Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

GTP cyclohydrolase-2

Gene

ribA

Organism
Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.UniRule annotation

Catalytic activityi

GTP + 3 H2O = formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Pathwayi: riboflavin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-6-(D-ribitylamino)uracil from GTP.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. GTP cyclohydrolase-2 (ribA)
  2. Riboflavin biosynthesis protein RibD (ribD)
  3. Riboflavin biosynthesis protein RibD (ribD)
  4. no protein annotated in this organism
This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-6-(D-ribitylamino)uracil from GTP, the pathway riboflavin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi55 – 551Zinc; catalyticUniRule annotation
Metal bindingi66 – 661Zinc; catalyticUniRule annotation
Metal bindingi68 – 681Zinc; catalyticUniRule annotation
Binding sitei71 – 711GTPUniRule annotation
Binding sitei115 – 1151GTPUniRule annotation
Active sitei127 – 1271Proton acceptorUniRule annotation
Active sitei129 – 1291NucleophileUniRule annotation
Binding sitei150 – 1501GTPUniRule annotation
Binding sitei155 – 1551GTPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi50 – 545GTPUniRule annotation
Nucleotide bindingi93 – 953GTPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Riboflavin biosynthesis

Keywords - Ligandi

GTP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciAHYD380703:GH2M-1807-MONOMER.
UniPathwayiUPA00275; UER00400.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP cyclohydrolase-2UniRule annotation (EC:3.5.4.25UniRule annotation)
Alternative name(s):
GTP cyclohydrolase IIUniRule annotation
Gene namesi
Name:ribAUniRule annotation
Ordered Locus Names:AHA_1807
OrganismiAeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240)
Taxonomic identifieri380703 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeAeromonas
Proteomesi
  • UP000000756 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 197197GTP cyclohydrolase-2PRO_1000203810Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi380703.AHA_1807.

Structurei

3D structure databases

ProteinModelPortaliA0KJ91.
SMRiA0KJ91. Positions 7-172.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GTP cyclohydrolase II family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108JEN. Bacteria.
COG0807. LUCA.
HOGENOMiHOG000115442.
KOiK01497.
OMAiPTPFGVF.

Family and domain databases

CDDicd00641. GTP_cyclohydro2. 1 hit.
HAMAPiMF_00179. RibA. 1 hit.
InterProiIPR032677. GTP_cyclohydro_II.
IPR000926. RibA.
[Graphical view]
PfamiPF00925. GTP_cyclohydro2. 1 hit.
[Graphical view]
SUPFAMiSSF142695. SSF142695. 1 hit.
TIGRFAMsiTIGR00505. ribA. 1 hit.

Sequencei

Sequence statusi: Complete.

A0KJ91-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSVTLVAKS KLPTPWGTFT LVGFQETGTG KDHAALVMGD ITGDEPVLGR
60 70 80 90 100
IHSECLTGDA LFSLRCDCGF QLQAALENIA KAGRGVLLYV RQEGRGIGLL
110 120 130 140 150
NKIRAYHLQD QGADTVEANV ALGFAADMRD YTICADMLKQ LEVKSLKLMT
160 170 180 190
NNPRKMKAME SFGIPVAERV PLQEGRNPHN EFYLSTKANK LDHMFKK
Length:197
Mass (Da):21,643
Last modified:December 12, 2006 - v1
Checksum:i744646D5E3551C5D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000462 Genomic DNA. Translation: ABK36951.1.
RefSeqiWP_010634253.1. NC_008570.1.
YP_856342.1. NC_008570.1.

Genome annotation databases

EnsemblBacteriaiABK36951; ABK36951; AHA_1807.
GeneIDi4487840.
KEGGiaha:AHA_1807.
PATRICi20781196. VBIAerHyd135212_1823.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000462 Genomic DNA. Translation: ABK36951.1.
RefSeqiWP_010634253.1. NC_008570.1.
YP_856342.1. NC_008570.1.

3D structure databases

ProteinModelPortaliA0KJ91.
SMRiA0KJ91. Positions 7-172.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi380703.AHA_1807.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK36951; ABK36951; AHA_1807.
GeneIDi4487840.
KEGGiaha:AHA_1807.
PATRICi20781196. VBIAerHyd135212_1823.

Phylogenomic databases

eggNOGiENOG4108JEN. Bacteria.
COG0807. LUCA.
HOGENOMiHOG000115442.
KOiK01497.
OMAiPTPFGVF.

Enzyme and pathway databases

UniPathwayiUPA00275; UER00400.
BioCyciAHYD380703:GH2M-1807-MONOMER.

Family and domain databases

CDDicd00641. GTP_cyclohydro2. 1 hit.
HAMAPiMF_00179. RibA. 1 hit.
InterProiIPR032677. GTP_cyclohydro_II.
IPR000926. RibA.
[Graphical view]
PfamiPF00925. GTP_cyclohydro2. 1 hit.
[Graphical view]
SUPFAMiSSF142695. SSF142695. 1 hit.
TIGRFAMsiTIGR00505. ribA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRIBA_AERHH
AccessioniPrimary (citable) accession number: A0KJ91
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: December 12, 2006
Last modified: September 7, 2016
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.