Reviewed, UniProtKB/Swiss-Prot A0KJP1 (DADA_AERHH)

Names and origin

Protein names

Recommended name:
    D-amino acid dehydrogenase small subunit
    EC=1.4.99.1

Gene names

Name:	dadA
Ordered Locus Names:	AHA_1961

Organism

Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) [Complete proteome] [HAMAP]

Taxonomic identifier

380703 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Oxidative deamination of D-amino acids By similarity. HAMAP MF_01202
Catalytic activity	A D-amino acid + H2O + acceptor = a 2-oxo acid + NH3 + reduced acceptor. HAMAP MF_01202
Cofactor	FAD By similarity. HAMAP MF_01202
Pathway	Amino-acid degradation; D-alanine degradation; NH(3) and pyruvate from D-alanine: step 1/1. HAMAP MF_01202
Subunit structure	Heterodimer of a small and a large subunit By similarity. HAMAP MF_01202
Sequence similarities	Belongs to the dadA oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanine catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	D-amino-acid dehydrogenase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	D-amino acid dehydrogenase small subunit HAMAP MF_01202		PRO_1000066067
Regions
	Nucleotide binding	3 – 17	15	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	A0KJP1-1 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: CD743CA0707843B2	FASTA	417	44,922
	10 20 30 40 50 60 MDIVVLGGGV VGVTSAWYLA KAGHKVTLLE RRDGVALETS HANAGQISPG YAAPWAAPGI 70 80 90 100 110 120 PLKAAKWLLQ KHAPFTVRPT SDPFQLRWML KMFANCTPAA YAVNKGRMVR LAEYSRDCMK 130 140 150 160 170 180 LLRDELSIDY EGRQLGTLQL FRSQAQLDAS KRDIEVLEEY GVPYQSLDAA GCEGAEPALA 190 200 210 220 230 240 RVRGKIVGGL RLPGDETGDC FRFTKAMAAE AQRLGVKFVF NCAIDEIELA QGRAVAVRAG 250 260 270 280 290 300 EQRFKADAIV CALGSYATGF LRPLGLDLPV YPVKGYSLTL PMINAEASPR STVLDETYKV 310 320 330 340 350 360 AITRFDERIR VGGMAELSGY NLALNPKRHD TLAMVVGDLF PEGGDISRAD FWTGLRPMTP 370 380 390 400 410 DGTPLVGPSP IPGLWLNTGH GTLGWTMAAG SGQLLCDLIS GSDTAISDEG LTLARYG

References

[1]

"Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades." Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F. J. Bacteriol. 188:8272-8282(2006) [PubMed: 16980456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000462 Genomic DNA. Translation: ABK37305.1.
RefSeq	YP_856492.1.
3D structure databases
SMR	A0KJP1. Positions 1-416.
ModBase	Search...
Protein-protein interaction databases
STRING	A0KJP1.
Genome annotation databases
GeneID	4490631.
GenomeReviews	Gene locus AHA_1961 in contig CP000462_GR.
KEGG	aha:AHA_1961.
TIGR	AHA_1961.
Phylogenomic databases
eggNOG	COG0665.
HOGENOM	HBG729204.
OMA	MFQKHAP.
PhylomeDB	A0KJP1.
Family and domain databases
HAMAP	MF_01202. DadA. [Tree]
InterPro	IPR006076. FAD-dep_OxRdtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01266. DAO. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DADA_AERHH

Accession

Primary (citable) accession number: A0KJP1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	December 12, 2006
Last modified:	January 19, 2010
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)