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Protein

Ribosomal RNA small subunit methyltransferase F

Gene

rsmF

Organism
Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + cytosine(1407) in 16S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(1407) in 16S rRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei149 – 1491S-adenosyl-L-methionineUniRule annotation
Binding sitei176 – 1761S-adenosyl-L-methionineUniRule annotation
Binding sitei194 – 1941S-adenosyl-L-methionineUniRule annotation
Active sitei247 – 2471NucleophileUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciAHYD380703:GH2M-2262-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase FUniRule annotation (EC:2.1.1.178UniRule annotation)
Alternative name(s):
16S rRNA m5C1407 methyltransferaseUniRule annotation
rRNA (cytosine-C(5)-)-methyltransferase RsmFUniRule annotation
Gene namesi
Name:rsmFUniRule annotation
Ordered Locus Names:AHA_2262
OrganismiAeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240)
Taxonomic identifieri380703 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeAeromonas
Proteomesi
  • UP000000756 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 475475Ribosomal RNA small subunit methyltransferase FPRO_0000284995Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi380703.AHA_2262.

Structurei

3D structure databases

ProteinModelPortaliA0KKI5.
SMRiA0KKI5. Positions 6-473.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni125 – 1317S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CYJ. Bacteria.
COG0144. LUCA.
COG3270. LUCA.
HOGENOMiHOG000218115.
KOiK11392.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01579. 16SrRNA_methyltr_F. 1 hit.
InterProiIPR018314. Fmu/NOL1/Nop2p_CS.
IPR031341. Methyltr_RsmF_N.
IPR001678. MeTrfase_RsmB/NOP2.
IPR027391. Nol1_Nop2_Fmu_2.
IPR011023. Nop2p.
IPR023267. RCMT.
IPR023545. rRNA_ssu_MeTfrase_F.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01189. Methyltr_RsmB-F. 1 hit.
PF17125. Methyltr_RsmF_N. 1 hit.
PF13636. Methyltranf_PUA. 1 hit.
[Graphical view]
PRINTSiPR02008. RCMTFAMILY.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00446. nop2p. 1 hit.
PROSITEiPS01153. NOL1_NOP2_SUN. 1 hit.
PS51686. SAM_MT_RSMB_NOP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0KKI5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHDNTYLPDH FLRHIAAIMP AHLSMDEFVA SCRRPLRRSI RVNTLKISVA
60 70 80 90 100
AFVARMQPLG WQLDPVPWCD TGFWLSREDE SVPLGNTAEH LSGLFYIQEA
110 120 130 140 150
SSMLPVTALF ACEQTRRDGM LLDAAAAPGS KTTQIAALMN NQGMLVANEY
160 170 180 190 200
SSSRLKVLSA NLQRCGVTNV GMTHFDAKVF GQWLPETFDA ILLDAPCSGE
210 220 230 240 250
GSVRKDEDAL RNWSIESIDE IAAVQQGLLE SAFHALKPGG VLVYSTCTLS
260 270 280 290 300
LQENQAVCQS LLDKFGAAFS FDSLADLFPA AEQACTPEGY LHVWPQIFDS
310 320 330 340 350
EGFFVARLRK HYSVPNTMFK PGKLGKFPFL PLPAKESEPM LREIEAAFGV
360 370 380 390 400
VPQGNLFGRS EEIWLFPQRF EQVQGKLRFD RIGLKLAETF KKGYRLTHEW
410 420 430 440 450
ALAYGDGASK GFVELGIADA REFMMGRDVW PEQAAGTGEV IVRYQGHTLG
460 470
MGKWVGSRVK NALPRELVRD NNLFV
Length:475
Mass (Da):52,829
Last modified:May 1, 2007 - v2
Checksum:i1A77117C5CB03B96
GO

Sequence cautioni

The sequence ABK39873 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000462 Genomic DNA. Translation: ABK39873.1. Different initiation.
RefSeqiWP_011706116.1. NC_008570.1.
YP_856786.1. NC_008570.1.

Genome annotation databases

EnsemblBacteriaiABK39873; ABK39873; AHA_2262.
GeneIDi4488729.
KEGGiaha:AHA_2262.
PATRICi20782092. VBIAerHyd135212_2261.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000462 Genomic DNA. Translation: ABK39873.1. Different initiation.
RefSeqiWP_011706116.1. NC_008570.1.
YP_856786.1. NC_008570.1.

3D structure databases

ProteinModelPortaliA0KKI5.
SMRiA0KKI5. Positions 6-473.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi380703.AHA_2262.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK39873; ABK39873; AHA_2262.
GeneIDi4488729.
KEGGiaha:AHA_2262.
PATRICi20782092. VBIAerHyd135212_2261.

Phylogenomic databases

eggNOGiENOG4105CYJ. Bacteria.
COG0144. LUCA.
COG3270. LUCA.
HOGENOMiHOG000218115.
KOiK11392.

Enzyme and pathway databases

BioCyciAHYD380703:GH2M-2262-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01579. 16SrRNA_methyltr_F. 1 hit.
InterProiIPR018314. Fmu/NOL1/Nop2p_CS.
IPR031341. Methyltr_RsmF_N.
IPR001678. MeTrfase_RsmB/NOP2.
IPR027391. Nol1_Nop2_Fmu_2.
IPR011023. Nop2p.
IPR023267. RCMT.
IPR023545. rRNA_ssu_MeTfrase_F.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01189. Methyltr_RsmB-F. 1 hit.
PF17125. Methyltr_RsmF_N. 1 hit.
PF13636. Methyltranf_PUA. 1 hit.
[Graphical view]
PRINTSiPR02008. RCMTFAMILY.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00446. nop2p. 1 hit.
PROSITEiPS01153. NOL1_NOP2_SUN. 1 hit.
PS51686. SAM_MT_RSMB_NOP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRSMF_AERHH
AccessioniPrimary (citable) accession number: A0KKI5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: May 1, 2007
Last modified: September 7, 2016
This is version 64 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.