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A0KJ55 (NUON_AERHH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADH-quinone oxidoreductase subunit N

EC=1.6.99.5
Alternative name(s):
NADH dehydrogenase I subunit N
NDH-1 subunit N
Gene names
Name:nuoN
Ordered Locus Names:AHA_1770
OrganismAeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) [Complete proteome] [HAMAP]
Taxonomic identifier380703 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeAeromonas

Protein attributes

Sequence length487 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity. HAMAP-Rule MF_00445

Catalytic activity

NADH + quinone = NAD+ + quinol. HAMAP-Rule MF_00445

Subunit structure

NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity.

Subcellular location

Cell inner membrane; Multi-pass membrane protein By similarity HAMAP-Rule MF_00445.

Sequence similarities

Belongs to the complex I subunit 2 family.

Ontologies

Keywords
   Biological processTransport
   Cellular componentCell inner membrane
Cell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   LigandNAD
Ubiquinone
   Molecular functionOxidoreductase
   PTMQuinone
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processATP synthesis coupled electron transport

Inferred from electronic annotation. Source: InterPro

transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionNADH dehydrogenase (ubiquinone) activity

Inferred from electronic annotation. Source: InterPro

quinone binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 487487NADH-quinone oxidoreductase subunit N HAMAP-Rule MF_00445
PRO_0000391091

Regions

Transmembrane8 – 2821Helical; Potential
Transmembrane37 – 5721Helical; Potential
Transmembrane71 – 9121Helical; Potential
Transmembrane104 – 12421Helical; Potential
Transmembrane125 – 14521Helical; Potential
Transmembrane159 – 17921Helical; Potential
Transmembrane203 – 22321Helical; Potential
Transmembrane235 – 25521Helical; Potential
Transmembrane269 – 28921Helical; Potential
Transmembrane303 – 32321Helical; Potential
Transmembrane327 – 34721Helical; Potential
Transmembrane374 – 39421Helical; Potential
Transmembrane408 – 42720Helical; Potential
Transmembrane449 – 46921Helical; Potential

Sequences

Sequence LengthMass (Da)Tools
A0KJ55 [UniParc].

Last modified December 12, 2006. Version 1.
Checksum: 55445AC63FC249F0

FASTA48752,486
        10         20         30         40         50         60 
MTFTASQLLA LLPLLLTTGA MVALMLAIAW KRCDDTAFVV TIAGLNLALF SLPIVMAQGD 

        70         80         90        100        110        120 
QGVTPLLQID GYAVFYMGLV LIGALATCTF GRSWLKGYPD NREEFYLLLL IATAGGLVLA 

       130        140        150        160        170        180 
GSRHLASLFI GIEMLTLPMF GLVGYAYRER HSLEASIKYM VLSAAATAFL LFGMALLYAQ 

       190        200        210        220        230        240 
AGSLSFSDLG LTLATTPAHH PLLMGGLGLM LVGFAFKLSL APFHLWTPDV YEGAPAPVAT 

       250        260        270        280        290        300 
FLATVSKIAV FCVLLRFYLA VPAAADPMIH WLLAAMAVIS IVIGNLLALL QTNIKRMMGY 

       310        320        330        340        350        360 
SSISHFGYLL AVIVASRLGQ MPVEAAGVYL LMYLFTSLGA FGVISMMSSP YRGKDADSLH 

       370        380        390        400        410        420 
SYRGLFWRRP YLTAVLTVMM LSLAGIPMTL GFIGKFYLIG VTVDAKLWWL SGAIVLGSAL 

       430        440        450        460        470        480 
GLYYYLRVMV TLYLREPGMQ LRDAKGDWAI TSGGLVVLLS AALVVALGLY PQPVISLVQG 


FQNVVLH 

« Hide

References

[1]"Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades."
Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F.
J. Bacteriol. 188:8272-8282(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 7966 / NCIB 9240.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000462 Genomic DNA. Translation: ABK39107.1.
RefSeqYP_856306.1. NC_008570.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING380703.AHA_1770.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABK39107; ABK39107; AHA_1770.
GeneID4486788.
KEGGaha:AHA_1770.
PATRIC20781120. VBIAerHyd135212_1786.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1007.
HOGENOMHOG000100796.
KOK00343.
OMASIVQLAM.
OrthoDBEOG64JFNZ.

Enzyme and pathway databases

BioCycAHYD380703:GH2M-1770-MONOMER.

Family and domain databases

HAMAPMF_00445. NDH1_NuoN_1.
InterProIPR010096. NADH-Q_OxRdtase_suN/2.
IPR001750. NADH_UbQ/plastoQ_OxRdtase.
[Graphical view]
PfamPF00361. Oxidored_q1. 1 hit.
[Graphical view]
TIGRFAMsTIGR01770. NDH_I_N. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNUON_AERHH
AccessionPrimary (citable) accession number: A0KJ55
Entry history
Integrated into UniProtKB/Swiss-Prot: February 9, 2010
Last sequence update: December 12, 2006
Last modified: May 14, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families