Reviewed, UniProtKB/Swiss-Prot A0KKP5 (SYFA_AERHH)

Names and origin

Protein names

Recommended name:
    Phenylalanyl-tRNA synthetase alpha chain
    EC=6.1.1.20
Alternative name(s):
    Phenylalanine--tRNA ligase alpha chain
      Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	AHA_2323

Organism

Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) [Complete proteome] [HAMAP]

Taxonomic identifier

380703 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity.
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00281
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 327	327	Phenylalanyl-tRNA synthetase alpha chain HAMAP MF_00281		PRO_1000006794
Sites
	Metal binding	252	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0KKP5-1 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: D1FA3FB2BDA941AF	FASTA	327	37,185
	10 20 30 40 50 60 MQQLEEVVGQ ARAEIEGVSD IAALDEIRVK YLGKKGFFTE QMKGLGALSA EERPAAGAVI 70 80 90 100 110 120 NQAKQQVQDA LNERREALEI AVLNQKLAAE TIDVSLPGRR IENGGLHPVT RTIERIERLF 130 140 150 160 170 180 GEMGFKVARG PEIEDGFHNF DALNIPAHHP ARTDHDTFYF NPDLMLRTHT SGVQIRTMEH 190 200 210 220 230 240 QQPPIRIIAP GRVYRNDYDM THTPMFHQVE GLLVDEHASF TELKGILHDF LRNYFEEDLT 250 260 270 280 290 300 IRFRPSYFPF TEPSAEVDVM GKNGKWLEVL GCGMVHPNVL RSVGIDPEKY SGFAFGMGVE 310 320 RLTMLRYGVN DLRAFFENDL RFLKQFK

References

[1]

"Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades." Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F. J. Bacteriol. 188:8272-8282(2006) [PubMed: 16980456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000462 Genomic DNA. Translation: ABK37031.1.
RefSeq	YP_856846.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A0KKP5.
Genome annotation databases
GeneID	4487780.
GenomeReviews	Gene locus AHA_2323 in contig CP000462_GR.
KEGG	aha:AHA_2323.
TIGR	AHA_2323.
Phylogenomic databases
OMA	FRASYFP.
Family and domain databases
HAMAP	MF_00281. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II_cons-reg. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_synth_II_N. IPR002319. Phenylalanyl-tRNA_Synthase_acu. [Graphical view]
PANTHER	PTHR11538. tRNA-synt_2d. 1 hit.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
TIGRFAMs	TIGR00468. pheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_AERHH

Accession

Primary (citable) accession number: A0KKP5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	December 12, 2006
Last modified:	November 3, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)