Reviewed, UniProtKB/Swiss-Prot A0KL51 (HEMH_AERHH)

Names and origin

Protein names

Recommended name:
    Ferrochelatase
    EC=4.99.1.1
Alternative name(s):
    Protoheme ferro-lyase
    Heme synthetase

Gene names

Name:	hemH
Ordered Locus Names:	AHA_2488

Organism

Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) [Complete proteome] [HAMAP]

Taxonomic identifier

380703 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the ferrous insertion into protoporphyrin IX. HAMAP MF_00323
Catalytic activity	Protoheme + 2 H+ = protoporphyrin + Fe2+. HAMAP MF_00323
Pathway	Porphyrin metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1. HAMAP MF_00323
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ferrochelatase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferrochelatase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	Ferrochelatase HAMAP MF_00323		PRO_1000072021
Sites
	Metal binding	214	1	Iron By similarity
	Metal binding	295	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0KL51-1 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 9AC57183CBD381CC	FASTA	341	38,525
	10 20 30 40 50 60 MGPFLHSDIT TERQEAYVTT KTGILLVNLG TPAAPTTAAV KAFLSQFLHD QRVVDLPRYL 70 80 90 100 110 120 WCPLLHFIIL PTRSPKVAKL YQQVWTEQGS PLMVISKQQR AALEQELKRE GVEVPVELAM 130 140 150 160 170 180 TYGSPSLDEG WQALKAKGVN RVILLPLYPQ YSVSTTASVF DGWAKAMKKE RNLPVVRLIR 190 200 210 220 230 240 DYHAHPEYIQ ALAMSVRRHW EQHGQGEHLL MSFHGIPERY EREGDPYGHQ CRRTAALLAE 250 260 270 280 290 300 VLGLEASQWT ASFQSRFGKE EWLKPYTDVT IAGLPATGIK RLDVICPAFA ADCLETLEEI 310 320 330 340 QVQNREIFMA AGGEQFEYIP ALNSDQAHIR MMVSLICREL A

References

[1]

"Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades." Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F. J. Bacteriol. 188:8272-8282(2006) [PubMed: 16980456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000462 Genomic DNA. Translation: ABK38925.1.
RefSeq	YP_857002.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A0KL51.
Genome annotation databases
GeneID	4490789.
GenomeReviews	Gene locus AHA_2488 in contig CP000462_GR.
KEGG	aha:AHA_2488.
TIGR	AHA_2488.
Phylogenomic databases
OMA	TIEEIGM.
Family and domain databases
HAMAP	MF_00323. [Tree]
InterPro	IPR001015. Ferrochelatase. IPR019772. Ferrochelatase_AS. [Graphical view]
PANTHER	PTHR11108. Ferrochelatase. 1 hit.
Pfam	PF00762. Ferrochelatase. 1 hit. [Graphical view]
ProDom	PD002792. Ferrochelatase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00109. hemH. 1 hit.
PROSITE	PS00534. FERROCHELATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMH_AERHH

Accession

Primary (citable) accession number: A0KL51

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	December 12, 2006
Last modified:	November 3, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)