A0KJM0 (PHNX_AERHH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphonoacetaldehyde hydrolase
Short name=Phosphonatase
EC=3.11.1.1

Alternative name(s):
Phosphonoacetaldehyde phosphonohydrolase

Gene names

Name:	phnX
Ordered Locus Names:	AHA_1940

Organism

Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) [Complete proteome] [HAMAP]

Taxonomic identifier

380703 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas › Aeromonas hydrophila › Aeromonas hydrophila subsp. hydrophila

Protein attributes

Sequence length	279 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in phosphonate degradation By similarity.
Catalytic activity	Phosphonoacetaldehyde + H2O = acetaldehyde + phosphate. HAMAP-Rule MF_01375
Cofactor	Binds 1 Mg2+ ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the HAD-like hydrolase superfamily. PhnX family.

Ontologies

Keywords
Ligand	Magnesium Metal-binding Schiff base
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	organic phosphonate catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP phosphonoacetaldehyde hydrolase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 279	279	Phosphonoacetaldehyde hydrolase HAMAP-Rule MF_01375		PRO_0000284573
Sites
	Active site	20	1	Nucleophile By similarity
	Active site	62	1	Schiff-base intermediate with substrate By similarity
	Metal binding	20	1	Magnesium By similarity
	Metal binding	22	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	196	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0KJM0 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 5599F76509E3D2AF	FASTA	279	29,882
	10 20 30 40 50 60 MTDLQIAVDP TTDVEALILD WAGTVVDFGS FAPTSIFVEA FARAYDFPVT LDEARQPMGL 70 80 90 100 110 120 GKWDHIAALG RLPSVDARWQ ARFGHPMSHA EVDHLYHTFM PLQIAAVTRF ADPIPGVLPV 130 140 150 160 170 180 LDALRGQGMK IGSCSGYPRP VMETLVPAAA DHGYRPDHWV ATDDLKAGGR PGPWMALANV 190 200 210 220 230 240 IELGVNAVHR CIKVDDAVPG ISEGLNAGMW TVGLSVSGNE FGATWEAFAA MSEAEIAARR 250 260 270 APAEAKLRAA GAHYVIDTLA DIAPVIADIN RRLAAGERP

References

[1]

"Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades." Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F. J. Bacteriol. 188:8272-8282(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 7966 / NCIB 9240.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000462 Genomic DNA. Translation: ABK38391.1.
RefSeq	YP_856471.1. NC_008570.1.
3D structure databases
ProteinModelPortal	A0KJM0.
ModBase	Search...
Protein-protein interaction databases
STRING	380703.AHA_1940.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABK38391; ABK38391; AHA_1940.
GeneID	4489099.
KEGG	aha:AHA_1940.
PATRIC	20781468. VBIAerHyd135212_1955.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0637.
HOGENOM	HOG000217971.
KO	K05306.
OMA	EFGATWE.
ProtClustDB	PRK13478.
Enzyme and pathway databases
BioCyc	AHYD380703:GH2M-2050-MONOMER.
Family and domain databases
Gene3D	1.10.150.240. 1 hit. 3.40.50.1000. 2 hits.
HAMAP	MF_01375. PhnX.
InterPro	IPR023214. HAD-like_dom. IPR006402. HAD-SF_hydro_IA_v3. IPR023198. PGP_dom2. IPR006323. Phosphonoacetald_hydro. [Graphical view]
Pfam	PF13419. HAD_2. 1 hit. [Graphical view]
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01509. HAD-SF-IA-v3. 1 hit. TIGR01422. phosphonatase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PHNX_AERHH

Accession

Primary (citable) accession number: A0KJM0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 17, 2007
Last sequence update:	December 12, 2006
Last modified:	May 1, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program