A0KJS0 (GUAA_AERHH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP synthase [glutamine-hydrolyzing]
EC=6.3.5.2

Alternative name(s):
GMP synthetase
Glutamine amidotransferase

Gene names

Name:	guaA
Ordered Locus Names:	AHA_1990

Organism

Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) [Complete proteome] [HAMAP]

Taxonomic identifier

380703 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	529 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_00344
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_00344
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_00344
Subunit structure	Homodimer By similarity. HAMAP MF_00344
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain. Contains 1 GMPS ATP-PPase (ATP pyrophosphatase) domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 529	529	GMP synthase [glutamine-hydrolyzing] HAMAP MF_00344		PRO_1000120203
Regions
	Domain	9 – 211	203	Glutamine amidotransferase type-1
	Domain	212 – 404	193	GMPS ATP-PPase
	Nucleotide binding	239 – 245	7	ATP By similarity
Sites
	Active site	86	1	Nucleophile By similarity
	Active site	185	1	By similarity
	Active site	187	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0KJS0 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: E53AF9F903C41310	FASTA	529	58,834
	10 20 30 40 50 60 MTKDIHQHRI LILDFGSQYT QLIARRVREI GVYCELWAWD VTEEQMREFN PSGIILSGGP 70 80 90 100 110 120 ESVTEAGSPR APEYVFNAGV PVFGICYGMQ TMAEQLGGKV QSSTEREFGY AKVEVLGNSG 130 140 150 160 170 180 KTSALLRNIE DAIADNGNAL LDVWMSHGDK VTTIPADFTT IAQTATCPHA AMANEAKRFY 190 200 210 220 230 240 GVQFHPEVTH TRQGARMLEH FVKDICGCEC LWTPATIIDD AVARIREQVG DDEVILGLSG 250 260 270 280 290 300 GVDSSVVAML VHRAIGDRLT CVFVDNGLLR LNEGQQVMEM FGDHFGLNIV KVDAEERFLS 310 320 330 340 350 360 ALAGEDEPEA KRKIIGRVFV EVFDDEAKKL KNARWLAQGT IYPDVIESAA SKTGKAHVIK 370 380 390 400 410 420 SHHNVGGLPE EMKMGLVEPL RELFKDEVRR VGLELGLPYD MLYRHPFPGP GLGVRVLGEV 430 440 450 460 470 480 KKEYCDLLRK ADAVFIEELR KADLYNQVSQ AFAVFLPVRS VGVMGDGRKY DWVIALRAVE 490 500 510 520 TIDFMTAHWA HLPYDFLGHV SNRIINEING ISRVVYDVSG KPPATIEWE

References

[1]

"Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades." Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F. J. Bacteriol. 188:8272-8282(2006) [PubMed: 16980456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 7966 / NCIB 9240.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000462 Genomic DNA. Translation: ABK36969.1.
RefSeq	YP_856521.1. NC_008570.1.
3D structure databases
ProteinModelPortal	A0KJS0.
SMR	A0KJS0. Positions 3-529.
ModBase	Search...
Protein-protein interaction databases
STRING	A0KJS0.
Protein family/group databases
MEROPS	C26.A17.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4489436.
GenomeReviews	Gene locus AHA_1990 in contig CP000462_GR.
KEGG	aha:AHA_1990.
PATRIC	20781580. VBIAerHyd135212_2011.
TIGR	AHA_1990.
Phylogenomic databases
eggNOG	COG0519.
HOGENOM	HBG391775.
OMA	DGRTYEY.
PhylomeDB	A0KJS0.
ProtClustDB	PRK00074.
Enzyme and pathway databases
BioCyc	AHYD196024:AHA_1990-MONOMER.
Family and domain databases
HAMAP	MF_00344. GMP_synthase. [Tree]
InterPro	IPR017926. GATASE_1. IPR001674. GMP_synth_C. IPR004739. GMP_synth_N. IPR022955. GMP_synthase. IPR022310. NAD/GMP_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01951.
Pfam	PF00117. GATase. 1 hit. PF00958. GMP_synt_C. 1 hit. PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00884. GuaA_Cterm. 1 hit. TIGR00888. GuaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. PS51553. GMPS_ATP_PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAA_AERHH

Accession

Primary (citable) accession number: A0KJS0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	December 12, 2006
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program