Reviewed, UniProtKB/Swiss-Prot A0KKK9 (PYRD_AERHH)

Names and origin

Protein names

Recommended name:
    Dihydroorotate dehydrogenase
    EC=1.3.3.1
Alternative name(s):
    Dihydroorotate oxidase
    DHOdehase
      Short name=DHODase
      Short name=DHOD

Gene names

Name:	pyrD
Ordered Locus Names:	AHA_2287

Organism

Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) [Complete proteome] [HAMAP]

Taxonomic identifier

380703 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + O2 = orotate + H2O2. HAMAP MF_00225
Cofactor	Binds 1 FMN per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 4/6. HAMAP MF_00225
Subunit structure	Homodimer By similarity.
Subcellular location	Cell membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Cell membrane Membrane
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro UMP biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydroorotate oxidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	Dihydroorotate dehydrogenase HAMAP MF_00225		PRO_1000024147
Sites
	Active site	175	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0KKK9-1 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: C2D036246386BE8D	FASTA	336	36,360
	10 20 30 40 50 60 MLYPLARHFL FKLNPEQAHD LSIKYLPRLL GTPLDCFFRH SLPKRPVTVM GLSFANPVGL 70 80 90 100 110 120 AAGLDKDGEC IDAFGAMGFG FIEVGTVTPR PQSGNDKPRL FRVIPAEGII NRMGFNNKGV 130 140 150 160 170 180 DHLVAQVKKA KYQGVIGINI GKNKDTPIEQ GKDDYLICMD KVYDQAGYIA VNISSPNTPG 190 200 210 220 230 240 LRSLQYGDAL DELLAALKVR QQELAAQYKK YVPLAVKIAP DLTPDEINQV AASLIKNGID 250 260 270 280 290 300 GVIATNTTLE RDMIYDMPHA AEAGGLSGRP LQHKSTEVIR QLAKALDGAL PIIGVGGIDS 310 320 330 AMAAREKLAA GASLVQIYSG FIYKGPDLVK EIVTHI

References

[1]

"Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades." Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F. J. Bacteriol. 188:8272-8282(2006) [PubMed: 16980456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000462 Genomic DNA. Translation: ABK37752.1.
RefSeq	YP_856810.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4488940.
GenomeReviews	Gene locus AHA_2287 in contig CP000462_GR.
KEGG	aha:AHA_2287.
TIGR	AHA_2287.
Phylogenomic databases
OMA	A0KKK9. NAEQQGG.
Family and domain databases
HAMAP	MF_00225. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR012135. Dihydroorotate_DH_1_2. IPR005719. Dihydroorotate_DH_2. IPR001295. Dihydroorotate_DH_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01180. DHO_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMs	TIGR01036. pyrD_sub2. 1 hit.
PROSITE	PS00911. DHODEHASE_1. 1 hit. PS00912. DHODEHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRD_AERHH

Accession

Primary (citable) accession number: A0KKK9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	December 12, 2006
Last modified:	June 16, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)