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A0KKF6 (SYM_AERHH) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Methionine--tRNA ligase
EC=6.1.1.10
Alternative name(s):
Methionyl-tRNA synthetase
Short name=MetRS
Gene names
Name:metG
Ordered Locus Names:AHA_2233
OrganismAeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) [Complete proteome] [HAMAP]
Taxonomic identifier380703 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length677 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP MF_00098

Catalytic activity

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP MF_00098

Subunit structure

Homodimer By similarity. HAMAP MF_00098

Subcellular location

Cytoplasm By similarity HAMAP MF_00098.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.

Contains 1 tRNA-binding domain.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
RNA-binding
Zinc
tRNA-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processmethionyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

methionine-tRNA ligase activity

Inferred from electronic annotation. Source: EC

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 677677Methionine--tRNA ligase HAMAP MF_00098
PRO_0000331775

Regions

Domain576 – 677102tRNA-binding
Motif15 – 2511"HIGH" region HAMAP MF_00098
Motif333 – 3375"KMSKS" region HAMAP MF_00098

Sites

Metal binding1461Zinc By similarity
Metal binding1491Zinc By similarity
Metal binding1591Zinc By similarity
Metal binding1621Zinc By similarity
Binding site3361ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A0KKF6 [UniParc].

Last modified December 12, 2006. Version 1.
Checksum: 140774078EAAA43E

FASTA67775,679
        10         20         30         40         50         60 
MATDPRTMLV TCALPYANGS IHLGHMLEHI QADIWVRYQR MRGHQVHFVC ADDAHGTPIM 

        70         80         90        100        110        120 
LKAQQLGITP EEMIAAVSKE HQADFAGFNI SFDNYHSTHS DENRELAELI YGRLQAGGKI 

       130        140        150        160        170        180 
KSRTISQLFD PEKSMFLPDR FVKGTCPKCK SPEQYGDNCD SCGATYSPTE LIDPKSAVSG 

       190        200        210        220        230        240 
ATPVMKDSEH FFFDLPQFEK WLAEWVRGSG AIQEEMANKM QEWFESGLQQ WDITRDAPYF 

       250        260        270        280        290        300 
GFEIPGAPGK YFYVWLDAPI GYMASFKNLC NKRRDIDFDS YWKADSEAEL YHFIGKDIAY 

       310        320        330        340        350        360 
FHCLFWPSML EGAGFRKPTK VNVHGYVTVN GAKMSKSKGT FIKASTYLNH LDPECLRYYY 

       370        380        390        400        410        420 
AAKLNSRIDD LDLNLDDFVA RVNADVVNKL VNLASRNAGF IAKRFDGKLA ATCAEPELYA 

       430        440        450        460        470        480 
EFANSRTAIA EAYESREFSR AIREIMALAD KANRYVDDKA PWVIAKQEGA DAELQAVCSV 

       490        500        510        520        530        540 
GINLFRVLMA YLKPVMPLLA ERAEAFLAET LSWDGIEMPL VDHTVAPFKA LFSRIEPAKI 

       550        560        570        580        590        600 
EAMIDASKED LAKEQAPKAS GPLVDDPISE TISYDDFAKI DLRVALIKKA EAVPEADKLL 

       610        620        630        640        650        660 
KLQLDIGGET RQVFAGIKSA YNPEDLEGKL TVMVANLAPR KMRFGMSEGM VLAAGPGGKD 

       670 
LWILEPQEGA KPGMRVK

« Hide

References

[1]"Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades."
Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F.
J. Bacteriol. 188:8272-8282(2006) [PubMed: 16980456] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 7966 / NCIB 9240.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000462 Genomic DNA. Translation: ABK39655.1.
RefSeqYP_856757.1. NC_008570.1.

3D structure databases

ProteinModelPortalA0KKF6.
SMRA0KKF6. Positions 6-549.
ModBaseSearch...

Protein-protein interaction databases

STRINGA0KKF6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4489272.
GenomeReviewsGene locus AHA_2233 in contig CP000462_GR.
KEGGaha:AHA_2233.
PATRIC20782036. VBIAerHyd135212_2233.
TIGRAHA_2233.

Phylogenomic databases

eggNOGCOG0143.
HOGENOMHBG721667.
OMAEEKTRNV.
PhylomeDBA0KKF6.
ProtClustDBPRK00133.

Enzyme and pathway databases

BioCycAHYD196024:AHA_2233-MONOMER.

Family and domain databases

HAMAPMF_00098. Met_tRNA_synth_type1.
[Tree]
InterProIPR015413. aa-tRNA-synt_I.
IPR001412. aa-tRNA-synth_I_CS.
IPR004495. Met-tRNA-synth_Ia_bsu_C.
IPR014758. Met-tRNA_synth.
IPR023458. Met-tRNA_synth_1.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KOK01874.
PfamPF08264. Anticodon_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSPR01041. TRNASYNTHMET.
SUPFAMSSF50249. Nucleic_acid_OB. 1 hit.
SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMsTIGR00398. MetG. 1 hit.
TIGR00399. MetG_C_term. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYM_AERHH
AccessionPrimary (citable) accession number: A0KKF6
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: December 12, 2006
Last modified: January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

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