER.
Family and domain databases
HAMAP	MF_00747. AceK. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
KO	K00906.
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProDom	PD043552. Isocitrate_DH_AceK. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

ACEK_AERHH

Accession

Primary (citable) accession number: A0KMM4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	December 12, 2006
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program