A0KM24 (PUR5_AERHH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine cyclo-ligase
EC=6.3.3.1

Alternative name(s):
AIR synthase
AIRS
Phosphoribosyl-aminoimidazole synthetase

Gene names

Name:	purM
Ordered Locus Names:	AHA_2821

Organism

Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) [Complete proteome] [HAMAP]

Taxonomic identifier

380703 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP MF_00741_B
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP MF_00741_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00741_B.
Sequence similarities	Belongs to the AIR synthase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine cyclo-ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	Phosphoribosylformylglycinamidine cyclo-ligase HAMAP MF_00741_B		PRO_1000046417

Sequences

	Sequence		Length	Mass (Da)
	A0KM24 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: B20B308F4F4C3490	FASTA	345	36,759
	10 20 30 40 50 60 MTDKTSLSYK DAGVDIDAGN ALVERIKGVS KRTRRPEVLG GLGGFGALCQ IPAGYKEPVL 70 80 90 100 110 120 VSGTDGVGTK LRLAIDLKKH DTVGIDLVAM CVNDLIVQGA EPLFFLDYYA TGKLDVDTAA 130 140 150 160 170 180 AVVTGIGAGC EQSGCALVGG ETAEMPGMYE GEDYDIAGFC VGVVEKSEII DGSKVGEGDA 190 200 210 220 230 240 LIALAASGPH SNGFSLVRKI LEVSKADVQQ PLGDTTLANA LLEPTRIYVK PVLKLIKECE 250 260 270 280 290 300 IHALSHITGG GFWENIPRVL PANTQAVIDE QSWQWPAVFS WLQQAGNVTR HEMYRTFNCG 310 320 330 340 VGMIIALPAD QLEKALTLLK TEGENAWHIG YITKAADGEE QVIIQ

References

[1]

"Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades." Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F. J. Bacteriol. 188:8272-8282(2006) [PubMed: 16980456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 7966 / NCIB 9240.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000462 Genomic DNA. Translation: ABK37366.1.
RefSeq	YP_857325.1. NC_008570.1.
3D structure databases
ProteinModelPortal	A0KM24.
SMR	A0KM24. Positions 5-345.
ModBase	Search...
Protein-protein interaction databases
STRING	A0KM24.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4488746.
GenomeReviews	Gene locus AHA_2821 in contig CP000462_GR.
KEGG	aha:AHA_2821.
PATRIC	20783270. VBIAerHyd135212_2831.
TIGR	AHA_2821.
Phylogenomic databases
eggNOG	COG0150.
HOGENOM	HBG531222.
OMA	PENIPRV.
PhylomeDB	A0KM24.
ProtClustDB	PRK05385.
Enzyme and pathway databases
BioCyc	AHYD196024:AHA_2821-MONOMER.
Family and domain databases
HAMAP	MF_00741_B. AIRS_B. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR004733. PurM_cligase. IPR016188. PurM_N-like. [Graphical view]
KO	K01933.
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 1 hit. SSF55326. PurM_N-like. 1 hit.
TIGRFAMs	TIGR00878. PurM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR5_AERHH

Accession

Primary (citable) accession number: A0KM24

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	December 12, 2006
Last modified:	December 14, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program