A0KLC8 (ARGA_AERHH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Amino-acid acetyltransferase
EC=2.3.1.1

Alternative name(s):
N-acetylglutamate synthase
Short name=AGS
Short name=NAGS

Gene names

Name:	argA
Ordered Locus Names:	AHA_2569

Organism

Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) [Complete proteome] [HAMAP]

Taxonomic identifier

380703 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	442 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate. HAMAP MF_01105
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4. HAMAP MF_01105
Subcellular location	Cytoplasm By similarity HAMAP MF_01105.
Miscellaneous	In bacteria which possess the bifunctional enzyme ornithine acetyltransferase/N-acetylglutamate synthase (ArgJ), ArgA fulfills an anaplerotic role. HAMAP MF_01105
Sequence similarities	Belongs to the acetyltransferase family. ArgA subfamily. Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetyl-CoA:L-glutamate N-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 442	442	Amino-acid acetyltransferase HAMAP MF_01105		PRO_1000084809
Regions
	Domain	295 – 442	148	N-acetyltransferase

Sequences

	Sequence		Length	Mass (Da)
	A0KLC8 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: A5CBF47944BDE782	FASTA	442	49,230
	10 20 30 40 50 60 MREREPSLVY AFRQSTPYVN VHRGATFVLM MGGEALCHPN FANIVSDIAL LQTLGIRLVL 70 80 90 100 110 120 VFGSRPQNDE ALARAGIEAQ YHKRIRVTDD ESFAIIKQVC GGLQYDITAQ LSMGLANTPM 130 140 150 160 170 180 QGARISVVSG NFVTAQPLGV DDGIDFCHSG RVRRIDVEGI TRQLDQKSLV LISPIGCSVT 190 200 210 220 230 240 GESFNLSSEE VARRVAVDLK ADKLICFSST QGVMDRHGEA ISELFPEQAE ELLVELEQAG 250 260 270 280 290 300 EEMSGTARYL RAAIASCRGG VPRSHLVSYQ DDGAMLQELF SRDGLGTQIV RESAEQARAA 310 320 330 340 350 360 TIEDIGGILD LIRPLEEEGI LVRRSREQLE MEIDKFTIIE RDGLIIGCAA LYCFMEEAMA 370 380 390 400 410 420 EMACVAIHPD YRNSNRGDQL VAKVAERAKR LGIRRLFVLT TRSIHWFRER GFDPLEVEDL 430 440 PVARQRLYNW QRRSKVLSKT IV

References

[1]

"Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades." Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F. J. Bacteriol. 188:8272-8282(2006) [PubMed: 16980456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 7966 / NCIB 9240.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000462 Genomic DNA. Translation: ABK37645.1.
RefSeq	YP_857079.1. NC_008570.1.
3D structure databases
ProteinModelPortal	A0KLC8.
ModBase	Search...
Protein-protein interaction databases
STRING	A0KLC8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4489709.
GenomeReviews	Gene locus AHA_2569 in contig CP000462_GR.
KEGG	aha:AHA_2569.
PATRIC	20782730. VBIAerHyd135212_2567.
TIGR	AHA_2569.
Phylogenomic databases
eggNOG	COG0548.
HOGENOM	HBG545264.
OMA	AFNLAME.
PhylomeDB	A0KLC8.
ProtClustDB	PRK05279.
Enzyme and pathway databases
BioCyc	AHYD196024:AHA_2569-MONOMER.
Family and domain databases
HAMAP	MF_01105. N-acetyl_glu_synth. [Tree]
InterPro	IPR000182. AcTrfase_GCN5-related_dom. IPR016181. Acyl_CoA_acyltransferase. IPR001048. Asp/Glu/Uridylate_kinase. IPR010167. NH2A_AcTrfase_ArgA. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit. G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 1 hit.
KO	K14682.
Pfam	PF00696. AA_kinase. 1 hit. PF00583. Acetyltransf_1. 1 hit. [Graphical view]
PIRSF	PIRSF000423. ArgA. 1 hit.
SUPFAM	SSF53633. Aa_kinase. 1 hit. SSF55729. Acyl_CoA_acyltransferase. 1 hit.
TIGRFAMs	TIGR01890. N-Ac-Glu-synth. 1 hit.
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARGA_AERHH

Accession

Primary (citable) accession number: A0KLC8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	December 12, 2006
Last modified:	December 14, 2011
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program